Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations

Article

Croney, John C., Jameson, David M. and Learmonth, Robert P.. 2001. "Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations." Biochemistry and Molecular Biology Education. 29 (2), pp. 60-65. https://doi.org/10.1016/S1470-8175(01)00019-4
Article Title

Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations

ERA Journal ID20634
Article CategoryArticle
AuthorsCroney, John C. (Author), Jameson, David M. (Author) and Learmonth, Robert P. (Author)
Journal TitleBiochemistry and Molecular Biology Education
Journal Citation29 (2), pp. 60-65
Number of Pages6
Year2001
Place of PublicationHoboken, NJ., United States
ISSN1470-8175
1539-3429
Digital Object Identifier (DOI)https://doi.org/10.1016/S1470-8175(01)00019-4
Web Address (URL)https://www.sciencedirect.com/science/article/abs/pii/S1470817501000194
Abstract

Although most biochemistry curricula include some treatment of light absorption and spectrophotometry, discussion of fluorescence spectroscopy is generally omitted. This omission is unfortunate given the increasing use of fluorescence in many fields of biochemical research. In this paper we briefly review the principles and applications of fluorescence in biochemical systems, from the viewpoint of teaching fluorescence in undergraduate curricula. Simple practical demonstrations are presented, which clearly demonstrate important concepts yet require minimal specialized equipment.

Keywordsfluorescence; teaching; education; biochemistry; undergraduate; spectroscopy
ANZSRC Field of Research 2020340399. Macromolecular and materials chemistry not elsewhere classified
390113. Science, technology and engineering curriculum and pedagogy
511099. Synchrotrons and accelerators not elsewhere classified
Public Notes

Copyright © 2001 IUBMB. Published version deposited in accordance with the copyright policy of the publisher.

Byline AffiliationsUniversity of Hawaii, United States
Department of Biological and Physical Sciences
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