Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant-Pathogen Interactions
Article
Balotf, Sadegh, Wilson, Richard, Tegg, Robert S., Nichols, David S. and Wilson, Calum R.. 2022. "Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant-Pathogen Interactions." Proteomes. 10 (1). https://doi.org/10.3390/proteomes10010005
| Article Title | Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant-Pathogen Interactions |
|---|---|
| ERA Journal ID | 211256 |
| Article Category | Article |
| Authors | Balotf, Sadegh, Wilson, Richard, Tegg, Robert S., Nichols, David S. and Wilson, Calum R. |
| Journal Title | Proteomes |
| Journal Citation | 10 (1) |
| Article Number | 5 |
| Number of Pages | 14 |
| Year | Mar 2022 |
| Publisher | MDPI AG |
| Place of Publication | Switzerland |
| ISSN | 2227-7382 |
| Digital Object Identifier (DOI) | https://doi.org/10.3390/proteomes10010005 |
| Web Address (URL) | https://www.mdpi.com/2227-7382/10/1/5 |
| Abstract | The interaction between plants and pathogenic microorganisms is a multifaceted process mediated by both plant- and pathogen-derived molecules, including proteins, metabolites, and lipids. Large-scale proteome analysis can quantify the dynamics of proteins, biological pathways, and posttranslational modifications (PTMs) involved in the plant–pathogen interaction. Mass spectrometry (MS)-based proteomics has become the preferred method for characterizing proteins at the proteome and sub-proteome (e.g., the phosphoproteome) levels. MS-based proteomics can reveal changes in the quantitative state of a proteome and provide a foundation for understanding the mechanisms involved in plant–pathogen interactions. This review is intended as a primer for biologists that may be unfamiliar with the diverse range of methodology for MS-based shotgun proteomics, with a focus on techniques that have been used to investigate plant–pathogen interactions. We provide a summary of the essential steps required for shotgun proteomic studies of plants, pathogens and plant–pathogen interactions, including methods for protein digestion, identification, separation, and quantification. Finally, we discuss how protein PTMs may directly participate in the interaction between a pathogen and its host plant. |
| Keywords | mass spectrometry; shotgun proteomics; plant-pathogen interaction; post-translational modification |
| Article Publishing Charge (APC) Funding | Project Funding |
| Contains Sensitive Content | Does not contain sensitive content |
| ANZSRC Field of Research 2020 | 3001. Agricultural biotechnology |
| Byline Affiliations | University of Tasmania |
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