Effects of supplementation with L-proline or inositol on yeast membrane fluidity and ethanol tolerance

Poster

Ishmayana, Safri, Kennedy, Ursula and Learmonth, Robert. 2011. "Effects of supplementation with L-proline or inositol on yeast membrane fluidity and ethanol tolerance." 12th Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes (MAF12). Strasbourg, France 11 - 14 Sep 2011 Strasbourg, France.
Paper/Presentation Title

Effects of supplementation with L-proline or inositol on yeast membrane fluidity and ethanol tolerance

Presentation TypePoster
AuthorsIshmayana, Safri (Author), Kennedy, Ursula (Author) and Learmonth, Robert (Author)
Journal or Proceedings TitleProceedings of the 12th Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes (MAF12)
Number of Pages1
Year2011
Place of PublicationStrasbourg, France
Web Address (URL) of Paperhttp://maf12.unistra.fr
Conference/Event12th Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes (MAF12)
Event Details
12th Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes (MAF12)
Event Date
11 to end of 14 Sep 2011
Event Location
Strasbourg, France
Abstract

Fermentation performance of the yeast Saccharomyces cerevisiae in media with high sugar concentration

Safri Ishmayana[1,2], Robert P. Learmonth[2], Ursula J. Kennedy[2]

[1]Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jln. Raya Bandung-Sumedang km. 21 Jatinangor, West Java, 45363 Indonesia
[2]Centre for System Biology, University of Southern Queensland, West St., Toowoomba, QLD 4350 Australia

Abstract

High sugar concentration is more preferred in industrial bioethanol production, as it can increase the amount of ethanol produced by the end of fermentation. However, when high sugar concentration is used in the media, yeast cells are exposed to high osmotic stress, which can affect the fermentation performance. The present experiment aimed to investigate the fermentation performance of the yeast S. cerevisiae at high sugar concentration, in poor (Yeast nitrogen base/YNB) and rich (yeast extract peptone/YEP) media. Growth parameters and sugar utilization were monitored during the fermentations. The results indicate that all three strains used had stuck fermentations, leaving 50 to 60% residual sugar. Therefore, a follow up experiment was conducted by using media of different nutritional value, including YNB with nitrogen supplementation and YEP, as well as different sugars (sucrose or glucose). Yeast cell grown in YEP had better fermentation performance indicated by higher sugar utilization. Addition of ammonium sulphate to YNB media did not change fermentation performance of the yeast cells. In YEP media, cells grown with glucose tended to maintain better viability than sucrose. Our study confirmed that nutrient availability is very important for fermentation performance. Comparison of YNB and YEP media indicates that nutritionally insufficient media are not suitable for high concentration sugar fermentation.

Keywordsfermentation performance, high sugar concentration, nutrition availability
ANZSRC Field of Research 2020310603. Fermentation
Public Notes

This poster paper was not peer-reviewed. Abstract only published.

Byline AffiliationsDepartment of Biological and Physical Sciences
Centre for Systems Biology
Institution of OriginUniversity of Southern Queensland
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