Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus
Article
Article Title | Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus |
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Article Category | Article |
Authors | Dearnaley, John (Author) and Daggard, Grant (Author) |
Journal Title | Sexual Plant Reproduction |
Journal Citation | 13 (5), pp. 265-271 |
Year | 2001 |
Place of Publication | Germany |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s004970000062 |
Web Address (URL) | https://link.springer.com/article/10.1007/s004970000062 |
Abstract | Penetration of pollen tubes through stigmatic tissues in Brassica napus L. may involve the release of cell wall modifying enzymes from the pollen tube tip. We have examined the expression of a pectin-degrading polygalacturonase (PG) enzyme in unpollinated and early and late pollinated stigmas via immunoblotting and immuno light microscopy using a PG polyclonal antibody. Immunoblotting analysis indicated that PG enzyme was present at low levels in unpollinated stigmas and at high levels in pollinated stigmas. The level of PG did not detectably increase between early and late pollinated stigmas. Immuno light microscopy demonstrated that PG enzyme was present in ungerminated pollen grains, stigmatic papillae and in the tip of pollen tubes growing into the papillar wall. This latter evidence suggests that PG enzyme may play an important role in papillar cell wall penetration during pollination although other interpretations of the role of pollen PG should not be discounted. |
Keywords | Brassica napus, pollen tubes, stigma, pollination, polygalacturonase |
Contains Sensitive Content | Does not contain sensitive content |
ANZSRC Field of Research 2020 | 310803. Plant cell and molecular biology |
310899. Plant biology not elsewhere classified | |
310804. Plant developmental and reproductive biology | |
Public Notes | File reproduced in accordance with the copyright policy of the publisher/author. |
Byline Affiliations | Department of Biological and Physical Sciences |
https://research.usq.edu.au/item/9y4qz/expression-of-a-polygalacturonase-enzyme-in-germinating-pollen-of-brassica-napus
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