Symbiotic N2-Fixer Community Composition, but Not Diversity, Shifts in Nodules of a Single Host Legume Across a 2-Million-Year Dune Chronosequence

Article


Birnbaum, Christina, Bissett, Andrew, Teste, Francois P. and Laliberte, Etienne. 2018. "Symbiotic N2-Fixer Community Composition, but Not Diversity, Shifts in Nodules of a Single Host Legume Across a 2-Million-Year Dune Chronosequence ." Microbial Ecology: an international journal. 76 (4), pp. 1009-1020. https://doi.org/10.1007/s00248-018-1185-1
Article Title

Symbiotic N2-Fixer Community Composition, but Not Diversity, Shifts in Nodules of a Single Host Legume Across a 2-Million-Year Dune Chronosequence

ERA Journal ID2493
Article CategoryArticle
AuthorsBirnbaum, Christina, Bissett, Andrew, Teste, Francois P. and Laliberte, Etienne
Journal TitleMicrobial Ecology: an international journal
Journal Citation76 (4), pp. 1009-1020
Number of Pages12
YearNov 2018
PublisherSpringer
Place of PublicationUnited States
ISSN0095-3628
1432-184X
Digital Object Identifier (DOI)https://doi.org/10.1007/s00248-018-1185-1
Web Address (URL)https://link.springer.com/article/10.1007/s00248-018-1185-1
Abstract

Long-term soil age gradients are useful model systems to study how changes in nutrient limitation shape communities of plant root mutualists because they represent strong natural gradients of nutrient availability, particularly of nitrogen (N) and phosphorus (P). Here, we investigated changes in the dinitrogen (N2)-fixing bacterial community composition and diversity in nodules of a single host legume (Acacia rostellifera) across the Jurien Bay chronosequence, a retrogressive 2 million-year-old sequence of coastal dunes representing an exceptionally strong natural soil fertility gradient. We collected nodules from plants grown in soils from five chronosequence stages ranging from very young (10s of years; associated with strong N limitation for plant growth) to very old (> 2,000,000 years; associated with strong P limitation), and sequenced the nifH gene in root nodules to determine the composition and diversity of N2-fixing bacterial symbionts. A total of 335 unique nifH gene operational taxonomic units (OTUs) were identified. Community composition of N2-fixing bacteria within nodules, but not diversity, changed with increasing soil age. These changes were attributed to pedogenesis-driven shifts in edaphic conditions, specifically pH, exchangeable manganese, resin-extractable phosphate, nitrate and nitrification rate. A large number of common N2-fixing bacteria genera (e.g. Bradyrhizobium, Ensifer, Mesorhizobium and Rhizobium) belonging to the Rhizobiaceae family (α-proteobacteria) comprised 70% of all raw sequences and were present in all nodules. However, the oldest soils, which show some of the lowest soil P availability ever recorded, harboured the largest proportion of unclassified OTUs, suggesting a unique set of N2-fixing bacteria adapted to extreme P limitation. Our results show that N2-fixing bacterial composition varies strongly during long-term ecosystem development, even within the same host, and therefore rhizobia show strong edaphic preferences.

KeywordsAcacia rostellifera; Rhizobia; Ecosystem development; Illumina sequencing; nifH
ANZSRC Field of Research 2020310308. Terrestrial ecology
310703. Microbial ecology
Public Notes

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Byline AffiliationsMurdoch University
Tulane University, United States
Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia
National University of San Luis, Argentina
University of Western Australia
University of Montreal, Canada
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https://research.usq.edu.au/item/v1z82/symbiotic-n2-fixer-community-composition-but-not-diversity-shifts-in-nodules-of-a-single-host-legume-across-a-2-million-year-dune-chronosequence

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