Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells
Article
Article Title | Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells |
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ERA Journal ID | 15481 |
Article Category | Article |
Authors | Gao, Yuan (Author), Hannan, Nicholas R. F. (Author), Wanyonyi, Stephen (Author), Konstantopolous, Nicky (Author), Pagnon, Joanne (Author), Feng, Helen C. (Author), Jowett, Jeremy B. M. (Author), Kim, Kee-Hong (Author), Walder, Ken (Author) and Collier, Greg R. (Author) |
Journal Title | Cytokine |
Journal Citation | 33 (5), pp. 246-251 |
Number of Pages | 6 |
Year | 2006 |
Place of Publication | United Kingdom |
ISSN | 1096-0023 |
1043-4666 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.cyto.2006.02.005 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S1043466606000512 |
Abstract | SEPS1 (also called selenoprotein S, SelS) plays an important role in the production of inflammatory cytokines and its expression is activated by endoplasmic reticulum (ER) stress. In this report, we have identified two binding sites for the nuclear factor kappa B in the human SEPS1 promoter. SEPS1 gene expression, protein levels and promoter activity were all increased 2-3-fold by TNF-alpha and IL-1beta in HepG2 cells. We have also confirmed that the previously proposed ER stress response element GGATTTCTCCCCCGCCACG in the SEPS1 proximate promoter is fully functional and responsive to ER stress. However, concurrent treatment of HepG2 cells with IL-1beta and ER stress produced no additive effect on SEPS1 gene expression. We conclude that SEPS1 is a new target gene of NF-kappaB. Together with our previous findings that SEPS1 may regulate cytokine production in macrophage cells, we propose a regulatory loop between cytokines and SEPS1 that plays a key role in control of the inflammatory response. |
Keywords | selenoprotein; glucose-regulated protein; ER stress; cytokine; gene expression |
Contains Sensitive Content | Does not contain sensitive content |
ANZSRC Field of Research 2020 | 321402. Clinical pharmacology and therapeutics |
321401. Basic pharmacology | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Institution of Origin | University of Southern Queensland |
Byline Affiliations | Deakin University |
Baker Heart and Diabetes Institute, Australia | |
Medical College of Wisconsin, United States | |
ChemGenex Pharmaceuticals, Australia |
https://research.usq.edu.au/item/q3x8w/activation-of-the-selenoprotein-seps1-gene-expression-by-pro-inflammatory-cytokines-in-hepg2-cells
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