Use of fluorescence spectroscopy to differentiate yeast and bacterial cells

Article

Bhatta, H., Goldys, Ewa M. and Learmonth, Robert P.. 2006. "Use of fluorescence spectroscopy to differentiate yeast and bacterial cells." Applied Microbiology and Biotechnology. 71 (1), pp. 121-126. https://doi.org/10.1007/s00253-005-0309-y
Article Title

Use of fluorescence spectroscopy to differentiate yeast and bacterial cells

ERA Journal ID3330
Article CategoryArticle
AuthorsBhatta, H. (Author), Goldys, Ewa M. (Author) and Learmonth, Robert P. (Author)
Journal TitleApplied Microbiology and Biotechnology
Journal Citation71 (1), pp. 121-126
Number of Pages6
Year2006
Place of PublicationBerlin, Germany
ISSN0175-7598
1432-0614
Digital Object Identifier (DOI)https://doi.org/10.1007/s00253-005-0309-y
Web Address (URL)http://www.springerlink.com/content/b0146k3r2j25716n/fulltext.pdf
Abstract

This study focuses on the characterization of bacterial and yeast species through their autofluorescence spectra. Lactic acid bacteria (Lactobacillus sp.), and yeast(Saccharomyces sp.) were cultured under controlled
conditions and studied for variations in their autofluorescence, particularly in the area representative of tryptophan residues of proteins. The emission and excitation spectra clearly reveal that bacterial and yeast species can be differentiated by their intrinsic fluorescence with UV excitation. The possibility of differentiation between different strains of Saccharomyces yeast was also studied, with clear differences observed for selected strains. The study shows that fluorescence can be successfully used to differentiate between yeast and bacteria and between different yeast species, through the identification of spectroscopic fingerprints, without the need for fluorescent staining.

Keywordsfluorescence spectroscopy; bacteria; yeast; differentiation
ANZSRC Field of Research 2020340399. Macromolecular and materials chemistry not elsewhere classified
310605. Industrial microbiology (incl. biofeedstocks)
310604. Industrial biotechnology diagnostics (incl. biosensors)
Public Notes

File reproduced in accordance with the copyright policy of the publisher/author.

Byline AffiliationsMacquarie University
Centre for Systems Biology
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