A single backcross effectively eliminates agronomic and quality alterations caused by somaclonal variation in transgenic barley

Article


Bregitzer, P., Dahleen, Lynn S., Neate, Stephen, Schwarz, Paul and Manoharan, Muthusamy. 2008. "A single backcross effectively eliminates agronomic and quality alterations caused by somaclonal variation in transgenic barley ." Crop Science: a journal serving the international community of crop scientists. 48 (2), pp. 471-479. https://doi.org/10.2135/cropsci2007.06.0370
Article Title

A single backcross effectively eliminates agronomic and quality alterations caused by somaclonal variation in transgenic barley

ERA Journal ID5305
Article CategoryArticle
AuthorsBregitzer, P. (Author), Dahleen, Lynn S. (Author), Neate, Stephen (Author), Schwarz, Paul (Author) and Manoharan, Muthusamy (Author)
Journal TitleCrop Science: a journal serving the international community of crop scientists
Journal Citation48 (2), pp. 471-479
Number of Pages9
Year2008
PublisherJohn Wiley & Sons
Place of PublicationMadison, WI. United States
ISSN0011-183X
1435-0653
Digital Object Identifier (DOI)https://doi.org/10.2135/cropsci2007.06.0370
Web Address (URL)http://naldc.nal.usda.gov/naldc/download.xhtml?id=17571&content=PDF
Abstract

Transgenic crops have proven commercial utility but are created using processes known to produce undesirable variability known as somaclonal variation. This reduces the utility of transgenic germplasm to the plant breeder and complicates assessments of transgene-encoded phenotypes. Backcrossing transgenes into a wild-type genome is one solution, but producing near-isogenic lines requires a lengthy and resource-intensive process of multiple crosses. However, an abbreviated breeding scheme involving a single backcross to the wild-type parent used to produce a transgenic line, which would replace 75% of the variant alleles, should produce transgenic lines with improved performance. Comparisons were made of 'Conlon' barley (Hordeum vulgare L.), primary transgenic lines derived from Conlon, and lines derived from single backcrosses of primary transgenic lines to Conlon. The primary transgenic lines were different from Conlon for many agronomic and malting characteristics. Most of the backcross-derived lines did not differ significantly from Conlon for most agronomic characteristics. The backcross-derived lines were also similar to Conlon for malting quality traits but showed more differences than for agronomic characteristics. Differences between lines encoding TRI101 versus lines encoding PDR5 suggested that PDR5 insertion or expression may have affected malting quality. It is concluded that a single backcross is an effective, rapid, and inexpensive method for creating superior transgenic lines.

KeywordsHordeum vulgare subsp vulgare; transgenesis; cross breeding; disease resistance; somaclonal variation; transgenic plants; backcrossing; germplasm; phenotype; genome; isogenic lines; alleles; malting quality
ANZSRC Field of Research 2020310506. Gene mapping
300406. Crop and pasture improvement (incl. selection and breeding)
300110. Transgenesis
Public Notes

© 2008 Crop Science Society of America. Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Published version deposited in accordance with the copyright policy of the publisher.

Byline AffiliationsDepartment of Agriculture, United States
North Dakota State University, United States
University of Arkansas, United States
Institution of OriginUniversity of Southern Queensland
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