Phosphate transporter expression in the soybean AM symbiosis

Paper

Dearnaley, John, Learmonth, Barbara A. and Bougoure, Damian S.. 2002. "Phosphate transporter expression in the soybean AM symbiosis." 7th International Mycological Congress (IMC7). Oslo, Norway 11 - 17 Aug 2002 Australia.
Paper/Presentation Title

Phosphate transporter expression in the soybean AM symbiosis

Presentation TypePaper
AuthorsDearnaley, John (Author), Learmonth, Barbara A. (Author) and Bougoure, Damian S. (Author)
Journal or Proceedings TitleBook of Abstracts from the 7th International Mycological Congress
Year2002
Place of PublicationAustralia
Web Address (URL) of Paperhttp://www.ima-mycology.org/doc/IMC7_Book_of_Abstracts.pdf
Conference/Event7th International Mycological Congress (IMC7)
Event Details
7th International Mycological Congress (IMC7)
Event Date
11 to end of 17 Aug 2002
Event Location
Oslo, Norway
Abstract

We are currently studying genes that are potentially involved in the process of phosphate uptake in the arbuscular mycorrhizal (AM) symbiosis of soybean. We have extracted mRNA from AM colonised and uncolonised soybean roots using biotinylated poly-T probes and streptavidin-coated magnetic particles. Total mRNA extracted by this method was reverse-transcribed into cDNA with poly-T primer and reverse transcriptase. cDNA was next PCR-amplified with primers for a previously published soybean root phosphate transporter gene and amplified sequences were visualized on silver-stained acrylamide gels. In situ hybridization studies were carried out to localise the phosphate transporter in root tissues. Root cryosections were probed with a fluorosceinconjugated antisense probe and bound targets were visualized either via alkaline phosphatase labeling or with an antifluoroscein texas-red antibody. Results of these investigations are presented here.

Keywordssoybean; phosphate uptake; mycorrhizal symbiosis
ANZSRC Field of Research 2020310599. Genetics not elsewhere classified
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Byline AffiliationsDepartment of Biological and Physical Sciences
Office of Research
University of Western Sydney
Institution of OriginUniversity of Southern Queensland
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Dearnaley, J. D. W., Murray, A. J. and Mathieson, M. T.. 2009. "Molecular identification of a mycorrhizal Sebacinaceae from the endangered Caladenia atroclavia (black clubbed spider orchid)." Australasian Mycologist. 28 (2-3), pp. 45-50.
Defence gene expression in single mycorrhizal roots of soybean
Dearnaley, John and Learmonth, Barbara A.. 2003. "Defence gene expression in single mycorrhizal roots of soybean." 4th International Conference on Mycorrhizae (ICOM4) . Montreal, Canada 10 - 15 Aug 2003 Québec, Canada.
The mycorrhizal fungal associations of the vunerable Sophora fraseri (Fabaceae)
Downie, A. F., Le Brocque, A. F. and Dearnaley, J. D. W.. 2008. "The mycorrhizal fungal associations of the vunerable Sophora fraseri (Fabaceae)." Australasian Mycologist. 27 (2), pp. 57-65.
Lantana invades the soil too! Effects of Lantana camara on soil fungal dominance
Case, Heidi, Dearnaley, John and Le Brocque, Andrew F.. 2008. "Lantana invades the soil too! Effects of Lantana camara on soil fungal dominance." 2008 Conference of the Ecological Society of Australia (ESA 2008). Sydney, Australia 01 - 05 Dec 2008 Australia. Ecological Society of Australia.
The tetrazolium dyes MTS and XTT provide new quantitative assays for superoxide and superoxide dismutase
Sutherland, Mark W. and Learmonth, Barbara A.. 1997. "The tetrazolium dyes MTS and XTT provide new quantitative assays for superoxide and superoxide dismutase." Free Radical Research. 27 (3), pp. 283-289. https://doi.org/10.3109/10715769709065766
Endophytic fungi associated with Australian orchids
Dearnaley, John and Le Brocque, Andrew F.. 2006. "Endophytic fungi associated with Australian orchids." Australasian Plant Conservation: Bulletin of the Australian Network for Plant Conservation. 15 (2), pp. 7-9.
Molecular identification of fungal endophytes in Australian myco-heterotrophic orchids
Dearnaley, John. 2006. "Molecular identification of fungal endophytes in Australian myco-heterotrophic orchids." Meyer, Wieland (ed.) 8th International Mycological Congress (IMC8). Cairns, Australia 20 - 25 Aug 2006 Australia.
Fungal endophytes in Australian myco-heterotrophic orchids
Dearnaley, John. 2006. "Fungal endophytes in Australian myco-heterotrophic orchids." Meyer, Wieland (ed.) 8th International Mycological Congress (IMC8). Cairns, Australia 20 - 25 Aug 2006 Australia.
The fungal endophytes of erythrorchis cassythoides - is this orchid saprophytic or parasitic?
Dearnaley, John. 2006. "The fungal endophytes of erythrorchis cassythoides - is this orchid saprophytic or parasitic?" Australasian Mycologist. 25 (2), pp. 51-57.
The fungal endophytes of dipodium variegatum (orchidaceae)
Bougoure, Jeremy J. and Dearnaley, John. 2005. "The fungal endophytes of dipodium variegatum (orchidaceae)." Australasian Mycologist. 24 (1), pp. 15-19.
ITS-RFLP and sequence analysis of endophytes from Acianthus, Caladenia and Pterostylis (orchidaceae) in southeastern Queensland
Bougoure, Jeremy J., Bougoure, Damian S., Cairney, John W. G. and Dearnaley, John D. W.. 2005. "ITS-RFLP and sequence analysis of endophytes from Acianthus, Caladenia and Pterostylis (orchidaceae) in southeastern Queensland." Mycological Research. 109 (4), pp. 452-460. https://doi.org/10.1017/S095375620500225X