Fermentation performance of the yeast Saccharomyces cerevisiae in media with high sugar concentration
Paper
Paper/Presentation Title | Fermentation performance of the yeast Saccharomyces cerevisiae in media with high sugar concentration |
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Presentation Type | Paper |
Authors | Ishmayana, Safri (Author), Learmonth, Robert P. (Author) and Kennedy, Ursula J. (Author) |
Journal or Proceedings Title | Proceedings of the 2nd International Seminar on Chemistry: Chemestry for a Better Future (ISC 2011) |
Number of Pages | 7 |
Year | 2011 |
Place of Publication | Sumedang, Indonesia |
ISBN | 9786021941317 |
Web Address (URL) of Paper | http://chemistry.unpad.ac.id/isc-2011/ |
Conference/Event | 2nd International Seminar on Chemistry: Chemestry for a Better Future (ISC 2011) |
Event Details | 2nd International Seminar on Chemistry: Chemestry for a Better Future (ISC 2011) Event Date 24 to end of 25 Nov 2011 Event Location Bandung, Indonesia |
Abstract | High sugar concentration is more preferred in industrial bioethanol production, as it can increase the amount of ethanol produced by the end of fermentation. However, when high sugar concentration is used in the media, yeast cells are exposed to high osmotic stress, which can affect the fermentation performance. The present experiment aimed to investigate the fermentation performance of the yeast S. cerevisiae at high sugar concentration, in poor (Yeast nitrogen base/YNB) and rich (yeast extract peptone/YEP) media. Growth parameters and sugar utilization were monitored during the fermentations. The results indicate that all three strains used had stuck fermentations, leaving 50 to 60% residual sugar. Therefore, a follow up experiment was conducted by using media of different nutritional value, including YNB with nitrogen supplementation and YEP, as well as different sugars (sucrose or glucose). Yeast cell grown in YEP had better fermentation performance indicated by higher sugar utilization. Addition of ammonium sulphate to YNB media did not change fermentation performance of the yeast cells. In YEP media, cells grown with glucose tended to maintain better viability than sucrose. Our study confirmed that nutrient availability is very important for fermentation performance. Comparison of YNB and YEP media indicates that nutritionally insufficient media are not suitable for high concentration sugar fermentation. |
Keywords | fermentation performance; high sugar concentration; nutrition availability |
ANZSRC Field of Research 2020 | 310603. Fermentation |
400201. Automotive combustion and fuel engineering | |
349999. Other chemical sciences not elsewhere classified | |
Public Notes | This was an invited address. No evidence of copyright restrictions preventing deposit. |
Byline Affiliations | Centre for Systems Biology |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q1205/fermentation-performance-of-the-yeast-saccharomyces-cerevisiae-in-media-with-high-sugar-concentration
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