Phylogeny, Ribosomal RNA Gene Typing and Relative Abundance of New Pseudomonas species (sensu stricto) isolated from Two Pinyon-Juniper Woodland Soils of the Arid Southwest U.S.
Article
Article Title | Phylogeny, Ribosomal RNA Gene Typing and Relative Abundance of New Pseudomonas species (sensu stricto) isolated from Two Pinyon-Juniper Woodland Soils of the Arid Southwest U.S. |
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ERA Journal ID | 2511 |
Article Category | Article |
Authors | Kuske, Cheryl R., Busch, Joseph D., Adorada, Dante L., Dunbar, John M. and Barns, Susan M. |
Journal Title | Systematic and Applied Microbiology |
Journal Citation | 22 (2), pp. 300-311 |
Number of Pages | 12 |
Year | 1999 |
Publisher | Elsevier |
ISSN | 0172-5564 |
0723-2020 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/S0723-2020(99)80077-0 |
Abstract | Rhizosphere-inhabiting Pseudomonas species interact with plant roots and may be important for plant performance under stressful environmental conditions. A comparison was conducted of culturable Pseudomonas isolates associated with pinyon rhizosphere and between-tree interspace areas in a hot, dry, volcanic cinder field and an adjacent sandy loam soil, in order to identify Pseudomonas species which may be involved in pinyon pine survival under stressful conditions. From a collection of 800 isolates, eleven isolates exhibiting different colony morphology were selected for 16S ribosomal RNA gene sequencing. Phylogenetic analysis of rDNA sequences from the eleven field isolates, forty-six described Pseudomonas species, and thirty-four previously characterized environmental isolates indicated that the isolates from the cinders and sandy loam soil clustered into three groups. The field isolates were distinct from any of the named species or other environmental isolates. Oligonucleotide primer pairs that differentiated three field isolate groups were designed from the 16S rDNA sequences, and eight hundred Pseudonomas field isolates cultured from pinyon rhizospheres and interspaces in the cinders and sandy loom soils were typed into the three groups using PCR assays. The composition of Pseudomonas populations in four environments was significantly different. The relative abundance of the three rDNA-based groups appeared to be affected by both the soil type and the pinyon rhizosphere. |
Keywords | 16A ribosomal RNA gene; PCR typing; Pseudomonas phylogeny; Rhizosphere bacteria; Soil bacteria |
ANZSRC Field of Research 2020 | 3001. Agricultural biotechnology |
3107. Microbiology | |
3099. Other agricultural, veterinary and food sciences | |
Public Notes | There are no files associated with this item. |
PubMed ID | 10390879 |
Byline Affiliations | Los Alamos National Laboratory, United States |
https://research.usq.edu.au/item/qzy75/phylogeny-ribosomal-rna-gene-typing-and-relative-abundance-of-new-pseudomonas-species-sensu-stricto-isolated-from-two-pinyon-juniper-woodland-soils-of-the-arid-southwest-u-s
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