The diversity and antimicrobial activity of Preussia sp. Endophytes isolated from Australian dry rainforests
Article
Article Title | The diversity and antimicrobial activity of Preussia sp. Endophytes isolated from Australian dry rainforests |
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ERA Journal ID | 2464 |
Article Category | Article |
Authors | Mapperson, Rachel R. (Author), Kotiw, Michael (Author), Davis, Rohan A. (Author) and Dearnaley, John D. W. (Author) |
Journal Title | Current Microbiology: an international journal |
Journal Citation | 68 (1), pp. 30-37 |
Number of Pages | 8 |
Year | 2014 |
Place of Publication | New York, NY. United States |
ISSN | 0343-8651 |
1432-0991 | |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s00284-013-0415-5 |
Web Address (URL) | http://link.springer.com/content/pdf/10.1007%2Fs00284-013-0415-5.pdf |
Abstract | Limited knowledge currently exists regarding species diversity and antimicrobial activity of endophytic isolates of Preussia within Australia. This report describes endophytic Preussia species that were identified through molecular analysis of the internal transcribed spacer region. Screening for antimicrobial secondary metabolites was determined by testing crude ethyl acetate (EtOAc) extracts derived from fungal mycelia against a panel of ATCC type strains which included Bacillus cereus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Serratia marcescens, methicillin-resistant Staphylococcus aureus (MRSA) and the opportunist yeast pathogen Candida albicans. Subsequently, high-performance liquid chromatography generated fractions of bioactive EtOAc extracts which were subject to confirmatory testing using the Clinical and Laboratory Standards Institute reference microdilution antimicrobial activity assay. A total of 18 Preussia were isolated from nine host plants with 6/18 having a <97% sequence similarity to other known species in Genbank, suggesting that they are new species. In preliminary screening, 13/18 Preussia isolates revealed antimicrobial activity against at least one of the microbes tested, whilst 6/18 isolates, including 4/6 putative new species showed specific antimicrobial activity against MRSA and C. albicans. These results highlight the antimicrobial potential of Australian Preussia spp. and also the importance of Australian dry rainforests as an untapped repository of potentially significant bioactive compounds. |
Keywords | fungal mycelia; assay; rainforest plants; antimicrobial potential |
ANZSRC Field of Research 2020 | 310805. Plant pathology |
310701. Bacteriology | |
340403. Characterisation of biological macromolecules | |
Public Notes | © 2013 Springer Science+Business Media New York. Permanent restricted access to Published version due to publisher copyright policy. |
Byline Affiliations | Australian Centre for Sustainable Catchments |
School of Health and Wellbeing | |
Griffith University | |
Faculty of Health, Engineering and Sciences | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q20yv/the-diversity-and-antimicrobial-activity-of-preussia-sp-endophytes-isolated-from-australian-dry-rainforests
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