Parenteral immunization of mice with a genetically inactivated pertussis toxin DNA vaccine induces cell-mediated immunity and protection

Article

Fry, Scott R., Chen, Austen Y., Daggard, Grant and Mukkur, Trilochan K. S.. 2008. "Parenteral immunization of mice with a genetically inactivated pertussis toxin DNA vaccine induces cell-mediated immunity and protection." Journal of Medical Microbiology. 57 (1), pp. 28-35. https://doi.org/10.1099/jmm.0.47527-0
Article Title

Parenteral immunization of mice with a genetically inactivated pertussis toxin DNA vaccine induces cell-mediated immunity and protection

ERA Journal ID15126
Article CategoryArticle
AuthorsFry, Scott R. (Author), Chen, Austen Y. (Author), Daggard, Grant (Author) and Mukkur, Trilochan K. S. (Author)
Journal TitleJournal of Medical Microbiology
Journal Citation57 (1), pp. 28-35
Number of Pages8
Year2008
Place of PublicationUnited Kingdom
ISSN0022-2615
1473-5644
Digital Object Identifier (DOI)https://doi.org/10.1099/jmm.0.47527-0
Web Address (URL)https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.47527-0
Abstract

The immunogenicity and protective efficacy of a DNA vaccine encoding a genetically inactivated S1 domain of pertussis toxin was evaluated using a murine respiratory challenge model of Bordetella pertussis infection. It was found that mice immunized via the intramuscular route elicited a purely cell-mediated immune response to the DNA vaccine, with high levels of gamma interferon (IFN-c) and interleukin (IL)-2 detected in the S1-stimulated splenocyte supernatants and no serum IgG. Despite the lack of an antibody response, the lungs of DNA-immunized mice were cleared of B. pertussis at a significantly faster rate compared with mock-immunized mice following an aerosol challenge. To gauge the true potential of this S1 DNA vaccine, the immune response and protective efficacy of the commercial diphtheria–tetanus–acellular pertussis (DTaP) vaccine were included as the gold standard. Immunization with DTaP elicited a typically strong T-helper (Th)2-polarized immune response with significantly higher titres of serum IgG than in the DNA vaccine group, but a relatively weak Th1 response with low levels of IFN-c and IL-2 detected in the supernatants of antigen-stimulated splenocytes. DTaP-immunized mice cleared the aerosol challenge more efficiently than DNA-immunized mice, with no detectable pathogen after day 7 post-challenge.

KeywordsDNA vaccine; immunization; diptheria pertussis tetanus vaccine; cllular immunity; whooping cough
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 2020320402. Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies)
320701. Medical bacteriology
320603. Medical molecular engineering of nucleic acids and proteins
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Byline AffiliationsDepartment of Biological and Physical Sciences
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