Comparison of ELISA for Fusarium, visual screening, and deoxynivalenol analysis of Fusarium head blight for barley field nurseries

Article

Hill, N. S., Neate, S. M., Cooper, B., Horsley, R., Schwarz, P., Dahleen, L. S., Smith, K. P., O'Donnell, K. and Reeves, J.. 2008. "Comparison of ELISA for Fusarium, visual screening, and deoxynivalenol analysis of Fusarium head blight for barley field nurseries ." Crop Science: a journal serving the international community of crop scientists. 48 (4), pp. 1389-1398. https://doi.org/10.2135/cropsci2007.05.0266
Article Title

Comparison of ELISA for Fusarium, visual screening, and deoxynivalenol analysis of Fusarium head blight for barley field nurseries

ERA Journal ID5305
Article CategoryArticle
AuthorsHill, N. S. (Author), Neate, S. M. (Author), Cooper, B. (Author), Horsley, R. (Author), Schwarz, P. (Author), Dahleen, L. S. (Author), Smith, K. P. (Author), O'Donnell, K. (Author) and Reeves, J. (Author)
Journal TitleCrop Science: a journal serving the international community of crop scientists
Journal Citation48 (4), pp. 1389-1398
Number of Pages10
Year2008
PublisherJohn Wiley & Sons
Place of PublicationMadison, WI. United States
ISSN0011-183X
1435-0653
Digital Object Identifier (DOI)https://doi.org/10.2135/cropsci2007.05.0266
Web Address (URL)http://handle.nal.usda.gov/10113/18250
Abstract

Breeding for resistance to Fusarium head blight {FHB; caused by Fusarium graminearum Schwabe [teleomorph Gibberella zeae (Schwein) Petch]} is complicated because there is no accurate method for quantifying the disease organism. Most breeders rely on visual scoring for FHB and deoxynivalenol (DON) analysis to assess disease severity, but both DON and visual scoring are subject to error. The objective of this study was to compare a Fusarium-specific quantifiable enzyme-linked immunosorbent assay (ELISA) to DON and visual assessment of FHB. A doubled-haploid mapping population was grown in two environments and breeding lines in the North American Barley Scab Evaluation Nursery (NABSEN) were grown at four locations. Both experiments used a randomized complete block design and were analyzed for Fusarium by ELISA, DON, and visually scored for FHB. ELISA values for the doubled-haploid lines were consistent over years, and lines that were low in ELISA were also low in DON. Broad sense heritability was greater for ELISA (0.48) than DON (0.19) or visual scores of disease (0.29) in the NABSEN study. Numbers of locations and replications necessary to screen for disease were calculated for ELISA, DON, and FHB. ELISA required one-third to one-fourth as many replications and locations as did DON or FHB. Temperature and osmotic potential had little effect on mycelial antigen used for ELISA in vitro, but both affected DON. ELISA is a practical alternative to combined visual scores and DON analysis for breeders interested in improving FHB resistance.

KeywordsFusarium; Gibberella zeae; Hordeum; barley; scab diseases; enzyme-linked immunosorbent assay; disease diagnosis; diagnostic techniques; deoxynivalenol; breeding lines; disease resistance; genetic resistance; chromosome mapping
ANZSRC Field of Research 2020310508. Genome structure and regulation
310705. Mycology
300406. Crop and pasture improvement (incl. selection and breeding)
Public Notes

Copyright © 2008 Crop Science Society of America. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Published version deposited in accordance with the copyright policy of the publisher.

Byline AffiliationsUniversity of Georgia, United States
North Dakota State University, United States
Busch Agricultural Resources, United States
Department of Agriculture, United States
University of Minnesota, United States
Institution of OriginUniversity of Southern Queensland
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