Molecular identification of mycorrhizal Russulaceae fungi

Presentation

Boddington, Morwenna, Dearnaley, John D., Lebel, Teresa and Leonard, Patrick. 2015. "Molecular identification of mycorrhizal Russulaceae fungi." 2015 Scientific Meeting of the Australasian Mycological Society. Canberra, Australia 14 - 16 Jul 2015 Australia.
Paper/Presentation Title

Molecular identification of mycorrhizal Russulaceae fungi

Presentation TypePresentation
AuthorsBoddington, Morwenna (Author), Dearnaley, John D. (Author), Lebel, Teresa (Author) and Leonard, Patrick (Author)
Journal or Proceedings Title2015 Scientific Meeting of the Australasian Mycological Society: Program and Abstract Book
Article Number25626
Number of Pages1
Year2015
Place of PublicationAustralia
Web Address (URL) of Paperhttp://www.australasianmycologicalsociety.com/past-meetings-2015
Conference/Event2015 Scientific Meeting of the Australasian Mycological Society
Event Details
2015 Scientific Meeting of the Australasian Mycological Society
Event Date
14 to end of 16 Jul 2015
Event Location
Canberra, Australia
Abstract

The Russulaceae is a family of basidiomycetous fungi found in a variety of habitats around the world. While reasonably common in Australian temperate forests, little work has been conducted on this group, with the result that many taxa are undescribed or poorly documented. The aim of
this research project is to improve this situation.
The identification of Russulaceae fruiting bodies to species level has many challenges and is predominantly based on characteristics that are highly subjective, changeable, require specialised chemicals, and specialised equipment. A certain amount of expertise is also required. However, even this is no guarantee of consistently obtaining a positive species identification.
Molecular analysis potentially offers a non-subjective method of identification. In particular, the internal transcribed spacer (ITS) region of fungal DNA has been proposed as the primary standard molecular benchmark for identification of fungi. Coupled with access to millions of sequences held in databases such as GenBank, it would seem that molecular identification offers a useful opportunity to streamline and improve the identification of the Russulaceae. However, this is not always the case, and in sequencing the ITS region of numerous specimens from this family a number of issues have arisen. This presentation will examine some of the difficulties experienced in the molecular identification of Australian Russulaceae species and discuss some of the potential solutions.

Keywordsmycorrhizal Russulaceae fungi
ANZSRC Field of Research 2020310705. Mycology
Public Notes

Oral presentation - only Abstract printed, as provided here.

Byline AffiliationsFaculty of Health, Engineering and Sciences
School of Agricultural, Computational and Environmental Sciences
Manaaki Whenua – Landcare Research, New Zealand
No affiliation
Institution of OriginUniversity of Southern Queensland
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Dearnaley, John, Learmonth, Barbara A. and Bougoure, Damian S.. 2002. "Phosphate transporter expression in the soybean AM symbiosis." 7th International Mycological Congress (IMC7). Oslo, Norway 11 - 17 Aug 2002 Australia.
Orchid mycorrhizas - a disproportionate focus on the minutiae?
Dearnaley, John. 2010. "Orchid mycorrhizas - a disproportionate focus on the minutiae?" 2010 Joint Australasian Mycological Society and the Australian Society for Mycrobiology Meeting. Sydney, Australia 04 - 08 Jul 2010
Molecular identification of mycorrhizal fungi in Australian threatened plant species
Dearnaley, John D. W., Downie, Alex F., Murray, Andrew J. and Le Brocque, Andrew F.. 2008. "Molecular identification of mycorrhizal fungi in Australian threatened plant species." Schenk, Peer (ed.) 1st International Conference on Biotic Plant Interactions (ICBPI 2008). Brisbane, Australia 27 - 29 Mar 2008 Brisbane, Australia.
Molecular identification of a mycorrhizal Sebacinaceae from the endangered Caladenia atroclavia (black clubbed spider orchid)
Dearnaley, J. D. W., Murray, A. J. and Mathieson, M. T.. 2009. "Molecular identification of a mycorrhizal Sebacinaceae from the endangered Caladenia atroclavia (black clubbed spider orchid)." Australasian Mycologist. 28 (2-3), pp. 45-50.
Defence gene expression in single mycorrhizal roots of soybean
Dearnaley, John and Learmonth, Barbara A.. 2003. "Defence gene expression in single mycorrhizal roots of soybean." 4th International Conference on Mycorrhizae (ICOM4) . Montreal, Canada 10 - 15 Aug 2003 Québec, Canada.
The mycorrhizal fungal associations of the vunerable Sophora fraseri (Fabaceae)
Downie, A. F., Le Brocque, A. F. and Dearnaley, J. D. W.. 2008. "The mycorrhizal fungal associations of the vunerable Sophora fraseri (Fabaceae)." Australasian Mycologist. 27 (2), pp. 57-65.
Lantana invades the soil too! Effects of Lantana camara on soil fungal dominance
Case, Heidi, Dearnaley, John and Le Brocque, Andrew F.. 2008. "Lantana invades the soil too! Effects of Lantana camara on soil fungal dominance." 2008 Conference of the Ecological Society of Australia (ESA 2008). Sydney, Australia 01 - 05 Dec 2008 Australia. Ecological Society of Australia.
Endophytic fungi associated with Australian orchids
Dearnaley, John and Le Brocque, Andrew F.. 2006. "Endophytic fungi associated with Australian orchids." Australasian Plant Conservation: Bulletin of the Australian Network for Plant Conservation. 15 (2), pp. 7-9.
Molecular identification of fungal endophytes in Australian myco-heterotrophic orchids
Dearnaley, John. 2006. "Molecular identification of fungal endophytes in Australian myco-heterotrophic orchids." Meyer, Wieland (ed.) 8th International Mycological Congress (IMC8). Cairns, Australia 20 - 25 Aug 2006 Australia.
Fungal endophytes in Australian myco-heterotrophic orchids
Dearnaley, John. 2006. "Fungal endophytes in Australian myco-heterotrophic orchids." Meyer, Wieland (ed.) 8th International Mycological Congress (IMC8). Cairns, Australia 20 - 25 Aug 2006 Australia.
The fungal endophytes of erythrorchis cassythoides - is this orchid saprophytic or parasitic?
Dearnaley, John. 2006. "The fungal endophytes of erythrorchis cassythoides - is this orchid saprophytic or parasitic?" Australasian Mycologist. 25 (2), pp. 51-57.
The fungal endophytes of dipodium variegatum (orchidaceae)
Bougoure, Jeremy J. and Dearnaley, John. 2005. "The fungal endophytes of dipodium variegatum (orchidaceae)." Australasian Mycologist. 24 (1), pp. 15-19.
ITS-RFLP and sequence analysis of endophytes from Acianthus, Caladenia and Pterostylis (orchidaceae) in southeastern Queensland
Bougoure, Jeremy J., Bougoure, Damian S., Cairney, John W. G. and Dearnaley, John D. W.. 2005. "ITS-RFLP and sequence analysis of endophytes from Acianthus, Caladenia and Pterostylis (orchidaceae) in southeastern Queensland." Mycological Research. 109 (4), pp. 452-460. https://doi.org/10.1017/S095375620500225X