An investigation of the ecology and bioactivity of endophytes from Pittosporum angustifolium

Poster

Thompson, M., Lynch, M. and Dearnaley, J.. 2014. "An investigation of the ecology and bioactivity of endophytes from Pittosporum angustifolium." 2014 Scientific Meeting of the Australasian Mycological Society. Brisbane, Australia 21 - 23 Apr 2014 Australia.
Paper/Presentation Title

An investigation of the ecology and bioactivity of endophytes from Pittosporum angustifolium

Presentation TypePoster
AuthorsThompson, M. (Author), Lynch, M. (Author) and Dearnaley, J. (Author)
Number of Pages1
Year2014
Place of PublicationAustralia
Web Address (URL) of Paperhttp://www.australasianmycologicalsociety.com/past-meetings-2014
Conference/Event2014 Scientific Meeting of the Australasian Mycological Society
Event Details
2014 Scientific Meeting of the Australasian Mycological Society
Event Date
21 to end of 23 Apr 2014
Event Location
Brisbane, Australia
Abstract

Knowledge of the ecology and antimicrobial activity of endophytes within Australian plants is currently limited. This project investigated the endophytes of Pittosporum angustifolium; an Australian plant known for its medicinal use by indigenous Australians and from which antimicrobial chemicals have been extracted (from the essential oils of its leaves and fruit). Plants were sampled from seven sites in SE Queensland. Endophytes were cultured from plant tissue onto potato dextrose agar. Sequencing of ITS-DNA regions of fungal endophytes and SSU-DNA regions of bacterial endophytes revealed a variety of different species isolated from plants sampled at the different sites - evidence against any host specific interactions. Primary screening of isolates occurred on Sensitest agar against ATCC type strains of Staphylococcus aureus, Serratia marcescens, Escherichia coli and Candida albicans. Primary screening resulted in strains of Pseudocercospora fuligena being identified as candidates for further investigation. Work is still ongoing to confirm the bioactivity of the P. fuligena isolate and to identify any bioactive compounds produced.

Keywordsendophyte, Pittosporum angustifolium, bioactivity
ANZSRC Field of Research 2020310705. Mycology
Public Notes

Poster presentation - only Abstract printed, as provided here.

Byline AffiliationsSchool of Agricultural, Computational and Environmental Sciences
Institution of OriginUniversity of Southern Queensland
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Phosphate transporter expression in the soybean AM symbiosis
Dearnaley, John, Learmonth, Barbara A. and Bougoure, Damian S.. 2002. "Phosphate transporter expression in the soybean AM symbiosis." 7th International Mycological Congress (IMC7). Oslo, Norway 11 - 17 Aug 2002 Australia.
Orchid mycorrhizas - a disproportionate focus on the minutiae?
Dearnaley, John. 2010. "Orchid mycorrhizas - a disproportionate focus on the minutiae?" 2010 Joint Australasian Mycological Society and the Australian Society for Mycrobiology Meeting. Sydney, Australia 04 - 08 Jul 2010
Molecular identification of mycorrhizal fungi in Australian threatened plant species
Dearnaley, John D. W., Downie, Alex F., Murray, Andrew J. and Le Brocque, Andrew F.. 2008. "Molecular identification of mycorrhizal fungi in Australian threatened plant species." Schenk, Peer (ed.) 1st International Conference on Biotic Plant Interactions (ICBPI 2008). Brisbane, Australia 27 - 29 Mar 2008 Brisbane, Australia.
Molecular identification of a mycorrhizal Sebacinaceae from the endangered Caladenia atroclavia (black clubbed spider orchid)
Dearnaley, J. D. W., Murray, A. J. and Mathieson, M. T.. 2009. "Molecular identification of a mycorrhizal Sebacinaceae from the endangered Caladenia atroclavia (black clubbed spider orchid)." Australasian Mycologist. 28 (2-3), pp. 45-50.
Defence gene expression in single mycorrhizal roots of soybean
Dearnaley, John and Learmonth, Barbara A.. 2003. "Defence gene expression in single mycorrhizal roots of soybean." 4th International Conference on Mycorrhizae (ICOM4) . Montreal, Canada 10 - 15 Aug 2003 Québec, Canada.
The mycorrhizal fungal associations of the vunerable Sophora fraseri (Fabaceae)
Downie, A. F., Le Brocque, A. F. and Dearnaley, J. D. W.. 2008. "The mycorrhizal fungal associations of the vunerable Sophora fraseri (Fabaceae)." Australasian Mycologist. 27 (2), pp. 57-65.
Lantana invades the soil too! Effects of Lantana camara on soil fungal dominance
Case, Heidi, Dearnaley, John and Le Brocque, Andrew F.. 2008. "Lantana invades the soil too! Effects of Lantana camara on soil fungal dominance." 2008 Conference of the Ecological Society of Australia (ESA 2008). Sydney, Australia 01 - 05 Dec 2008 Australia. Ecological Society of Australia.
Endophytic fungi associated with Australian orchids
Dearnaley, John and Le Brocque, Andrew F.. 2006. "Endophytic fungi associated with Australian orchids." Australasian Plant Conservation: Bulletin of the Australian Network for Plant Conservation. 15 (2), pp. 7-9.
Molecular identification of fungal endophytes in Australian myco-heterotrophic orchids
Dearnaley, John. 2006. "Molecular identification of fungal endophytes in Australian myco-heterotrophic orchids." Meyer, Wieland (ed.) 8th International Mycological Congress (IMC8). Cairns, Australia 20 - 25 Aug 2006 Australia.
Fungal endophytes in Australian myco-heterotrophic orchids
Dearnaley, John. 2006. "Fungal endophytes in Australian myco-heterotrophic orchids." Meyer, Wieland (ed.) 8th International Mycological Congress (IMC8). Cairns, Australia 20 - 25 Aug 2006 Australia.
The fungal endophytes of erythrorchis cassythoides - is this orchid saprophytic or parasitic?
Dearnaley, John. 2006. "The fungal endophytes of erythrorchis cassythoides - is this orchid saprophytic or parasitic?" Australasian Mycologist. 25 (2), pp. 51-57.
The fungal endophytes of dipodium variegatum (orchidaceae)
Bougoure, Jeremy J. and Dearnaley, John. 2005. "The fungal endophytes of dipodium variegatum (orchidaceae)." Australasian Mycologist. 24 (1), pp. 15-19.
ITS-RFLP and sequence analysis of endophytes from Acianthus, Caladenia and Pterostylis (orchidaceae) in southeastern Queensland
Bougoure, Jeremy J., Bougoure, Damian S., Cairney, John W. G. and Dearnaley, John D. W.. 2005. "ITS-RFLP and sequence analysis of endophytes from Acianthus, Caladenia and Pterostylis (orchidaceae) in southeastern Queensland." Mycological Research. 109 (4), pp. 452-460. https://doi.org/10.1017/S095375620500225X