Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix
Article
Wanyonyi, Stephen S., Kumar, Amit, Du Preez, Ryan, Lefevre, Christophe and Nicholas, Kevin R.. 2017. "Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix." Biochemistry and Biophysics Reports. 12, pp. 120-128. https://doi.org/10.1016/j.bbrep.2017.08.013
| Article Title | Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix |
|---|---|
| ERA Journal ID | 210190 |
| Article Category | Article |
| Authors | Wanyonyi, Stephen S., Kumar, Amit, Du Preez, Ryan, Lefevre, Christophe and Nicholas, Kevin R. |
| Journal Title | Biochemistry and Biophysics Reports |
| Journal Citation | 12, pp. 120-128 |
| Number of Pages | 9 |
| Year | 2017 |
| Place of Publication | Netherlands |
| ISSN | 2405-5808 |
| Digital Object Identifier (DOI) | https://doi.org/10.1016/j.bbrep.2017.08.013 |
| Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S2405580817300912 |
| Abstract | Background: The unique lactation strategy of the tammar wallaby (Macropus eugeni) has been invaluable in evaluating the role of lactogenic hormones and the extracellular matrix (ECM) in the local control of mammary gland function. However molecular pathways through which hormones and ECM exert their effect on wallaby mammary gland function remain unclear. This study undertakes transcriptome analysis of wallaby mammary epithelial cells (WallMEC) following treatment with mammary ECM from two distinct stages of lactation. Methods: WallMEC from MID lactation mammary glands were cultured on ECM from MID or LATE lactation and treated for 5 days with 1 μg/ml cortisol, 1 μg/ml insulin, 0.2 µg/ml prolactin, 650 pg/ml triodothyronine and 1 pg/ml estradiol to induce lactation. WallMEC RNA from triplicate ECM treatments was used to perform RNAseq. Results: ECM from MID and LATE lactation differentially regulated key genes in sugar and lipid metabolism. Seven pathways including galactose metabolism, lysosome, cell adhesion molecules (CAM), p53 signaling, the complement and coagulation and Nod-like receptor signaling pathways were only significantly responsive to ECM in the presence of hormones. The raw RNA-seq data for this project are available on the NCBI Gene Expression Omnibus (GEO) browser (accession number GSE81210). Conclusions: A potential role of ECM in regulation of the caloric content of milk, among other functions including apoptosis, cell proliferation and differentiation has been identified. General significance This study has used a non-eutherian lactation model to demonstrate the synergy between ECM and hormones in the local regulation of mammary function. |
| Keywords | Milk lipid composition |
| Contains Sensitive Content | Does not contain sensitive content |
| ANZSRC Field of Research 2020 | 310105. Cellular interactions (incl. adhesion, matrix, cell wall) |
| Funder | Deakin University |
| Byline Affiliations | University of Southern Queensland |
| Walter and Eliza Hall Institute of Medical Research, Australia | |
| Central Queensland University | |
| University of Melbourne | |
| Monash University |
Permalink -
https://research.usq.edu.au/item/w751v/transcriptome-analysis-of-mammary-epithelial-cell-gene-expression-reveals-novel-roles-of-the-extracellular-matrix
Download files
Published Version
| Transcriptome analysis of mammary epithelial cell.pdf | ||
| License: CC BY-NC-ND 4.0 | ||
| File access level: Anyone | ||
16
total views14
total downloads1
views this month1
downloads this month
Export as
Related outputs
Nanocurcumin
Du Preez, Ryan. Nanocurcumin. Toowoomba. https://doi.org/10.26192/sazr-0182Functional Foods Research Group: Stephen Wanyonyi
Wanyonyi, Stephen. Functional Foods Research Group: Stephen Wanyonyi. Toowoomba. https://doi.org/10.26192/2z6r-wx03Tropical foods as functional foods for metabolic syndrome
John, Oliver D., du Preez, Ryan, Panchal, Sunil K. and Brown, Lindsay. 2020. "Tropical foods as functional foods for metabolic syndrome." Food and Function. 11 (8), pp. 6946-6960. https://doi.org/10.1039/d0fo01133aBrown Seaweed Sargassum siliquosum as an Intervention for Diet-Induced Obesity in Male Wistar Rats
du Preez, Ryan, Magnusson, Marie, Majzoub, Marwan E., Thomas, Torsten, Praeger, Christina, Glasson, Christopher R. K., Panchal, Sunil K. and Brown, Lindsay. 2021. "Brown Seaweed Sargassum siliquosum as an Intervention for Diet-Induced Obesity in Male Wistar Rats." Nutrients. 13 (6). https://doi.org/10.3390/nu13061754Nannochloropsis oceanica as a Microalgal Food Intervention in Diet-Induced Metabolic Syndrome in Rats
du Preez, Ryan, Majzoub, Marwan E., Thomas, Torsten, Panchal, Sunil K. and Brown, Lindsay. 2021. "Nannochloropsis oceanica as a Microalgal Food Intervention in Diet-Induced Metabolic Syndrome in Rats." Nutrients. 13 (11). https://doi.org/10.3390/nu13113991Algae provide options for improved treatment of metabolic syndrome
du Preez, Ryan. 2019. Algae provide options for improved treatment of metabolic syndrome. PhD Thesis Doctor of Philosophy. University of Southern Queensland. https://doi.org/10.26192/bn0p-yb02Saskatoon Berry Amelanchier alnifolia Regulates Glucose Metabolism and Improves Cardiovascular and Liver Signs of Diet-Induced Metabolic Syndrome in Rats
du Preez, Ryan, Wanyonyi, Stephen, Mouatt, Peter, Panchal, Sunil K. and Brown, Lindsay. 2020. "Saskatoon Berry Amelanchier alnifolia Regulates Glucose Metabolism and Improves Cardiovascular and Liver Signs of Diet-Induced Metabolic Syndrome in Rats." Nutrients. 12 (4), pp. 1-16. https://doi.org/10.3390/nu12040931Carrageenans from the Red Seaweed Sarconema filiforme Attenuate Symptoms of Diet-Induced Metabolic Syndrome in Rats
du Preez, Ryan, Paul, Nicholas, Mouatt, Peter, Majzoub, Marwan E., Thomas, Torsten, Panchal, Sunil K. and Brown, Lindsay. 2020. "Carrageenans from the Red Seaweed Sarconema filiforme Attenuate Symptoms of Diet-Induced Metabolic Syndrome in Rats." Marine Drugs. 18 (2), pp. 1-26. https://doi.org/10.3390/md18020097Caulerpa lentillifera (Sea Grapes) Improves Cardiovascular and Metabolic Health of Rats with Diet-Induced Metabolic Syndrome
du Preez, Ryan, Majzoub, Marwan E., Thomas, Torsten, Panchal, Sunil K. and Brown, Lindsay. 2020. "Caulerpa lentillifera (Sea Grapes) Improves Cardiovascular and Metabolic Health of Rats with Diet-Induced Metabolic Syndrome." Metabolites. 10 (12), pp. 1-18. https://doi.org/10.3390/metabo10120500Low-dose curcumin nanoparticles normalise blood pressure in male Wistar rats with diet-induced metabolic syndrome
du Preez, Ryan, Pahl, Jessica, Arora, Meenakshi, Kumar, M. N. V. Ravi, Brown, Lindsay and Panchal, Sunil K.. 2019. "Low-dose curcumin nanoparticles normalise blood pressure in male Wistar rats with diet-induced metabolic syndrome." Nutrients. 11 (7), pp. 1-15. https://doi.org/10.3390/nu11071542Linseed components are more effective than whole linseed in reversing diet-induced metabolic syndrome in rats
Shafie, Siti Raihanah, Wanyonyi, Stephen, Panchal, Sunil K. and Brown, Lindsay. 2019. "Linseed components are more effective than whole linseed in reversing diet-induced metabolic syndrome in rats." Nutrients. 11 (1677), pp. 1-16. https://doi.org/10.3390/nu11071677Achacha (Garcinia humilis) Rind Improves Cardiovascular Function in Rats with Diet-Induced Metabolic Syndrome
John, Oliver D., Wanyonyi, Stephen, Mouatt, Peter, Panchal, Sunil K. and Brown, Lindsay. 2018. "Achacha (Garcinia humilis) Rind Improves Cardiovascular Function in Rats with Diet-Induced Metabolic Syndrome." Nutrients. 10 (10), pp. 1-15. https://doi.org/10.3390/nu10101425Kappaphycus alvarezii as a food supplement prevents diet-induced metabolic syndrome in rats
Wanyonyi, Stephen, du Preez, Ryan, Brown, Lindsay, Paul, Nicholas A. and Panchal, Sunil K.. 2017. "Kappaphycus alvarezii as a food supplement prevents diet-induced metabolic syndrome in rats." Nutrients. 9 (11). https://doi.org/10.3390/nu9111261The comparative genomics of monotremes, marsupials, and pinnipeds: models to examine the functions of milk proteins
Sharp, Julie A., Watt, Ashalyn, Bisana, Swathi, Modepalli, Vengama, Wanyonyi, Stephen, Kumar, Amit, Kwek, Joly, Collins, Rod, Lefevre, Christopher and Nicholas, Kevin R.. 2014. "The comparative genomics of monotremes, marsupials, and pinnipeds: models to examine the functions of milk proteins ." Singh, Harjinder, Boland, Mike and Thompson, Abby (ed.) Milk proteins: from expression to food. Amsterdam, The Netherlands. Elsevier. pp. 75-112Inhibition of inosine monophosphate dehydrogenase reduces adipogenesis and diet-induced obesity
Su, Hua, Gunter, Jennifer H., de Vries, Melissa, Connor, Tim, Wanyonyi, Stephen, Newell, Felicity S., Segal, David, Molero, Juan Carlos, Reizes, Ofer, Prins, Johannes B., Hutley, Louise J., Walder, Ken and Whitehead, Jonathan P.. 2009. "Inhibition of inosine monophosphate dehydrogenase reduces adipogenesis and diet-induced obesity." Biochemical and Biophysical Research Communications. 386 (2), pp. 351-355. https://doi.org/10.1016/j.bbrc.2009.06.040Selenium, vanadium, and chromium as micronutrients to improve metabolic syndrome
Panchal, Sunil K., Wanyonyi, Stephen and Brown, Lindsay. 2017. "Selenium, vanadium, and chromium as micronutrients to improve metabolic syndrome." Current Hypertension Reports. 19 (3), pp. 10-20. https://doi.org/10.1007/s11906-017-0701-xA gene expression signature for insulin resistance
Konstantopoulos, Nicky, Foletta, Victoria C., Segal, David H., Shields, Katherine A., Sanigorski, Andrew, Windmill, Kelly, Swinton, Courtney, Connor, Tim, Wanyonyi, Stephen, Dyer, Thomas D., Fahey, Richard P., Watt, Rose A., Curran, Joanne E., Molero, Juan C., Krippner, Guy, Collier, Greg R., James, David E., Blangero, John, Jowett, Jeremy B. and Walder, Ken R.. 2011. "A gene expression signature for insulin resistance." Physiological Genomics. 43 (3), pp. 110-120. https://doi.org/10.1152/physiolgenomics.00115.2010Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity
Wanyonyi, Stephen S., Sharp, Julie A., Khalil, Elie, Lefevre, Christophe and Nicholas, Kevin R.. 2011. "Tammar wallaby mammary cathelicidins are differentially expressed during lactation and exhibit antimicrobial and cell proliferative activity." Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology. 160 (3), pp. 431-439. https://doi.org/10.1016/j.cbpa.2011.07.015Methazolamide is a new hepatic insulin sensitizer that lowers blood glucose in vivo
Konstantopoulos, Nicky, Molero, Juan C., McGee, Sean L., Spolding, Briana, Connor, Tim, de Vries, Melissa, Wanyonyi, Stephen, Fahey, Richard, Morrison, Shona, Swinton, Courtney, Jones, Sharon, Cooper, Adrian, Garcia-Guerra, Lucia, Foletta, Victoria C., Krippner, Guy, Andrikopoulos, Sofianos and Walder, Ken R.. 2012. "Methazolamide is a new hepatic insulin sensitizer that lowers blood glucose in vivo." Diabetes. 61 (8), pp. 2146-2154. https://doi.org/10.2337/db11-0578The tammar wallaby: a model system to examine domain-specific delivery of milk protein bioactives
Nicholas, Kevin, Sharp, Julie, Watt, Ashalyn, Wanyonyi, Stephen, Crowley, Tamsyn, Gillespie, Meagan and Lefevre, Christophe. 2012. "The tammar wallaby: a model system to examine domain-specific delivery of milk protein bioactives." Seminars in Cell and Developmental Biology. 23 (5), pp. 547-556. https://doi.org/10.1016/j.semcdb.2012.03.016The extracellular matrix regulates MaeuCath1a gene expression
Wanyonyi, Stephen S., Lefevre, Christophe, Sharp, Julie A. and Nicholas, Kevin R.. 2013. "The extracellular matrix regulates MaeuCath1a gene expression." Developmental and Comparative Immunology. 40 (3-4), pp. 289-299. https://doi.org/10.1016/j.dci.2013.02.010The extracellular matrix locally regulates asynchronous concurrent lactation in tammar wallaby (Macropus eugenii)
Wanyonyi, Stephen S., Lefevre, Christophe, Sharp, Julie A. and Nicholas, Kevin R.. 2013. "The extracellular matrix locally regulates asynchronous concurrent lactation in tammar wallaby (Macropus eugenii)." Matrix Biology. 32 (6), pp. 342-351. https://doi.org/10.1016/j.matbio.2013.02.001The tammar wallaby: a marsupial model to examine the timed delivery and role of bioactives in milk
Sharp, Julie A., Wanyonyi, Stephen, Modepalli, Vengama, Watt, Ashalyn, Kuruppath, Sanjana, Hinds, Lyn A., Kumar, Amit, Abud, Helen E., Lefevre, Christophe and Nicholas, Kevin R.. 2017. "The tammar wallaby: a marsupial model to examine the timed delivery and role of bioactives in milk." General and Comparative Endocrinology. 244, pp. 164-177. https://doi.org/10.1016/j.ygcen.2016.08.007Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells
Gao, Yuan, Hannan, Nicholas R. F., Wanyonyi, Stephen, Konstantopolous, Nicky, Pagnon, Joanne, Feng, Helen C., Jowett, Jeremy B. M., Kim, Kee-Hong, Walder, Ken and Collier, Greg R.. 2006. "Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells." Cytokine. 33 (5), pp. 246-251. https://doi.org/10.1016/j.cyto.2006.02.005This item comprises data from the following sources: Scopus