The First Annotated Genome Assembly of Macrophomina tecta Associated with Charcoal Rot of Sorghum
Article
Article Title | The First Annotated Genome Assembly of Macrophomina tecta Associated with Charcoal Rot of Sorghum |
---|---|
ERA Journal ID | 122964 |
Article Category | Article |
Authors | Poudel, Barsha (Author), Purushotham, Neeraj (Author), Jones, Ashley (Author), Nasim, Jamila (Author), Adorada, Dante L. (Author), Sparks, Adam H. (Author), Schwessinger, Benjamin (Author) and Vaghefi, Niloofar (Author) |
Journal Title | Genome Biology and Evolution |
Journal Citation | 14 (6), pp. 1-7 |
Number of Pages | 7 |
Year | 2022 |
Publisher | Oxford University Press |
Place of Publication | United Kingdom |
ISSN | 1759-6653 |
Digital Object Identifier (DOI) | https://doi.org/10.1093/gbe/evac081 |
Web Address (URL) | https://academic.oup.com/gbe/article/14/6/evac081/6596626 |
Abstract | Charcoal rot is an important soilborne disease caused by a range of Macrophomina species, which affects a broad range of commercially important crops worldwide. Even though Macrophomina species are fungal pathogens of substantial economic importance, their mechanism of pathogenicity and host spectrum are poorly understood. There is an urgent need to better understand the biology, epidemiology, and evolution of Macrophomina species, which, in turn, will aid in improving charcoal rot management strategies. Here, we present the first high-quality genome assembly and annotation of Macrophomina tecta strain BRIP 70781 associated with charcoal rot symptoms on sorghum. Hybrid assembly integrating long reads generated by Oxford Nanopore Technology and short Illumina paired-end reads resulted in 43 contigs with a total assembly size of ∼54 Mb, and an N50 of 3.4 Mb. In total, 12,926 protein-coding genes and 7,036 repeats were predicted. Genome comparisons detected accumulation of DNA transposons in Macrophomina species associated with sorghum. The first reference genome of M. tecta generated in this study will contribute to more comparative and population genomics studies of Macrophomina species. |
Keywords | stalk/dry root rot, de novo assembly, genome annotation, transposable elements, Macrophomina phaseolina |
ANZSRC Field of Research 2020 | 310705. Mycology |
300409. Crop and pasture protection (incl. pests, diseases and weeds) | |
300209. Germplasm management | |
Byline Affiliations | Centre for Crop Health |
Australian National University | |
Institution of Origin | University of Southern Queensland |
Funding source | Grant ID GRDC 9176684 |
https://research.usq.edu.au/item/q77q9/the-first-annotated-genome-assembly-of-macrophomina-tecta-associated-with-charcoal-rot-of-sorghum
Download files
86
total views62
total downloads3
views this month0
downloads this month