Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice

Article

Chen, Austen Y., Fry, Scott Robert, Forbes-Faulkner, Judy, Daggard, Grant and Mukkur, T. K. S.. 2006. "Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice." Veterinary Microbiology. 114 (3-4), pp. 252-259. https://doi.org/10.1016/j.vetmic.2005.12.009
Article Title

Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice

ERA Journal ID5560
Article CategoryArticle
AuthorsChen, Austen Y. (Author), Fry, Scott Robert (Author), Forbes-Faulkner, Judy (Author), Daggard, Grant (Author) and Mukkur, T. K. S. (Author)
Journal TitleVeterinary Microbiology
Journal Citation114 (3-4), pp. 252-259
Number of Pages8
Year2006
Place of PublicationNetherlands
ISSN0378-1135
1873-2542
Digital Object Identifier (DOI)https://doi.org/10.1016/j.vetmic.2005.12.009
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0378113505004840
Abstract

The Mycoplasma hyopneumoniae ribonucleotide reductase R2 subunit (NrdF) gene fragment was cloned into eukaryotic and prokaryotic expression vectors and its immunogenicity evaluated in mice immunized orally with attenuated Salmonella typhimurium aroA CS332 harboring either of the recombinant expression plasmids. We found that NrdF is highly conserved among M. hyopneumoniae strains. The immunogenicity of NrdF was examined by analyzing antibody responses in sera and lung washes, and the cell-mediated immune (CMI) response was assessed by determining the INF-γ level produced by splenocytes upon in vitro stimulation with NrdF antigen. S. typhimurium expressing NrdF encoded by the prokaryotic expression plasmid (pTrcNrdF) failed to elicit an NrdF-specific serum or secretory antibody response, and IFN-γ was not produced. Similarly, S. typhimurium carrying the eukaryotic recombinant plasmid encoding NrdF (pcNrdF) did not induce a serum or secretory antibody response, but did elicit significant NrdF-specific IFN-γ production, indicating induction of a CMI response. However, analysis of immune responses against the live vector S. typhimurium aroA CS332 showed a serum IgG response but no mucosal IgA response in spite of its efficient invasiveness in vitro. In the present study we show that the DNA vaccine encoding the M. hyopneumoniae antigen delivered orally via a live attenuated S. typhimurium aroA can induce a cell-mediated immune response. We also indicate that different live bacterial vaccine carriers may have an influence on the type of the immune response induced.

Keywordsimmunogenicity; mycoplasma hyopneumoniae; NrdF antigen; oral immunization
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 2020320402. Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies)
320701. Medical bacteriology
320603. Medical molecular engineering of nucleic acids and proteins
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Byline AffiliationsDepartment of Biological and Physical Sciences
Department of Primary Industries, Queensland
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https://research.usq.edu.au/item/9zwvv/comparative-immunogenicity-of-m-hyopneumoniae-nrdf-encoded-in-different-expression-systems-delivered-orally-via-attenuated-s-typhimurium-aroa-in-mice

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