Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity

Article


Wanyonyi, Stephen S., Sharp, Julie A., Khalil, Elie, Lefevre, Christophe and Nicholas, Kevin R.. 2011. "Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity." Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology. 160 (3), pp. 431-439. https://doi.org/10.1016/j.cbpa.2011.07.015
Article Title

Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity

ERA Journal ID3157
Article CategoryArticle
AuthorsWanyonyi, Stephen S. (Author), Sharp, Julie A. (Author), Khalil, Elie (Author), Lefevre, Christophe (Author) and Nicholas, Kevin R. (Author)
Journal TitleComparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology
Journal Citation160 (3), pp. 431-439
Number of Pages9
Year2011
Place of PublicationUnited States
ISSN1095-6433
1531-4332
Digital Object Identifier (DOI)https://doi.org/10.1016/j.cbpa.2011.07.015
Web Address (URL)http://www.sciencedirect.com/science/article/pii/S1095643311002340
Abstract

Cathelicidins secreted in milk may be central to autocrine feedback in the mammary gland for optimal development in addition to conferring innate immunity to both the mammary gland and the neonate. This study exploits the unique reproductive strategy of the tammar wallaby (Macro pus eugenii) model to analyse differential splicing of cathelicidin genes and to evaluate the bactericidal activity and effect of the protein on mammary epithelial cell proliferation. Two linear peptides, Con73 and Con218, derived from the heterogeneous carboxyl end of cathelicidin transcripts, MaeuCath1 and MaeuCath7 respectively, were evaluated for antimicrobial activity. Both Con73 and Con218 significantly inhibited the growth of Staphylococcus aureus, Pseudomonas aureginosa, Enterococcus faecalis and Salmonella enterica. In addition both MaeuCath1 and MaeuCath7 stimulated proliferation of primary tammar wallaby mammary epithelial cells (WallMEC). Lactation-phase specific alternate spliced transcripts were determined for MaeuCath1 showing utilisation of both antimicrobial and proliferative functions are required by the mammary gland and the suckled young. The study has shown for the first time that temporal regulation of milk cathelicidins may be crucial in antimicrobial protection of the mammary gland and suckled young and mammary cell proliferation.

Keywordscathelicidin; tammar wallaby; mammary epithelial cells; cell proliferation; antimicrobial;
ANZSRC Field of Research 2020320801. Cell physiology
320407. Innate immunity
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Byline AffiliationsDeakin University
Institution of OriginUniversity of Southern Queensland
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