Differential expression of nitrate reductase in response to potassium and sodium nitrate: realtime PCR analysis

Article


Balotf, Sadegh, Niazi, Ali, Kavoosi, Gholamreza and Ramezani, Amin. 2012. "Differential expression of nitrate reductase in response to potassium and sodium nitrate: realtime PCR analysis." Australian Journal of Crop Science.
Article Title

Differential expression of nitrate reductase in response to potassium and sodium nitrate: realtime PCR analysis

ERA Journal ID44764
Article CategoryArticle
AuthorsBalotf, Sadegh, Niazi, Ali, Kavoosi, Gholamreza and Ramezani, Amin
Journal TitleAustralian Journal of Crop Science
Number of Pages5
Year2012
PublisherSouthern Cross Journals
Place of PublicationAustralia
ISSN1835-2693
1835-2707
Web Address (URL)https://search.informit.org/doi/epdf/10.3316/informit.053708465371142
Abstract

Cucurbita pepo L. plants were grown in plastic pots with 2 kg washed sand per pot at three levels of potassium and sodium nitrate supplies to investigate the effects of nitrate supply on nitrate reductase activity and nitrate reductase expression in the leaves of the plants. Results demonstrated that starvation leads to decrease nitrate reductase activity and nitrate reductase expression. Supplementation of sodium and potassium nitrate had stimulatory effects on nitrate reductase activity and nitrate reductase expression in a dose dependent manner. However, potassium nitrate supplementation increased nitrate reductase activity and nitrate reductase expression more than sodium nitrate supplementation. In conclusion, both sodium and potassium nitrate, as inducers, had significant effects on both nitrate reductase activity and nitrate reductase expression in low concentrations. At high nitrate supplies, nitrate reductase activity and nitrate reductase expressions were suppressed probably due to nitrogen metabolite feedback inhibition and toxicity.

Keywordsnitrate salts supply; nitrate reductase activity; nitrate accumulation; nitrite production; feedback inhibition
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 20203001. Agricultural biotechnology
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Byline AffiliationsShiraz University, Iran
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