Differential expression of nitrate reductase in response to potassium and sodium nitrate: realtime PCR analysis
Article
Article Title | Differential expression of nitrate reductase in response to potassium and sodium nitrate: realtime PCR analysis |
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ERA Journal ID | 44764 |
Article Category | Article |
Authors | Balotf, Sadegh, Niazi, Ali, Kavoosi, Gholamreza and Ramezani, Amin |
Journal Title | Australian Journal of Crop Science |
Number of Pages | 5 |
Year | 2012 |
Publisher | Southern Cross Journals |
Place of Publication | Australia |
ISSN | 1835-2693 |
1835-2707 | |
Web Address (URL) | https://search.informit.org/doi/epdf/10.3316/informit.053708465371142 |
Abstract | Cucurbita pepo L. plants were grown in plastic pots with 2 kg washed sand per pot at three levels of potassium and sodium nitrate supplies to investigate the effects of nitrate supply on nitrate reductase activity and nitrate reductase expression in the leaves of the plants. Results demonstrated that starvation leads to decrease nitrate reductase activity and nitrate reductase expression. Supplementation of sodium and potassium nitrate had stimulatory effects on nitrate reductase activity and nitrate reductase expression in a dose dependent manner. However, potassium nitrate supplementation increased nitrate reductase activity and nitrate reductase expression more than sodium nitrate supplementation. In conclusion, both sodium and potassium nitrate, as inducers, had significant effects on both nitrate reductase activity and nitrate reductase expression in low concentrations. At high nitrate supplies, nitrate reductase activity and nitrate reductase expressions were suppressed probably due to nitrogen metabolite feedback inhibition and toxicity. |
Keywords | nitrate salts supply; nitrate reductase activity; nitrate accumulation; nitrite production; feedback inhibition |
Contains Sensitive Content | Does not contain sensitive content |
ANZSRC Field of Research 2020 | 3001. Agricultural biotechnology |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Shiraz University, Iran |
https://research.usq.edu.au/item/z746v/differential-expression-of-nitrate-reductase-in-response-to-potassium-and-sodium-nitrate-realtime-pcr-analysis
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