Fluorescence-based screening of microbial status during bioprocesses

Paper


Learmonth, Robert P.. 2005. "Fluorescence-based screening of microbial status during bioprocesses." Jameson, David M. and Wright, J. (ed.) 6th International Weber Symposium on Innovative Fluorescence Methodologies on Biochemistry & Medicine. Kauai, Hawaii 22 - 27 Jul 2005
Paper/Presentation Title

Fluorescence-based screening of microbial status during bioprocesses

Presentation TypePaper
Authors
AuthorLearmonth, Robert P.
EditorsJameson, David M. and Wright, J.
Journal or Proceedings TitleProceedings of the 6th International Weber Symposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine
Number of Pages1
Year2005
Web Address (URL) of Paperhttp://www.lfd.uci.edu/weber/symposium/2005/
Conference/Event6th International Weber Symposium on Innovative Fluorescence Methodologies on Biochemistry & Medicine
Event Details
6th International Weber Symposium on Innovative Fluorescence Methodologies on Biochemistry & Medicine
Event Date
22 to end of 27 Jul 2005
Event Location
Kauai, Hawaii
Abstract

[Abstract]: During microbial fermentations and other bioprocesses, monitoring of potential contamination,
cell viability, physiological status and performance is of prime importance. As a given bioprocess
advances, changes in population dynamics, cell physiology or process constituents will likely
have impacts that require intervention. Various off-line procedures have been employed to
estimate status of cells, for example identity, viability, stress tolerance and vitality. However the
current techniques are both protracted and labour-intensive, so are not useful for monitoring
rapidly changing processes. We are developing fluorescence-based techniques to provide
feedback on cell status in rapid timeframes that enable realistic on-line control of process
parameters. Spectroscopic approaches enable on-line analysis of cell populations using simple
and robust instrumentation. While microscopy-based approaches remain labour intensive and
must be carried out off-line, they provide important information on differential individual cell
responses and spatial relationships that may be critical to overall performance. In parallel
studies fluorescence-based approaches have been established or are under development to
determine viability and identity, respectively. The major focus of investigations described here is
assessment in whole cell systems of modulation of cell membrane fluidity, as an indicator of
adaptability and vitality. Membrane fluidity is determined by fluorescence Polarization and
Generalized Polarization of the membrane-localising probe 6-lauroyl-2-dimethylamino
naphthalene (laurdan). Fluidity modulation has been observed in relation to physiological state
(growth phase and glucose repression), temperature up shift and increased concentrations of
ethanol. Furthermore, we detected effects on cell membranes of process additives that may
potentially be toxic or membrane-active, in both yeast and bacterial systems.

KeywordsFluorescence-based screening, microbial status, bioprocesses
ANZSRC Field of Research 2020310110. Receptors and membrane biology
310603. Fermentation
320602. Medical biotechnology diagnostics (incl. biosensors)
Public Notes

Conference publication consists of only the abstracts of papers presented at the conference. Abstract only posted here. No evidence of copyright restrictions.

Byline AffiliationsDepartment of Biological and Physical Sciences
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