Phylogeny, Ribosomal RNA Gene Typing and Relative Abundance of New Pseudomonas species (sensu stricto) isolated from Two Pinyon-Juniper Woodland Soils of the Arid Southwest U.S.

Article


Kuske, Cheryl R., Busch, Joseph D., Adorada, Dante L., Dunbar, John M. and Barns, Susan M.. 1999. "Phylogeny, Ribosomal RNA Gene Typing and Relative Abundance of New Pseudomonas species (sensu stricto) isolated from Two Pinyon-Juniper Woodland Soils of the Arid Southwest U.S. " Systematic and Applied Microbiology. 22 (2), pp. 300-311. https://doi.org/10.1016/S0723-2020(99)80077-0
Article Title

Phylogeny, Ribosomal RNA Gene Typing and Relative Abundance of New Pseudomonas species (sensu stricto) isolated from Two Pinyon-Juniper Woodland Soils of the Arid Southwest U.S.

ERA Journal ID2511
Article CategoryArticle
AuthorsKuske, Cheryl R., Busch, Joseph D., Adorada, Dante L., Dunbar, John M. and Barns, Susan M.
Journal TitleSystematic and Applied Microbiology
Journal Citation22 (2), pp. 300-311
Number of Pages12
Year1999
PublisherElsevier
ISSN0172-5564
0723-2020
Digital Object Identifier (DOI)https://doi.org/10.1016/S0723-2020(99)80077-0
Abstract

Rhizosphere-inhabiting Pseudomonas species interact with plant roots and may be important for plant performance under stressful environmental conditions. A comparison was conducted of culturable Pseudomonas isolates associated with pinyon rhizosphere and between-tree interspace areas in a hot, dry, volcanic cinder field and an adjacent sandy loam soil, in order to identify Pseudomonas species which may be involved in pinyon pine survival under stressful conditions. From a collection of 800 isolates, eleven isolates exhibiting different colony morphology were selected for 16S ribosomal RNA gene sequencing. Phylogenetic analysis of rDNA sequences from the eleven field isolates, forty-six described Pseudomonas species, and thirty-four previously characterized environmental isolates indicated that the isolates from the cinders and sandy loam soil clustered into three groups. The field isolates were distinct from any of the named species or other environmental isolates. Oligonucleotide primer pairs that differentiated three field isolate groups were designed from the 16S rDNA sequences, and eight hundred Pseudonomas field isolates cultured from pinyon rhizospheres and interspaces in the cinders and sandy loom soils were typed into the three groups using PCR assays. The composition of Pseudomonas populations in four environments was significantly different. The relative abundance of the three rDNA-based groups appeared to be affected by both the soil type and the pinyon rhizosphere.

Keywords16A ribosomal RNA gene; PCR typing; Pseudomonas phylogeny; Rhizosphere bacteria; Soil bacteria
ANZSRC Field of Research 20203001. Agricultural biotechnology
3107. Microbiology
3099. Other agricultural, veterinary and food sciences
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PubMed ID10390879
Byline AffiliationsLos Alamos National Laboratory, United States
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