A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci
Article
Article Title | A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci |
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ERA Journal ID | 2543 |
Article Category | Article |
Authors | Zhang, G. W. (Author), Kotiw, M. (Author) and Daggard, G. (Author) |
Journal Title | Letters in Applied Microbiology |
Journal Citation | 35 (3), pp. 247-250 |
Number of Pages | 4 |
Year | 2002 |
Place of Publication | United Kingdom |
ISSN | 0266-8254 |
1472-765X | |
Digital Object Identifier (DOI) | https://doi.org/10.1046/j.1472-765X.2002.01177.x |
Web Address (URL) | https://academic.oup.com/lambio/article/35/3/247/6704620 |
Abstract | Aims: The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. Methods and Results: 30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AGAGGGCACA 3'). Both primers generated DNA electropherotype patterns which, on analysis, clustered the isolates within their respective serotypes. A blind test of a further 3 field isolates also defined these strains within their subsequently determined serotypes. The detection of DNA polymorphisms between isolates within a serotype confirmed previous reports of the heterogenous nature of individual GBS serotypes. Conclusions: The RAPD-PCR is a potentially useful assay for the rapid characterization of neonatal infections associated with group B streptococci. The method appears to be more discriminatory than conventional serological assays. Significance and Impact of the Study: The RAPD-PCR assay is faster, more convenient and easier to perform than alternative DNA analytical procedures such as Pulsfield Gel Electrophoresis. We were able to reproduce the same results following re-testing of all isolates some 12 months later which suggests that the assay may be robust enough for use in routine epidemiological investigations. |
Keywords | group B streptococci; bacterial typing techniques; DNA; genotype; random amplified polymorphic DNA technique; sequence analysis; serotyping; streptococcal infections; streptococcus agalactiae |
Contains Sensitive Content | Does not contain sensitive content |
ANZSRC Field of Research 2020 | 320406. Immunogenetics (incl. genetic immunology) |
310701. Bacteriology | |
321399. Paediatrics not elsewhere classified | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Department of Biological and Physical Sciences |
https://research.usq.edu.au/item/9y7yq/a-rapd-pcr-genotyping-assay-which-correlates-with-serotypes-of-group-b-streptococci
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