Trichothecene profiling and population genetic analysis of Gibberella zeae from barley in North Dakota and Minnesota

Article


Burlakoti, Rishi R., Neate, Stephen M., Adhikari, Tika B., Gyawali, Sanjaya, Salas, Bacilio, Steffenson, Brian J. and Schwarz, Paul B.. 2011. "Trichothecene profiling and population genetic analysis of Gibberella zeae from barley in North Dakota and Minnesota ." Phytopathology: International Journal of the American Phytopathological Society. 101 (6), pp. 687-695. https://doi.org/10.1094/PHYTO-04-10-0101
Article Title

Trichothecene profiling and population genetic analysis
of Gibberella zeae from barley in North Dakota and Minnesota

ERA Journal ID2639
Article CategoryArticle
AuthorsBurlakoti, Rishi R. (Author), Neate, Stephen M. (Author), Adhikari, Tika B. (Author), Gyawali, Sanjaya (Author), Salas, Bacilio (Author), Steffenson, Brian J. (Author) and Schwarz, Paul B. (Author)
Journal TitlePhytopathology: International Journal of the American Phytopathological Society
Journal Citation101 (6), pp. 687-695
Number of Pages9
Year2011
PublisherAmerican Phytopathological Society
Place of PublicationSt Paul, MN. United States
ISSN0031-949X
1943-7684
Digital Object Identifier (DOI)https://doi.org/10.1094/PHYTO-04-10-0101
Web Address (URL)http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-04-10-0101
Abstract

Gibberella zeae, the principal cause of Fusarium head blight (FHB) of barley, contaminates grains with several mycotoxins, which creates a serious problem for the malting barley industry in the United States, China, and Europe. However, limited studies have been conducted on the trichothecene profiles and population genetic structure of G. zeae isolates collected from barley in the United States. Trichothecene biosynthesis gene (TRI)-based polymerase chain reaction (PCR) assays and 10 variable number tandem repeat (VNTR) markers were used to determine the genetic diversity and compare the trichothecene profiles of an older population (n = 115 isolates) of G. zeae collected in 1997 to 2000 with a newer population (n = 147 isolates) collected in 2008. Samples were from across the major barley-growing regions in North Dakota and Minnesota. The results of TRI-based PCR assays were further validated using a subset of 32 and 28 isolates of G. zeae by sequence analysis and gas chromatography, respectively. TRI-based PCR assays revealed that all the G. zeae isolates in both populations had markers for deoxynivalenol (DON), and the frequencies of isolates with a 3-acetyldeoxynivalenol (3-ADON) marker in the newer population were ≈11-fold higher than those among isolates in the older population. G. zeae populations from barley in the Midwest of the United States showed no spatial structure, and all the isolates were solidly in clade 7 of G. zeae, which is quite different from other barley-growing areas of world, where multiple species of G. zeae are commonly found in close proximity and display spatial structure. VNTR analysis showed high gene diversity (H = 0.82 to 0.83) and genotypic diversity but low linkage disequilibrium (LD = 0.02 to 0.07) in both populations. Low genetic differentiation (FST = 0.013) and high gene flow (Nm = 36.84) was observed between the two populations and among subpopulations within the same population (Nm = 12.77 to 29.97), suggesting that temporal and spatial variations had little influence on population differentiation in the Upper Midwest. Similarly, low FST (0.02) was observed between 3-ADON and 15-acetyldeoxynivalenol populations, indicating minor influence of the chemotype of G. zeae isolates on population subdivision, although there was a rapid increase in the frequencies of isolates with the 3-ADON marker in the Upper Midwest between the older collection made in 1997 to 2000 and the newer collection made in 2008. This study provides information to barley-breeding programs for their selection of isolates of G. zeae for evaluating barley genotypes for resistance to FHB and DON accumulation.

KeywordsFusarium graminearum; Hordeum vulgare; population genetics
ANZSRC Field of Research 2020310805. Plant pathology
310705. Mycology
300409. Crop and pasture protection (incl. pests, diseases and weeds)
Public Notes

© 2011 The American Phytopathological Society. Published version deposited in accordance with the copyright policy of the publisher.

Byline AffiliationsNorth Dakota State University, United States
Department of Agriculture, United States
University of Minnesota, United States
Institution of OriginUniversity of Southern Queensland
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