Basics of fluorescence
Edited book (chapter)
Chapter Title | Basics of fluorescence |
---|---|
Book Chapter Category | Edited book (chapter) |
ERA Publisher ID | 3809 |
Book Title | Fluorescence applications in biotechnology and life sciences |
Authors | Learmonth, Robert P. (Author), Kable, Scott H. (Author) and Ghiggino, Kenneth P. (Author) |
Editors | Goldys, Ewa M. |
Page Range | 1-26 |
Number of Pages | 26 |
Year | 2009 |
Publisher | Wiley-Blackwell |
Place of Publication | Hoboken, NJ, USA |
ISBN | 9780470083703 |
Web Address (URL) | http://media.johnwiley.com.au/product_data/excerpt/00/04700837/0470083700.pdf |
Abstract | The objective of this chapter is to provide a simple understanding of molecular spectroscopy, with a focus on concepts and techniques relevant to microscopy. The inherently quantum mechanical concepts of phenomena, including energy levels, radiative and nonradiative processes, and quantum yields, will be developed from a nonmathematical framework that is often used by experts in spectroscopy when discussing general principles. This simplified framework is developed into a practical and useful understanding of optical spectroscopy aimed at nonspecialists. This groundwork supports the more detailed discussion of complex techniques in the following chapters. |
Keywords | fluorescence; light; absorption; emission; non-radiative decay; spectroscopy; microscopy |
ANZSRC Field of Research 2020 | 310199. Biochemistry and cell biology not elsewhere classified |
510302. Classical and physical optics | |
510203. Nonlinear optics and spectroscopy | |
310699. Industrial biotechnology not elsewhere classified | |
Public Notes | Chapter 1. This publication arose from work and activities supported by the Australian Research Council and National Health and Medial Research Council Research Network grant RN460002. |
Byline Affiliations | Department of Biological and Physical Sciences |
University of Sydney | |
University of Melbourne |
https://research.usq.edu.au/item/9z40z/basics-of-fluorescence
1972
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