Genome-wide association study of spot form of net blotch resistance in the upper Midwest barley breeding programs
Article
Article Title | Genome-wide association study of spot form of net blotch resistance in the upper Midwest barley breeding programs |
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ERA Journal ID | 2639 |
Article Category | Article |
Authors | Burlakoti, R. R. (Author), Gyawali, S. (Author), Chao, S. (Author), Smith, K. P. (Author), Horsley, R. D. (Author), Cooper, B. (Author), Muehlbauer, G. J. (Author) and Neate, S. M. (Author) |
Journal Title | Phytopathology: International Journal of the American Phytopathological Society |
Journal Citation | 107 (1), pp. 100-108 |
Number of Pages | 9 |
Year | 2017 |
Publisher | American Phytopathological Society |
Place of Publication | United States |
ISSN | 0031-949X |
1943-7684 | |
Digital Object Identifier (DOI) | https://doi.org/10.1094/PHYTO-03-16-0136-R |
Web Address (URL) | https://apsjournals.apsnet.org/doi/10.1094/PHYTO-03-16-0136-R |
Abstract | Pyrenophora teres f. maculata, the causal agent of spot form of net blotch (SFNB), is an emerging pathogen of barley in the United States and Australia. Compared with net form of net blotch (NFNB), less is known in the U.S. Upper Midwest barley breeding programs about host resistance and quantitative trait loci (QTL) associated with SFNB in breeding lines. The main objective of this study was to identify QTL associated with SFNB resistance in the Upper Midwest two-rowed and six-rowed barley breeding programs using a genome-wide association study approach. A total of 376 breeding lines of barley were evaluated for SFNB resistance at the seedling stage in the greenhouse in Fargo in 2009. The lines were genotyped with 3,072 single nucleotide polymorphism (SNP) markers. Phenotypic evaluation showed a wide range of variability among populations from the four breeding programs and the two barley-row types. The two-rowed barley lines were more susceptible to SFNB than the six-rowed lines. Continuous distributions of SFNB severity indicate the quantitative nature of SFNB resistance. The mixed linear model (MLM) analysis, which included both population structure and kinship matrices, was used to identify significant SNP-SFNB associations. Principal component analysis was used to control false marker-trait association. The linkage disequilibrium (LD) estimates varied among chromosomes (10 to 20 cM). The MLM analysis identified 10 potential QTL in barley: SFNB-2H-8-10, SFNB-2H-38.03, SFNB-3H-58.64, SFNB-3H-78.53, SFNB-3H-91.88, SFNB-3H-117.1, SFNB-5H-155.3, SFNB-6H-5.4, SFNB-6H-33.74, and SFNB-7H-34.82. Among them, four QTL (SFNB-2H-8-10, SFNB-2H-38.03 SFNB-3H-78.53, and SFNB-3H-117.1) have not previously been published. Identification of SFNB resistant lines and QTL associated with SFNB resistance in this study will be useful in the development of barley genotypes with better SFNB resistance. |
Keywords | association mapping, Hordeum vulgare |
ANZSRC Field of Research 2020 | 300406. Crop and pasture improvement (incl. selection and breeding) |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | North Dakota State University, United States |
Department of Agriculture, United States | |
University of Minnesota, United States | |
Busch Agricultural Resources, United States | |
Centre for Crop Health | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q3y55/genome-wide-association-study-of-spot-form-of-net-blotch-resistance-in-the-upper-midwest-barley-breeding-programs
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