Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix

Article


Wanyonyi, Stephen S., Kumar, Amit, Du Preez, Ryan, Lefevre, Christophe and Nicholas, Kevin R.. 2017. "Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix." Biochemistry and Biophysics Reports. 12, pp. 120-128. https://doi.org/10.1016/j.bbrep.2017.08.013
Article Title

Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix

ERA Journal ID210190
Article CategoryArticle
AuthorsWanyonyi, Stephen S., Kumar, Amit, Du Preez, Ryan, Lefevre, Christophe and Nicholas, Kevin R.
Journal TitleBiochemistry and Biophysics Reports
Journal Citation12, pp. 120-128
Number of Pages9
Year2017
Place of PublicationNetherlands
ISSN2405-5808
Digital Object Identifier (DOI)https://doi.org/10.1016/j.bbrep.2017.08.013
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S2405580817300912
Abstract

Background: The unique lactation strategy of the tammar wallaby (Macropus eugeni) has been invaluable in evaluating the role of lactogenic hormones and the extracellular matrix (ECM) in the local control of mammary gland function. However molecular pathways through which hormones and ECM exert their effect on wallaby mammary gland function remain unclear. This study undertakes transcriptome analysis of wallaby mammary epithelial cells (WallMEC) following treatment with mammary ECM from two distinct stages of lactation.

Methods: WallMEC from MID lactation mammary glands were cultured on ECM from MID or LATE lactation and treated for 5 days with 1 μg/ml cortisol, 1 μg/ml insulin, 0.2 µg/ml prolactin, 650 pg/ml triodothyronine and 1 pg/ml estradiol to induce lactation. WallMEC RNA from triplicate ECM treatments was used to perform RNAseq.

Results: ECM from MID and LATE lactation differentially regulated key genes in sugar and lipid metabolism. Seven pathways including galactose metabolism, lysosome, cell adhesion molecules (CAM), p53 signaling, the complement and coagulation and Nod-like receptor signaling pathways were only significantly responsive to ECM in the presence of hormones. The raw RNA-seq data for this project are available on the NCBI Gene Expression Omnibus (GEO) browser (accession number GSE81210).

Conclusions: A potential role of ECM in regulation of the caloric content of milk, among other functions including apoptosis, cell proliferation and differentiation has been identified. General significance This study has used a non-eutherian lactation model to demonstrate the synergy between ECM and hormones in the local regulation of mammary function.

KeywordsMilk lipid composition
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 2020310105. Cellular interactions (incl. adhesion, matrix, cell wall)
FunderDeakin University
Byline AffiliationsUniversity of Southern Queensland
Walter and Eliza Hall Institute of Medical Research, Australia
Central Queensland University
University of Melbourne
Monash University
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