Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice
Article
Article Title | Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice |
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ERA Journal ID | 22309 |
Article Category | Article |
Authors | Chen, Austen Y. (Author), Fry, Scott R. (Author), Daggard, Grant E. (Author) and Mukkur, Trilochan K. S. (Author) |
Journal Title | Vaccine |
Journal Citation | 26 (34), pp. 4372-4378 |
Number of Pages | 7 |
Year | 2008 |
Publisher | Elsevier |
Place of Publication | United Kingdom |
ISSN | 0264-410X |
1873-2518 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.vaccine.2008.06.005 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0264410X08007196 |
Abstract | Intramuscular immunization of mice with DNA cocktail vaccines, comprising potential protective antigens P36, P46, NrdF, and P97or P97R1 of Mycoplasma hyopneumoniae, induced strong Th1-polarized immune responses against each antigen, with only P46 eliciting a serum IgG response. Subcutaneous immunization with protein cocktail vaccines, surprisingly, induced both Th1-polarized immune response as well as antibody response whereas mice immunized with DNA cocktail vaccines followed by boosting with protein cocktail vaccines generated strong Th1-polarized and humoral immune responses. P97 was not recognized by serum antibodies from commercial bacterin-immunized mice indicating potential lack of expression of this important antigen in inactivated whole-cell vaccines. |
Keywords | antibody response; cell-mediated immunity; DNA vaccines; mycoplasma hyopneumoniae; recombinant protein vaccines |
Contains Sensitive Content | Does not contain sensitive content |
ANZSRC Field of Research 2020 | 320402. Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies) |
320701. Medical bacteriology | |
320603. Medical molecular engineering of nucleic acids and proteins | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Department of Biological and Physical Sciences |
https://research.usq.edu.au/item/9zwvw/evaluation-of-immune-response-to-recombinant-potential-protective-antigens-of-mycoplasma-hyopneumoniae-delivered-as-cocktail-dna-and-or-recombinant-protein-vaccines-in-mice
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