Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice

Article

Chen, Austen Y., Fry, Scott R., Daggard, Grant E. and Mukkur, Trilochan K. S.. 2008. "Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice." Vaccine. 26 (34), pp. 4372-4378. https://doi.org/10.1016/j.vaccine.2008.06.005
Article Title

Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice

ERA Journal ID22309
Article CategoryArticle
AuthorsChen, Austen Y. (Author), Fry, Scott R. (Author), Daggard, Grant E. (Author) and Mukkur, Trilochan K. S. (Author)
Journal TitleVaccine
Journal Citation26 (34), pp. 4372-4378
Number of Pages7
Year2008
PublisherElsevier
Place of PublicationUnited Kingdom
ISSN0264-410X
1873-2518
Digital Object Identifier (DOI)https://doi.org/10.1016/j.vaccine.2008.06.005
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0264410X08007196
Abstract

Intramuscular immunization of mice with DNA cocktail vaccines, comprising potential protective antigens P36, P46, NrdF, and P97or P97R1 of Mycoplasma hyopneumoniae, induced strong Th1-polarized immune responses against each antigen, with only P46 eliciting a serum IgG response. Subcutaneous immunization with protein cocktail vaccines, surprisingly, induced both Th1-polarized immune response as well as antibody response whereas mice immunized with DNA cocktail vaccines followed by boosting with protein cocktail vaccines generated strong Th1-polarized and humoral immune responses. P97 was not recognized by serum antibodies from commercial bacterin-immunized mice indicating potential lack of expression of this important antigen in inactivated whole-cell vaccines.

Keywordsantibody response; cell-mediated immunity; DNA vaccines; mycoplasma hyopneumoniae; recombinant protein vaccines
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 2020320402. Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies)
320701. Medical bacteriology
320603. Medical molecular engineering of nucleic acids and proteins
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Byline AffiliationsDepartment of Biological and Physical Sciences
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