A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci

Article


Zhang, G. W., Kotiw, M. and Daggard, G.. 2002. "A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci." Letters in Applied Microbiology. 35 (3), pp. 247-250. https://doi.org/10.1046/j.1472-765X.2002.01177.x
Article Title

A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci

ERA Journal ID2543
Article CategoryArticle
AuthorsZhang, G. W. (Author), Kotiw, M. (Author) and Daggard, G. (Author)
Journal TitleLetters in Applied Microbiology
Journal Citation35 (3), pp. 247-250
Number of Pages4
Year2002
Place of PublicationUnited Kingdom
ISSN0266-8254
1472-765X
Digital Object Identifier (DOI)https://doi.org/10.1046/j.1472-765X.2002.01177.x
Web Address (URL)https://academic.oup.com/lambio/article/35/3/247/6704620
Abstract

Aims: The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes.

Methods and Results: 30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AGAGGGCACA 3'). Both primers generated DNA electropherotype patterns which, on analysis, clustered the isolates within their respective serotypes. A blind test of a further 3 field isolates also defined these strains within their subsequently determined serotypes. The detection of DNA polymorphisms between isolates within a serotype confirmed previous reports of the heterogenous nature of individual GBS serotypes.

Conclusions: The RAPD-PCR is a potentially useful assay for the rapid characterization of neonatal infections associated with group B streptococci. The method appears to be more discriminatory than conventional serological assays.

Significance and Impact of the Study: The RAPD-PCR assay is faster, more convenient and easier to perform than alternative DNA analytical procedures such as Pulsfield Gel Electrophoresis. We were able to reproduce the same results following re-testing of all isolates some 12 months later which suggests that the assay may be robust enough for use in routine epidemiological investigations.

Keywordsgroup B streptococci; bacterial typing techniques; DNA; genotype; random amplified polymorphic DNA technique; sequence analysis; serotyping; streptococcal infections; streptococcus agalactiae
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 2020320406. Immunogenetics (incl. genetic immunology)
310701. Bacteriology
321399. Paediatrics not elsewhere classified
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Byline AffiliationsDepartment of Biological and Physical Sciences
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Mukkur, T. K. S., Cornford-Nairn, Renee A., Rossetti, T., Kershaw, S., Fry, Scott Robert, Xu, Youhong and Daggard, Grant A.. 2005. "A metabolite-deficient mutant of Bordetella pertussis that induces secretory IgA and cell-mediated immunity in mice vaccinated by the intranasal route." Fang, Ferric C. (ed.) 105th American Society for Microbiology: General Meeting. Atlanta, Georgia 05 - 09 Jun 2005 Washington.
Preparation of total RNA from a very small wheat embryo suitable for differential display
Chen, Yihua, Weining, Song and Daggard, Grant. 2003. "Preparation of total RNA from a very small wheat embryo suitable for differential display ." Annals of Applied Biology. 143 (2), pp. 261-264. https://doi.org/10.1111/j.1744-7348.2003.tb00293.x
Targetting AFLP-DNA markers to specific traits and chromosome regions
Campbell, A. W., Daggard, G., Békés, F., Pedler, A., Sutherland, M. W and Appels, R.. 2001. "Targetting AFLP-DNA markers to specific traits and chromosome regions ." Australian Journal of Agricultural Research. 52 (11-12), pp. 1153-1160. https://doi.org/10.1071/ar01028
Enhanced shoot regeneration in nine Australian wheat cultivars by spermidine and water stress treatments
Khanna, Harjeet K. and Daggard, Grant E.. 2001. "Enhanced shoot regeneration in nine Australian wheat cultivars by spermidine and water stress treatments ." Australian Journal of Plant Physiology. 28 (12), pp. 1243-1247. https://doi.org/10.1071/pp01115
Isochromophilone IX, a novel GABA-containing metabolite isolated from a cultured fungus, Penicillium sp.
Michael, Adam P., Grace, Emily J., Kotiw, Michael and Barrow, Russell A.. 2003. "Isochromophilone IX, a novel GABA-containing metabolite isolated from a cultured fungus, Penicillium sp." Australian Journal of Chemistry: an international journal for chemical science. 56 (1), pp. 13-15. https://doi.org/10.1071/CH02021