The extracellular matrix regulates MaeuCath1a gene expression

Article

Wanyonyi, Stephen S., Lefevre, Christophe, Sharp, Julie A. and Nicholas, Kevin R.. 2013. "The extracellular matrix regulates MaeuCath1a gene expression." Developmental and Comparative Immunology. 40 (3-4), pp. 289-299. https://doi.org/10.1016/j.dci.2013.02.010
Article Title

The extracellular matrix regulates MaeuCath1a gene expression

ERA Journal ID2866
Article CategoryArticle
AuthorsWanyonyi, Stephen S. (Author), Lefevre, Christophe (Author), Sharp, Julie A. (Author) and Nicholas, Kevin R. (Author)
Journal TitleDevelopmental and Comparative Immunology
Journal Citation40 (3-4), pp. 289-299
Number of Pages11
Year2013
Place of PublicationUnited Kingdom
ISSN0145-305X
1879-0089
Digital Object Identifier (DOI)https://doi.org/10.1016/j.dci.2013.02.010
Web Address (URL)http://www.sciencedirect.com/science/article/pii/S0145305X13000542
Abstract

We have previously shown that the gene for MaeuCath1, a cathelicidin secreted in wallaby milk is alternately spliced into two variants, MaeuCath1a and MaeuCath1b which are temporally regulated in order to provide antimicrobial protection to the newborn and stimulate mammary growth, respectively. The current study investigated the extracellular matrix (ECM) for its regulatory role in MaeuCath1 gene expression. Reverse transcription qPCR using RNA isolated from mammary epithelial cells (WallMEC) cultured on ECM showed that ECM regulates MaeuCath1a gene expression in a lactation phase-dependent manner. Luciferase reporter-based assays and in silico analysis of deletion fragments of the 2245bp sequence upstream of the translation start site identified ECM-dependent positive regulatory activity in the -709 to -15 region and repressor activity in the -919 to -710 region. Electrophoretic Gel Mobility Shift Assays (EMSA) using nuclear extract from ECM-treated WallMEC showed differential band shift in the -839 to -710 region.

Keywordsextracellular matrix; mammary epithelial cells; cathelicidin; lactation; cathelicidin promoter; gene regulation
ANZSRC Field of Research 2020320801. Cell physiology
310105. Cellular interactions (incl. adhesion, matrix, cell wall)
320803. Systems physiology
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Byline AffiliationsDeakin University
Institution of OriginUniversity of Southern Queensland
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