The diversity and antimicrobial activity of Preussia sp. Endophytes isolated from Australian dry rainforests
Article
Article Title | The diversity and antimicrobial activity of Preussia sp. Endophytes isolated from Australian dry rainforests |
---|---|
ERA Journal ID | 2464 |
Article Category | Article |
Authors | Mapperson, Rachel R. (Author), Kotiw, Michael (Author), Davis, Rohan A. (Author) and Dearnaley, John D. W. (Author) |
Journal Title | Current Microbiology: an international journal |
Journal Citation | 68 (1), pp. 30-37 |
Number of Pages | 8 |
Year | 2014 |
Place of Publication | New York, NY. United States |
ISSN | 0343-8651 |
1432-0991 | |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s00284-013-0415-5 |
Web Address (URL) | http://link.springer.com/content/pdf/10.1007%2Fs00284-013-0415-5.pdf |
Abstract | Limited knowledge currently exists regarding species diversity and antimicrobial activity of endophytic isolates of Preussia within Australia. This report describes endophytic Preussia species that were identified through molecular analysis of the internal transcribed spacer region. Screening for antimicrobial secondary metabolites was determined by testing crude ethyl acetate (EtOAc) extracts derived from fungal mycelia against a panel of ATCC type strains which included Bacillus cereus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Serratia marcescens, methicillin-resistant Staphylococcus aureus (MRSA) and the opportunist yeast pathogen Candida albicans. Subsequently, high-performance liquid chromatography generated fractions of bioactive EtOAc extracts which were subject to confirmatory testing using the Clinical and Laboratory Standards Institute reference microdilution antimicrobial activity assay. A total of 18 Preussia were isolated from nine host plants with 6/18 having a <97% sequence similarity to other known species in Genbank, suggesting that they are new species. In preliminary screening, 13/18 Preussia isolates revealed antimicrobial activity against at least one of the microbes tested, whilst 6/18 isolates, including 4/6 putative new species showed specific antimicrobial activity against MRSA and C. albicans. These results highlight the antimicrobial potential of Australian Preussia spp. and also the importance of Australian dry rainforests as an untapped repository of potentially significant bioactive compounds. |
Keywords | fungal mycelia; assay; rainforest plants; antimicrobial potential |
ANZSRC Field of Research 2020 | 310805. Plant pathology |
310701. Bacteriology | |
340403. Characterisation of biological macromolecules | |
Public Notes | © 2013 Springer Science+Business Media New York. Permanent restricted access to Published version due to publisher copyright policy. |
Byline Affiliations | Australian Centre for Sustainable Catchments |
School of Health and Wellbeing | |
Griffith University | |
Faculty of Health, Engineering and Sciences | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q20yv/the-diversity-and-antimicrobial-activity-of-preussia-sp-endophytes-isolated-from-australian-dry-rainforests
1880
total views6
total downloads0
views this month0
downloads this month
Export as
Related outputs
Tree shade within urban parks and nature strips: standards and perspectives from measurement campaigns conducted in Queensland
Downs, Nathan, Antrobus, Jodie, Dekeyser, Stijn, Butler, Harry, Downs, Melanie, Hickmott, Darren, Madigan, Chris, Brown, Alexandra, Amar, Abdurazaq, Dearnaley, John, Parisi, Alfio V, Vanos, Jennifer, Raj, Nawin, Harrison, Simone and Igoe, Damien. 2024. "Tree shade within urban parks and nature strips: standards and perspectives from measurement campaigns conducted in Queensland ." 6th International conference UV and Skin Cancer Prevention. Brisbane, Australia 11 - 15 Sep 2024 Australia.Strike 3 … Out! Investigating Pre-Game Moods, Performance, and Mental Health of Softball Umpires
Houison, Ronald J., Lamont-Mills, Andrea, Kotiw, Michael and Terry, Peter C.. 2024. "Strike 3 … Out! Investigating Pre-Game Moods, Performance, and Mental Health of Softball Umpires." Sports. 12 (2). https://doi.org/10.3390/sports12020050Developing a selective culturing approach for Campylobacter hepaticus
Sakur, Sheaaz G. J., Williamson, Sarah L., Pavic, Anthony, Gao, Yuanshuo K., Harris, Taha, Kotiw, Michael, Muir, Wendy Isabelle and Groves, Peter John. 2024. "Developing a selective culturing approach for Campylobacter hepaticus." PLoS One. 19 (5). https://doi.org/10.1371/journal.pone.0302861Descriptive epidemiology of Spotty Liver Disease in Australian cage-free brown egg layer chicken flocks with a scratch area
Groves, Peter J., Gao, Yuanshuo K., Kotiw, Michael, Eastwood, Sarah, Van, T. T. Hao Van, Moore, Robert J. and Muir, Wendy I.. 2024. "Descriptive epidemiology of Spotty Liver Disease in Australian cage-free brown egg layer chicken flocks with a scratch area." Poultry Science. 103 (8). https://doi.org/10.1016/j.psj.2024.103941Chimeric hepatitis B surface antigen virus-like particles expressing the strep A epitope p*17 elicit a humoral immune response in mice
Dooley, Leanne, Ahmad, Tarek, Ozberk, Victoria, Pandey, Manisha, Good, Michael and Kotiw, Mkie. 2024. "Chimeric hepatitis B surface antigen virus-like particles expressing the strep A epitope p*17 elicit a humoral immune response in mice ." Heliyon. 10 (9). https://doi.org/10.1016/j.heliyon.2024.e30606The mitigating effect of street trees, urban flora, and the suburban environment on seasonal peak UV indices: A case study from Brisbane, Australia
Downs, Nathan James, Amar, Abdurazaq, Dearnaley, John, Butler, Harry, Dekeyser, Stijn, Igoe, Damien, Parisi, Alfio V., Raj, Nawin, Deo, Ravinesh and Turner, Joanna. 2024. "The mitigating effect of street trees, urban flora, and the suburban environment on seasonal peak UV indices: A case study from Brisbane, Australia." Photochemistry and Photobiology. https://doi.org/10.1111/php.13988Feeling the Stress: Salivary Cortisol Responses of Softball Umpires during National Championships
Houison, Ronald J, Lamont-Mills, Andrea, Kotiw, Michael and Terry, Peter C.. 2024. "Feeling the Stress: Salivary Cortisol Responses of Softball Umpires during National Championships." Sports. 12 (5). https://doi.org/10.3390/sports12050128Integrating soil microbial communities into fundamental ecology, conservation, and restoration: examples from Australia
Birnbaum, Christina, Dearnaley, John, Egidi, Eleonora, Frew, Adam, Hopkins, Anna, Powell, Jeff, Aguilar-Trigueros, Carlos, Liddicoat, Craig, Albornoz, Felipe, Heuck, Meike K., Dadzie, Frederick A., Florence, Luke, Singh, Pankaj, Mansfield, Tomas, Rajapaksha, Kumari, Stewart, Jana, Rallo, Paola, Peddle, Shawn D. and Chiarenza, Giancarlo. 2024. "Integrating soil microbial communities into fundamental ecology, conservation, and restoration: examples from Australia." New Phytologist. 241 (3), pp. 974-981. https://doi.org/10.1111/nph.19440From invisible to visible: Terrestrial biodiversity assessments and conservation policies must include soil organisms in Australia
Birnbaum, Christina and Dearnaley, John. 2023. "From invisible to visible: Terrestrial biodiversity assessments and conservation policies must include soil organisms in Australia." Science. 381 (6658). https://doi.org/10.1126/science.adg7870Identification of epidemiological risk factors for spotty liver disease in cage-free layer flocks in houses with fully slatted flooring in Australia
Gao, Yuanshuo K., Singh, Mini, Muir, Wendy I., Kotiw, Michael and Groves, Peter J.. 2023. "Identification of epidemiological risk factors for spotty liver disease in cage-free layer flocks in houses with fully slatted flooring in Australia." Poultry Science. 102 (12). https://doi.org/10.1016/j.psj.2023.103139Corrigendum to: Marasmius australotrichotus (Marasmiaceae), a new setose species from Australia, and an intriguing range extension for M. paratrichotus
Guard, Frances, Dearnaley, John, Lebel, Teresa, Barrett, Matthew D. and Bougher, Neale L.. 2023. "Corrigendum to: Marasmius australotrichotus (Marasmiaceae), a new setose species from Australia, and an intriguing range extension for M. paratrichotus." Nuytsia. 34, pp. 295-297. https://doi.org/10.58828/nuy01066Scratch area as an epidemiological risk factor for Spotty Liver Disease in cage-free layers in Australia
Gao, Yuanshuo K., Singh, Mini, Muir, Wendy I., Kotiw, Michael and Groves, Peter J.. 2023. "Scratch area as an epidemiological risk factor for Spotty Liver Disease in cage-free layers in Australia." Poultry Science. 102 (10). https://doi.org/10.1016/j.psj.2023.102922Marasmius australotrichotus (Marasmiaceae), a new setose species from Australia, and an intriguing range extension for M. paratrichotus
Dearnaley, John, Guard, Frances E., Lebel, Teresa, Barrett, Matt D. and Bougher, Neale L.. 2023. "Marasmius australotrichotus (Marasmiaceae), a new setose species from Australia, and an intriguing range extension for M. paratrichotus." Nuytsia. 34, pp. 203-219. https://doi.org/10.58828/nuy01059Detection, characterization, and persistence of Campylobacter hepaticus, the cause of spotty liver disease in layer hens
Courtice, Jodi M., Ahmad, Tarek B., Wei, Chuanyu, Mahdi, Layla K., Palmieri, Chiara, Juma, Sarah, Groves, Peter J., Hancock, Kerry, Korolik, Victoria, Petrovsky, Nikolai and Kotiw, Michael. 2023. "Detection, characterization, and persistence of Campylobacter hepaticus, the cause of spotty liver disease in layer hens." Poultry Science. 102 (7). https://doi.org/10.1016/j.psj.2022.102462Construction and Deconstruction: Materials and substances from 'waste'
Burey, Paulomi, Feldman, Jessica, Seligmann, Hannah, Song, Eric, Flynn, Matthew, Helwig, Andreas, Gharineiat, Zahra, Seneviratne, Dinuki, Whiteside, Eliza, Shelley, Tristan, Priesler, Nils, Manalo, Allan, Mirzaghorbanali, Ali, Nourizadeh, Hadi, Roberts, Michae, Nicol, Rose, Redmond, Petrea, Lynch, Mark, Dearnaley, John, ..., Germon, Geoff. 2023. "Construction and Deconstruction: Materials and substances from 'waste'." Chemistry in Australia. (June-August 2023), pp. 16-21.Fungal Planet description sheets: 1383–1435
Crous, P. W., Boers, J., Holdom, D., Osieck, E. R., Steinrucken, T. V., Tan, Y. P., Vitelli, J. S., Shivas, R. G., Barrett, M., Broadbridge, J., Larsson, E., Lebel, T., Pinruan, U., Sommai, S., Alvarado, P., Bonito, G., Decock, C. A., De la Pena-Lastra, S., Delgado, G., ..., Groenewald, J. Z.. 2022. "Fungal Planet description sheets: 1383–1435." Persoonia. 48, pp. 1-153. https://doi.org/10.3767/persoonia.2022.48.08Fungal Planet description sheets: 1436–1477
Tan, Y.P., Bishop-Hurley, S.L., Shivas, R.G., Cowan, D.A., Maggs-Kolling, G., Maharachchikumbura, S.S.N., Pinruan, U., Bransgrove, K.L., De la Pena-Lastra, S., Larsson, E., Lebel, T., Mahadevakumar, S., Mateos, A., Osieck, E.R., Rigueiro-Rodriguez, A., Sommai, S., Ajithkumar, K., Akulov, A., Anderson, F.E., ..., Crous, P.W.. 2022. "Fungal Planet description sheets: 1436–1477." Persoonia. 49, pp. 261-350. https://doi.org/10.3767/persoonia.2022.49.08Fungal Planet description sheets: 1182-1283
Crous, P. W., Cowan, D. A., Maggs-Kolling, G., Yilmaz, N., Thangavel, R., Wingfield, M. J., Noordeloos, M. E., Dima, B., Brandrud, T. E., Jansen, G. M., Morozova, O. V., Vila, J., Shivas, R. G., Tan, Y. P., Bishop-Hurley, S., Lacey, E., Marney, T. S., Larsson, E., Le Floch, G., ..., Groenewald, J. Z.. 2021. "Fungal Planet description sheets: 1182-1283." Persoonia. 46, pp. 313-528. https://doi.org/10.3767/persoonia.2021.46.11Seven new Serendipita species associated with Australian terrestrial orchids
Oktalira, Fitria T., May, Tom W., Dearnaley, John D. W. and Linde, Celeste C.. 2021. "Seven new Serendipita species associated with Australian terrestrial orchids." Mycologia. 113 (5), pp. 968-987. https://doi.org/10.1080/00275514.2021.1919848![](/~234/dnn/default-thumbnail.png)