Preparation of total RNA from a very small wheat embryo suitable for differential display
Article
Chen, Yihua, Weining, Song and Daggard, Grant. 2003. "Preparation of total RNA from a very small wheat embryo suitable for differential display
." Annals of Applied Biology. 143 (2), pp. 261-264. https://doi.org/10.1111/j.1744-7348.2003.tb00293.x
| Article Title | Preparation of total RNA from a very small wheat embryo suitable for differential display |
|---|---|
| ERA Journal ID | 5176 |
| Article Category | Article |
| Authors | Chen, Yihua, Weining, Song and Daggard, Grant |
| Journal Title | Annals of Applied Biology |
| Journal Citation | 143 (2), pp. 261-264 |
| Number of Pages | 4 |
| Year | 2003 |
| Publisher | John Wiley & Sons |
| Place of Publication | United Kingdom |
| ISSN | 0003-4746 |
| 1744-7348 | |
| Digital Object Identifier (DOI) | https://doi.org/10.1111/j.1744-7348.2003.tb00293.x |
| Web Address (URL) | https://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.2003.tb00293.x |
| Abstract | A simple method was developed for extracting total RNA from a single mature wheat seed embryo, which is very small and hard. Guanidine thiocyanate and chloroform were employed and the grinding of the samples was performed in a microcentrifuge tube with a plastic pestle. A "jacket" of liquid nitrogen and simplified procedures were applied to ensure the thorough grinding of the embryo tissue and to minimise the loss of samples. These measures substantially increased the recovery of total RNA in the extraction process. Reliable differential display was successfully achieved with the total RNA after DNase treatment and reverse transcription. This method makes it viable to study gene expression and gene regulation in a single wheat seed embryo. It may also give researchers the ability to analyse mRNAs in tissues or organs which were previously too small for RNA isolation using conventional procedures. |
| Keywords | Differential display; Reverse transcription; Single wheat embryo; Total RNA |
| Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
| Byline Affiliations | University of Southern Queensland |
| Department of Primary Industries, Queensland |
Permalink -
https://research.usq.edu.au/item/yy717/preparation-of-total-rna-from-a-very-small-wheat-embryo-suitable-for-differential-display
37
total views0
total downloads4
views this month0
downloads this month
Export as
Related outputs
Construction and preliminary immunobiological characterization of a novel, non-reverting, intranasal live attenuated whooping cough vaccine candidate
Cornford-Nairns, R., Daggard, G. and Mukkur, T.. 2012. "Construction and preliminary immunobiological characterization of a novel, non-reverting, intranasal live attenuated whooping cough vaccine candidate." Journal of Microbiology and Biotechnology. 22 (6), pp. 856-865. https://doi.org/10.4014/jmb.1108.08003Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis
Farrell, David John, Daggard, Grant Edward and Mukkur, Trilochan Kanwaljit Singh. 2001. Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis. WO/2001/90405Defective entities and uses therefor
Cornford-Nairn, Renee, Daggard, Grant Edward, Mukkur, Trilochan Kanwaljit Singh and Rossetti, Tony Robert. 2008. Defective entities and uses therefor. AU2002357406Parenteral immunization of mice with a genetically inactivated pertussis toxin DNA vaccine induces cell-mediated immunity and protection
Fry, Scott R., Chen, Austen Y., Daggard, Grant and Mukkur, Trilochan K. S.. 2008. "Parenteral immunization of mice with a genetically inactivated pertussis toxin DNA vaccine induces cell-mediated immunity and protection." Journal of Medical Microbiology. 57 (1), pp. 28-35. https://doi.org/10.1099/jmm.0.47527-0Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice
Chen, Austen Y., Fry, Scott R., Daggard, Grant E. and Mukkur, Trilochan K. S.. 2008. "Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice." Vaccine. 26 (34), pp. 4372-4378. https://doi.org/10.1016/j.vaccine.2008.06.005Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice
Chen, Austen Y., Fry, Scott Robert, Forbes-Faulkner, Judy, Daggard, Grant and Mukkur, T. K. S.. 2006. "Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice." Veterinary Microbiology. 114 (3-4), pp. 252-259. https://doi.org/10.1016/j.vetmic.2005.12.009Anchor primer associated problems in differential display reverse transcription polymerase chain reaction
Chen, Yihua, Wang, Bin, Weining, Song and Daggard, Grant. 2004. "Anchor primer associated problems in differential display reverse transcription polymerase chain reaction." Analytical Biochemistry. 329 (1), pp. 145-147. https://doi.org/10.1016/j.ab.2004.02.030Agrobacterium tumefaciens-mediated transformation of wheat using a superbinary vector and a polyamine-supplemented regeneration medium
Khanna, Harjeet and Daggard, Grant. 2003. "Agrobacterium tumefaciens-mediated transformation of wheat using a superbinary vector and a polyamine-supplemented regeneration medium." Plant Cell Reports. 21 (5), pp. 429-436. https://doi.org/10.1007/s00299-002-0529-xRobustness analysis of diversified ensemble decision tree algorithms for microarray data classification
Hu, Hong, Li, Jiuyong, Wang, Hua, Daggard, Grant and Wang, Li-Zhen. 2008. "Robustness analysis of diversified ensemble decision tree algorithms for microarray data classification." ICMLC 2008: 7th International Conference on Machine Learning and Cybernetics. Kunming, China 12 - 15 Jul 2008 United States. https://doi.org/10.1109/ICMLC.2008.4620389A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci
Zhang, G. W., Kotiw, M. and Daggard, G.. 2002. "A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci." Letters in Applied Microbiology. 35 (3), pp. 247-250. https://doi.org/10.1046/j.1472-765X.2002.01177.xLate-onset and recurrent neonatal group B streptococcal disease associated with breast milk transmission
Kotiw, Michael, Zhang, Gwang W., Daggard, Grant, Reiss-Levy, Elizabeth, Tapsall, John W. and Numa, Andrew. 2003. "Late-onset and recurrent neonatal group B streptococcal disease associated with breast milk transmission." Pediatric and Developmental Pathology. 6 (3), pp. 251-256. https://doi.org/10.1007/s10024-001-0276-yTargeted expression of redesigned and codon optimised synthetic gene leads to recyrstallisation inhibition and reduced electrolyte leakage in spring wheat at sub-zero temperatures
Khanna, Harjeet K. and Daggard, Grant E.. 2006. "Targeted expression of redesigned and codon optimised synthetic gene leads to recyrstallisation inhibition and reduced electrolyte leakage in spring wheat at sub-zero temperatures." Plant Cell Reports. 25 (12), pp. 1336-1346. https://doi.org/10.1007/s00299-006-0191-9Construction of three linkage maps in bread wheat, Triticum aestivum
Chalmers, K. J., Campbell, A. W., Kretschmer, J., Karakousis, A., Henschke, P. H., Pierens, S., Harker, N., Pallotta, M., Cornish, G. B., Sharifiou, M. R., Rampling, L. R., McLauchlan, A., Daggard, G., Sharp, P. J., Holton, T. A., Sutherland, M. W., Appels, R. and Langridge, P.. 2001. "Construction of three linkage maps in bread wheat, Triticum aestivum." Australian Journal of Agricultural Research. 52 (12), pp. 1089-1119. https://doi.org/10.1071/AR01081Curation of wheat maps to improve map accuracy and QTL detection
Lehmensiek, A., Eckermann, P. J., Verbyla, A. P., Appels, R., Sutherland, M. W. and Daggard, G. E.. 2005. "Curation of wheat maps to improve map accuracy and QTL detection." Australian Journal of Agricultural Research. 56 (12), pp. 1347-1354. https://doi.org/10.1071/AR05126QTLs for black-point resistance in wheat and the identification of potential markers for use in breeding programmes
Lehmensiek, A., Campbell, A. W., Williamson, P. M., Michalowitz, M., Sutherland, M. W. and Daggard, G. E.. 2004. "QTLs for black-point resistance in wheat and the identification of potential markers for use in breeding programmes." Plant Breeding. 123 (5), pp. 410-416. https://doi.org/10.1111/j.1439-0523.2004.01013.xAllelopathy, DIMBOA production and genetic variability in accessions of Triticum speltoides
Quader, Motiul, Daggard, Grant, Barrow, Russell A., Walker, Steven and Sutherland, Mark W.. 2001. "Allelopathy, DIMBOA production and genetic variability in accessions of Triticum speltoides." Journal of Chemical Ecology. 27 (4), pp. 747-760. https://doi.org/10.1023/A:1010354019573Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus
Dearnaley, John and Daggard, Grant. 2001. "Expression of a polygalacturonase enzyme in germinating pollen of Brassica napus." Sexual Plant Reproduction. 13 (5), pp. 265-271. https://doi.org/10.1007/s004970000062Wheat flour protein content and water absorption analysis in a doubled haploid population
Ma, Wujun, Sutherland, Mark W., Kammholz, Stephen, Banks, Phillip, Brennan, Paul, Bovill, William and Daggard, Grant. 2007. "Wheat flour protein content and water absorption analysis in a doubled haploid population." Journal of Cereal Science. 45 (3), pp. 302-308. https://doi.org/10.1016/j.jcs.2006.10.005Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopneumoniae as a mucosal vaccine in mice
Chen, Austen Y., Fry, Scott R., Forbes-Faulkner, Judy, Daggard, Grant and Mukkur, T. K. S.. 2006. "Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopneumoniae as a mucosal vaccine in mice." Journal of Medical Microbiology. 55 (7), pp. 923-929. https://doi.org/10.1099/jmm.0.46088-0Testing for frost tolerance in wheat (Triticum aestivum L.) transformed with a gene for antifreeze protein
Vickers, Joan, Khanna, Harjeet, Christopher, Jack, Frederiks, Troy, Stephen, John, Sutherland, Mark W. and Daggard, Grant. 2006. "Testing for frost tolerance in wheat (Triticum aestivum L.) transformed with a gene for antifreeze protein." Breeding for Success: Diversity in Action (2006). Christchurch, New Zealand 18 - 21 Apr 2006 New Zealand.Combined gene selection methods for microarray data analysis
Hu, Hong, Li, Jiuyong, Wang, Hua and Daggard, Grant. 2006. "Combined gene selection methods for microarray data analysis." Gabrys, Bogdan, Howlett, Robert J. and Jain, Lakhmi C. (ed.) 10th International Conference on Knowledge-Based Intelligent Information and Engineering Systems (KES 2006). Bournemouth, United Kingdom 09 - 11 Oct 2006 Germany. Springer. https://doi.org/10.1007/11892960_117Flour yield QTLs in three Australian doubled haploid wheat populations
Lehmensiek, A., Eckermann, P. J., Verbyla, A. P., Appels, R., Sutherland, M. W., Martin, D. and Daggard, G. E.. 2006. "Flour yield QTLs in three Australian doubled haploid wheat populations." Australian Journal of Agricultural Research. 57 (10), pp. 1115-1122. https://doi.org/10.1071/AR05375Finding similar patterns in microarray data
Chen, Xiangsheng, Li, Jiuyong, Daggard, Grant and Huang, Xiaodi. 2005. "Finding similar patterns in microarray data." Zhang, Shichao and Jarvis, Ray (ed.) AI 2005: Advances in artificial intelligence. Berlin, Germany. Springer. pp. 1272-1276Validation of black point QTL in wheat
Tah, P. R., Lehmensiek, A., Williamson, P. M., Michalowitz, M., Done, A. and Daggard, G. E.. 2008. "Validation of black point QTL in wheat." Appels, Rudi, Eastwood, Russell, Lagudah, Peter, Mackay, Michael, McIntyre, Lynne and Sharp, Peter J. (ed.) 11th International Wheat Genetics Symposium 2008 (IWGS 2008). Brisbane, Australia 24 - 29 Aug 2008 Sydney, Australia.Identification of genetic regions associated with black point in barley
Tah, Priya R., Lehmensiek, A., Fox, Glen P., Mace, Emma, Sulman, Maria, Bloustein, Gary and Daggard, Grant E.. 2010. "Identification of genetic regions associated with black point in barley." Field Crops Research. 115 (1), pp. 61-66. https://doi.org/10.1016/j.fcr.2009.10.003The correlation between the mutation of protein kinase genes and the clinical characteristics of breast cancer progression
Birney, Genevieve, Liu, Guang B. and Daggard, Grant. 2010. "The correlation between the mutation of protein kinase genes and the clinical characteristics of breast cancer progression." ASMR Queensland Postgraduate Student Conference 2010. St Lucia, Australia 02 Jun 2010 St. Lucia, Australia.Regional frost: calibration of frost chamber to encompass all Australian conditions
Daggard, Grant and Vickers, Joan. 2009. "Regional frost: calibration of frost chamber to encompass all Australian conditions." Poulsen, David (ed.) ABTS 2009: Barley - The Grain for Gain. Sunshine Coast, Australia 13 - 16 Sep 2009 Brisbane, Australia.Rapid-cycle PCR method to detect Bordetella pertussis that fulfills all consensus recommendations for use of PCR in diagnosis of pertussis
Farrell, D. J., McKeon, M., Daggard, G., Loeffelholz, M. J., Thompson, C. J. and Mukkur, T. K. S.. 2000. "Rapid-cycle PCR method to detect Bordetella pertussis that fulfills all consensus recommendations for use of PCR in diagnosis of pertussis." Journal of Clinical Microbiology. 38 (12), pp. 4499-4502.Nested duplex PCR to detect bordetella pertussis and bordetella parapertussis and its application in diagnosis of pertussis in nonmetropolitan Southeast Queensland, Australia
Farrell, D. J., Daggard, G. and Mukkur, T. K. S.. 1999. "Nested duplex PCR to detect bordetella pertussis and bordetella parapertussis and its application in diagnosis of pertussis in nonmetropolitan Southeast Queensland, Australia." Journal of Clinical Microbiology. 37 (3), pp. 606-610.Mapping markers for priority traits in the northern grains region of Australia
Mace, E., Sutherland, Mark, Lehmensiek, Anke, Bovill, Bill, Bovill, Jessica, Collard, Bert, Daggard, Grant and Christopher, M. J.. 2006. "Mapping markers for priority traits in the northern grains region of Australia." ITMI Workshop and ACPFG Genomics Symposium (2006). Victor Harbour, Australia 27 - 31 Aug 2006A maximally diversified multiple decision tree algorithm for microarray data classification
Hu, Hong, Li, Jiuyong, Wang, Hua, Daggard, Grant and Shi, Mingren. 2006. "A maximally diversified multiple decision tree algorithm for microarray data classification." Boden, Mikael and Bailey, Timothy (ed.) Workshop on Intelligent Systems for Bioinformatics (2006). Hobart, Australia 04 Dec 2006 Sydney, Australia.A comparative study of classification methods for microarray data analysis
Hu, Hong, Li, Jiuyong, Plank, Ashley, Wang, Hua and Daggard, Grant. 2006. "A comparative study of classification methods for microarray data analysis." Christen, Peter, Kennedy, Paul J., Li, Jiuyong, Simoff, Simeon J. and Williams, Graham J. (ed.) 5th Australasian Conference on Data Mining and Analystics (AusDM 2006). Sydney, Australia 29 - 30 Nov 2006 Canberra, Australia.A metabolite-deficient mutant of Bordetella pertussis that induces secretory IgA and cell-mediated immunity in mice vaccinated by the intranasal route
Mukkur, T. K. S., Cornford-Nairn, Renee A., Rossetti, T., Kershaw, S., Fry, Scott Robert, Xu, Youhong and Daggard, Grant A.. 2005. "A metabolite-deficient mutant of Bordetella pertussis that induces secretory IgA and cell-mediated immunity in mice vaccinated by the intranasal route." Fang, Ferric C. (ed.) 105th American Society for Microbiology: General Meeting. Atlanta, Georgia 05 - 09 Jun 2005 Washington.Targetting AFLP-DNA markers to specific traits and chromosome regions
Campbell, A. W., Daggard, G., Békés, F., Pedler, A., Sutherland, M. W and Appels, R.. 2001. "Targetting AFLP-DNA markers to specific traits and chromosome regions ." Australian Journal of Agricultural Research. 52 (11-12), pp. 1153-1160. https://doi.org/10.1071/ar01028Enhanced shoot regeneration in nine Australian wheat cultivars by spermidine and water stress treatments
Khanna, Harjeet K. and Daggard, Grant E.. 2001. "Enhanced shoot regeneration in nine Australian wheat cultivars by spermidine and water stress treatments ." Australian Journal of Plant Physiology. 28 (12), pp. 1243-1247. https://doi.org/10.1071/pp01115This item comprises data from the following sources: Scopus