Development of microsatellite markers for quantifying genetic diversity of Pleospora betae
Presentation
Paper/Presentation Title | Development of microsatellite markers for quantifying genetic diversity of Pleospora betae |
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Presentation Type | Presentation |
Authors | Koenick, Lori (Author), Vaghefi, Niloofar (Author), Knight, Noel L. (Author), du Toit, Lindsey (Author) and Pethybridge, Sarah J. (Author) |
Journal or Proceedings Title | Phytopathology: International Journal of the American Phytopathological Society |
Journal Citation | 107 (12S), p. 201 |
Number of Pages | 1 |
Year | 2017 |
Publisher | American Phytopathological Society |
Place of Publication | St Paul, United States |
ISSN | 0031-949X |
1943-7684 | |
Web Address (URL) of Paper | https://apsjournals.apsnet.org/doi/10.1094/PHYTO-107-12-S5.196 |
Conference/Event | 2017 Annual Meeting of the Northeastern Division of the American Phytopathological Society |
Event Details | 2017 Annual Meeting of the Northeastern Division of the American Phytopathological Society Event Date 01 to end of 03 Nov 2017 Event Location Quebec City, Canada |
Abstract | Pleospora betae (syn. Phoma betae) is an important fungal pathogen of table beet, causing foliar disease, damping-off, and root decay. New York is the second largest producer of table beets in the United States for the processing industry and fresh markets. Microsatellite markers were developed for population genetics studies to quantify genetic and genotypic diversity of P. betae, and investigate hypotheses concerning sources of inoculum and disease spread. A de novo genome assembly of P. betae was used to identify microsatellite loci with tri- to hexa-nucleotide repeat motifs in non-exonic regions. Forty potential markers selected for primer development were tested on eight P. betae isolates. Fifteen of the markers were labeled with fluorescent dyes and tested on 24 P. betae isolates for reproducibility and polymorphism. Fragment analysis identified 11 of the markers to be reproducible (error rate = 0) with only one peak per isolate. These markers were highly polymorphic with an allelic range of four to 18 and an average of 7.4 alleles per locus. The markers were used to develop two sets of multiplex assays containing five and six markers, respectively. The microsatellite markers will be used to genotype P. betae populations from New York and the Pacific Northwest, the primary table beet seed production region of the United States. |
ANZSRC Field of Research 2020 | 310805. Plant pathology |
310599. Genetics not elsewhere classified | |
Public Notes | Abstracts only published from Conference. |
Byline Affiliations | Cornell University, United States |
Centre for Crop Health | |
Washington State University, United States | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q497w/development-of-microsatellite-markers-for-quantifying-genetic-diversity-of-pleospora-betae
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