Isolation of strains and their genome sequencing to analyze the mating system of Ophiocordyceps robertsii
Article
Xu, Melvin, Ashley, Nathan A., Vaghefi, Niloofar, Wilkinson, Ian and Idnurm, Alexander. 2023. "Isolation of strains and their genome sequencing to analyze the mating system of Ophiocordyceps robertsii." PLoS One. 18 (5). https://doi.org/10.1371/journal.pone.0284978
Article Title | Isolation of strains and their genome sequencing to analyze the mating system of Ophiocordyceps robertsii |
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ERA Journal ID | 39745 |
Article Category | Article |
Authors | Xu, Melvin, Ashley, Nathan A., Vaghefi, Niloofar, Wilkinson, Ian and Idnurm, Alexander |
Journal Title | PLoS One |
Journal Citation | 18 (5) |
Article Number | e0284978 |
Number of Pages | 14 |
Year | 2023 |
Publisher | Public Library of Science (PLoS) |
Place of Publication | United States |
ISSN | 1932-6203 |
Digital Object Identifier (DOI) | https://doi.org/10.1371/journal.pone.0284978 |
Web Address (URL) | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0284978 |
Abstract | The fungal genus Ophiocordyceps contains a number of insect pathogens. One of the best known of these is Ophiocordyceps sinensis, which is used in Chinese medicine and its overharvesting threatens sustainability; hence, alternative species are being sought. Ophiocordyceps robertsii, found in Australia and New Zealand, has been proposed to be a close relative to O. sinensis, but little is known about this species despite being also of historical significance. Here, O. robertsii strains were isolated into culture and high coverage draft genome sequences obtained and analyzed. This species has a large genome expansion, as also occurred in O. sinensis. The mating type locus was characterized, indicating a heterothallic arrangement whereby each strain has an idiomorphic region of two (MAT1-2-1, MAT1-2-2) or three (MAT1-1-1, MAT1-1-2, MAT1-1-3) genes flanked by the conserved APN2 and SLA2 genes. These resources provide a new opportunity for understanding the evolution of the expanded genome in the homothallic species O. sinensis, as well as capabilities to explore the pharmaceutical potential in a species endemic to Australia and New Zealand. |
Keywords | genome sequencing; mating system; Ophiocordyceps robertsii |
ANZSRC Field of Research 2020 | 300409. Crop and pasture protection (incl. pests, diseases and weeds) |
Byline Affiliations | University of Melbourne |
GhostMothLabs, Australia | |
Centre for Crop Health |
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https://research.usq.edu.au/item/z2728/isolation-of-strains-and-their-genome-sequencing-to-analyze-the-mating-system-of-ophiocordyceps-robertsii
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