Immunological response to parenteral vaccination with recombinant hepatitis B virus surface antigen virus-like particles expressing Helicobacter pylori KatA epitopes in a murine H. pylori challenge model
Article
Article Title | Immunological response to parenteral vaccination with recombinant hepatitis B virus surface antigen virus-like particles expressing Helicobacter pylori KatA epitopes in a murine H. pylori challenge model |
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ERA Journal ID | 15550 |
Article Category | Article |
Authors | Kotiw, Michael, Johnson, Megan, Pandey, Manisha, Fry, Scott, Hazell, Stuart L., Netter, Hans J., Good, Michael F. and Olive, Colleen |
Journal Title | Clinical and Vaccine Immunology |
Journal Citation | 19 (2), pp. 268-276 |
Number of Pages | 9 |
Year | 2012 |
Place of Publication | Washington, DC. United States |
ISSN | 1071-412X |
1556-679X | |
1556-6811 | |
Digital Object Identifier (DOI) | https://doi.org/10.1128/CVI.05295-11 |
Web Address (URL) | http://cdli.asm.org/content/19/2/268 |
Abstract | Virus-like particles (VLPs) based on the small envelope protein of hepatitis B virus (HBsAg-S) are immunogenic at the B- and T-cell level. In this study, we inserted overlapping sequences encoding the carboxy terminus of the Helicobacter pylori katA gene product into HBsAg-S. The HBsAg-S–KatA fusion proteins were able to assemble into secretion-competent VLPs (VLP-KatA). The VLP-KatA proteins were able to induce KatA-specific antibodies in immunized mice. The mean total IgG antibody titers 41 days post-primary immunization with VLP-KatA (2.3 × 103) were significantly greater (P < 0.05) than those observed for vaccination with VLP alone (5.2 × 102). Measurement of IgG isotypes revealed responses to both IgG1 and IgG2a (mean titers, 9.0 × 104 and 2.6 × 104, respectively), with the IgG2a response to vaccination with VLP-KatA being significantly higher than that for mice immunized with KatA alone (P < 0.05). Following challenge of mice with H. pylori, a significantly reduced bacterial load in the gastric mucosa was observed (P < 0.05). This is the first report describing the use of VLPs as a delivery vehicle for H. pylori antigens. |
Keywords | aluminum hydroxide; bacterial protein; epitope; hepatitis B surface antigen; hybrid protein; immunoglobulin G1 antibody; immunoglobulin G2a antibody; KatA protein; recombinant Hepatitis B virus surface antigen Helicobacter pylori KatA fusion protein virus like particle vaccine; unclassified drug; virus like particle vaccine |
ANZSRC Field of Research 2020 | 320402. Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies) |
320705. Medical virology | |
320209. Gastroenterology and hepatology | |
Public Notes | Published ahead of print 28 December 2011 |
Byline Affiliations | Centre for Systems Biology |
Griffith University | |
University of Queensland | |
Monash University | |
Queensland Institute of Medical Research, Australia | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q1634/immunological-response-to-parenteral-vaccination-with-recombinant-hepatitis-b-virus-surface-antigen-virus-like-particles-expressing-helicobacter-pylori-kata-epitopes-in-a-murine-h-pylori-challenge
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