A Novel Approach to the Design and Analysis of Field Experiments to Study Variation in the Tolerance and Resistance of Cultivars to Root Lesion Nematodes (Pratylenchus spp.)

Article


Reeves, Karyn L., Forknall, Clayton R., Kelly, Alison M., Owen, Kirsty J., Fanning, Joshua, Hollaway, Grant J. and Loughman, Robert. 2020. "A Novel Approach to the Design and Analysis of Field Experiments to Study Variation in the Tolerance and Resistance of Cultivars to Root Lesion Nematodes (Pratylenchus spp.)." Phytopathology: International Journal of the American Phytopathological Society. 110 (10), pp. 1623-1631. https://doi.org/10.1094/PHYTO-03-20-0077-R
Article Title

A Novel Approach to the Design and Analysis of Field Experiments to Study Variation in the Tolerance and Resistance of Cultivars to Root Lesion Nematodes (Pratylenchus spp.)

ERA Journal ID2639
Article CategoryArticle
AuthorsReeves, Karyn L. (Author), Forknall, Clayton R. (Author), Kelly, Alison M. (Author), Owen, Kirsty J. (Author), Fanning, Joshua (Author), Hollaway, Grant J. (Author) and Loughman, Robert (Author)
Journal TitlePhytopathology: International Journal of the American Phytopathological Society
Journal Citation110 (10), pp. 1623-1631
Number of Pages9
Year2020
PublisherAmerican Phytopathological Society
Place of PublicationUnited States
ISSN0031-949X
1943-7684
Digital Object Identifier (DOI)https://doi.org/10.1094/PHYTO-03-20-0077-R
Web Address (URL)https://apsjournals.apsnet.org/doi/10.1094/PHYTO-03-20-0077-R
Abstract

The root lesion nematode (RLN) species Pratylenchus thornei and P. neglectus are widely distributed within cropping regions of Australia and limit grain production. Field experiments conducted to compare the performance of cultivars in the presence of RLN investigate management options for growers by identifying cultivars with resistance, by limiting nematode reproduction, and tolerance, by yielding well in the presence of nematodes. A novel experimental design approach for RLN experiments is proposed where the observed RLN density, measured before sowing, is used to condition the randomization of cultivars to field plots. This approach ensured that all cultivars were exposed to consistent ranges of RLN in order to derive valid assessments of relative cultivar tolerance and resistance. It overcame problems inherent in conventional experimental approaches which can result in biased estimates or the confounding of cultivar yield potential, tolerance and resistance. Using data from a field experiment designed using the new approach and conducted in Formartin, Australia, the analysis of tolerance and resistance was undertaken in a linear mixed model framework. Yield response curves were derived using a random regression approach and curves modelling change in RLN densities between sowing and harvest were derived using splines to model non-linearity. This approach allowed cultivar resistance to be modelled over a consistent range of pre-sowing RLN densities. Groups of cultivars sharing similar resistance levels could be identified. A comparison of slopes of yield response curves of cultivars belonging to the same resistance class identified differing tolerance levels for cultivars with equivalent exposures to both pre-sowing and post-harvest RLN densities. The proposed design and analysis approach allowed tolerance to be established independently of resistance.

Keywordsdisease control and pest management, experimental design, nematology, population biology, random regression analysis, resistance, tolerance
ANZSRC Field of Research 2020300409. Crop and pasture protection (incl. pests, diseases and weeds)
Byline AffiliationsCurtin University
Department of Agriculture and Fisheries, Queensland
Department of Agriculture, Fisheries and Forestry, Queensland
Agriculture Victoria
Department of Primary Industries and Regional Development, Western Australia
Institution of OriginUniversity of Southern Queensland
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