Identification of the MAT1 locus in Stagonosporopsis tanaceti, and exploring its potential for sexual reproduction in Australian pyrethrum fields
Article
Article Title | Identification of the MAT1 locus in Stagonosporopsis tanaceti, and exploring its potential for sexual reproduction in Australian pyrethrum fields |
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ERA Journal ID | 2504 |
Article Category | Article |
Authors | Vaghefi, Niloofar (Author), Ades, Peter K. (Author), Hay, Frank S. (Author), Pethybridge, Sarah J. (Author), Ford, Rebecca (Author) and Taylor, Paul W. J. (Author) |
Journal Title | Fungal Biology |
Journal Citation | 119 (5), pp. 408-419 |
Number of Pages | 12 |
Year | 2015 |
Place of Publication | United Kingdom |
ISSN | 0953-7562 |
1469-8102 | |
1878-6146 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.funbio.2014.04.004 |
Web Address (URL) | http://ac.els-cdn.com/S1878614614000610/1-s2.0-S1878614614000610-main.pdf?_tid=20d2052c-87c0-11e7-af64-00000aab0f6b&acdnat=1503464672_c52d00ff91c22703f21ea3f72913b602 |
Abstract | Stagonosporopsis chrysanthemi, S. inoxydabilis, and S. tanaceti are closely related Ascomycetes associated with ray blight of the Asteraceae. To date, only S. tanaceti has been identified in Australia, incurring substantial losses to the pyrethrum industry. In contrast to the homothallic S. chrysanthemi and S. inoxydabilis, a sexual state has not been observed for S. tanaceti. The MAT1 locus in S. tanaceti was identified through de novo assembly of shotgun reads, and was further used to develop primers for amplification of the full-length MAT1/2 locus in S. chrysanthemi and S. inoxydabilis. As expected, S. chrysanthemi and S. inoxydabilis possessed a MAT1/2 locus typical of homothallic Dothideomycetes with two adjacent MAT1-1 and MAT1-2 idiomorphs. However, only MAT1-1 could be detected in the assembled genome of S. tanaceti. Although a sexual mode of reproduction cannot be ruled out for S. tanaceti, evidence so far suggests this is absent or occurring at very low frequency in Australian pyrethrum |
Keywords | chrysanthemi; Didymellaceae; inoxydabilis; mating type; ray blight |
ANZSRC Field of Research 2020 | 310805. Plant pathology |
310599. Genetics not elsewhere classified | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | University of Melbourne |
University of Tasmania | |
New Zealand Institute for Plant and Food Research Limited, New Zealand | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q460z/identification-of-the-mat1-locus-in-stagonosporopsis-tanaceti-and-exploring-its-potential-for-sexual-reproduction-in-australian-pyrethrum-fields
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