Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis

Patent

Farrell, David John, Daggard, Grant Edward and Mukkur, Trilochan Kanwaljit Singh. 2001. Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis. WO/2001/90405
Title

Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis

TypePatent
AuthorsFarrell, David John (Author), Daggard, Grant Edward (Author) and Mukkur, Trilochan Kanwaljit Singh (Author)
Patent/Trademark ApplicantFarrell, David John; Daggard, Grant Edward; Mukkur, Trilochan Kanwaljit Singh
Patent/Trademark NumberWO/2001/90405
Countries in Which Patented/TrademarkedAustralia
Number of Pages32
Year2001
Web Address (URL)http://patentscope.wipo.int/search/en/WO2001090405
http://patentscope.wipo.int/search/en/detail.jsf?docId=WO2001090405&recNum=6&maxRec=6&office=&prevFilter=&sortOption=Pub+Date+Desc&queryString=FP%3A%28Shared+primer+PCR%29&tab=PCT+Biblio
Abstract

A method of detecting Bordetella pertussis and Bordetella parapertussis infection is provided. The method includes amplification of a Bordetella porin gene fragment from a sample such as a human nasopharyngeal aspirate using a Bordetella-specific primer, a Bordetella pertussis-specific primer and a Bordetella parapertussis-specific primer. The presence of the Bordetella gene fragment is then detected by hybridization to a Bordetella-specific probe. If positive, hybridization to a Bordetella pertussis-specific probe and/or a Bordetella parapertussis-specific probe is used to determine whether the porin gene fragment is indicative of Bordetella pertussis and/or Bordetella parapertussis infection. The method of the invention is designed so that errors introduced by amplification are minimized by generating amplification products of similar size and by incorporating amplification of a reference nucleic acid as an internal control.

Keywordswhooping cough; diagnosis
ANZSRC Field of Research 2020320602. Medical biotechnology diagnostics (incl. biosensors)
320103. Respiratory diseases
320211. Infectious diseases
Public Notes

This publication is copyright. It may be reproduced in whole or in part for the purposes of study, research, or review, but is subject to the inclusion of an acknowledgment of the source.

Please note that this item has a Worldwide patent.

Byline AffiliationsDepartment of Biological and Physical Sciences
Institution of OriginUniversity of Southern Queensland
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