Disease development and enhanced susceptibility of wounded pepper roots to Phytophthora capsici
Article
Article Title | Disease development and enhanced susceptibility of wounded pepper roots to Phytophthora capsici |
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ERA Journal ID | 2652 |
Article Category | Article |
Authors | Adorada D. L., Biles, C. L., Liddell, C. M., Fernández-Pavía, S., Waugh, K. O. and Waugh, M. E. |
Journal Title | Plant Pathology |
Journal Citation | 49 (6), pp. 719-726 |
Year | 2000 |
Publisher | John Wiley & Sons |
Place of Publication | United Kingdom |
ISSN | 0032-0862 |
1365-3059 | |
Digital Object Identifier (DOI) | https://doi.org/10.1046/j.1365-3059.2000.00510.x |
Web Address (URL) | https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1046/j.1365-3059.2000.00510.x |
Abstract | Greenhouse experiments were conducted to determine disease progression of phytophthora root rot on nonwounded and wounded pepper plants (Capsicum annuum) and to determine whether susceptibility to Phytophthora capsici decreases with wound aging. Two isolates of P. capsici were used in this study, one less aggressive than the other. Trimming the roots immediately prior to inoculation with either isolate increased susceptibility significantly (P ≤ 0.05) compared with plant roots that were not trimmed. Both isolates caused a higher level of disease severity on disturbed/trimmed than on disturbed/nontrimmed roots. Disease also occurred earlier with the more aggressive isolate on both wounded and nonwounded roots. Disease severity was three to four times more severe on plants treated with the aggressive isolate (NM6011) than on those inoculated with the less aggressive isolate (NM6040), regardless of root treatment. In separate experiments, pepper roots were wounded and allowed to age for up to 5 days before inoculation. Resistance to P. capsici increased as the wounds aged, resulting in significantly (P ≤ 0.001) lower disease severity on plants with 3- and 5-day-old wounds than on those inoculated at the time of wounding and the nondisturbed/nontrimmed controls. Wounding of the roots followed by immediate inoculation with zoospores resulted in significantly higher levels of attachment than when roots were inoculated with zoospores 48 h after wounding. The 48-h postwounding inoculation treatment showed the same amount of zoospore attachment as nonwounded roots. Increase in plant resistance correlated (P ≤ 0.01) with an increase in total peroxidase activity. Isoelectric focusing-polyacrylamide gel electrophoresis (IEF-PAGE) indicated increased band intensity of three acidic and one basic isozyme as wounds aged. These data suggest that wound repair plays a role in decreasing infection and resultant disease symptoms of pepper to P. capsici. |
Keywords | Capsicum annuum; Infection studies; Peroxidase; Zoospores |
ANZSRC Field of Research 2020 | 300208. Farm management, rural management and agribusiness |
300409. Crop and pasture protection (incl. pests, diseases and weeds) | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | International Rice Research Institute, Philippines |
East Central University, United States | |
Paradigm Genetics, United States | |
New Mexico State University, United States | |
Genzyme Genetics, United States | |
National Center for Genome Research, United States |
https://research.usq.edu.au/item/qzy74/disease-development-and-enhanced-susceptibility-of-wounded-pepper-roots-to-phytophthora-capsici
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