Laser-based fluorescence microscopy

Article

Learmonth, Robert P.. 2002. "Laser-based fluorescence microscopy." Microbiology Australia. 23 (1), pp. 16-17.
Article Title

Laser-based fluorescence microscopy

ERA Journal ID40629
Article CategoryArticle
Authors
AuthorLearmonth, Robert P.
Journal TitleMicrobiology Australia
Journal Citation23 (1), pp. 16-17
Number of Pages2
Year2002
PublisherCSIRO Publishing
Place of PublicationMelbourne, Australia
ISSN1324-4272
Web Address (URL)http://www.theasm.com.au/publications
Abstract

[Introduction]:Many microbiologists will be familiar with transmission fluorescence microscopy as well the improved epifluorescence microscopy. However, microbiologists
are generally less familiar with confocal laser scanning microscopy (CLSM), despite its use since the early 1980s in cell biology and related fields. Furthermore, the new technique of multi-photon laser scanning microscopy (MPLSM) is not yet widely appreciated. This article briefly outlines some principles and applications of these two relatively recent microscopic techniques. With their inherent advantages, CLSM and MPLSM could be widely applied to enhance microbiological studies.

Keywordsfluorescence, laser, scanning, microscopy, confocal, multi-photon
ANZSRC Field of Research 2020510203. Nonlinear optics and spectroscopy
510202. Lasers and quantum electronics
310799. Microbiology not elsewhere classified
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Byline AffiliationsDepartment of Biological and Physical Sciences
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