In Planta Transcriptome and Proteome Profiles of Spongospora subterranea in Resistant and Susceptible Host Environments Illuminates Regulatory Principles Underlying Host-Pathogen Interaction

Article

Balotf, Sadegh, Wilson, Richard, Tegg, Robert S., Nichols, David S. and Wilson, Calum R.. 2021. "In Planta Transcriptome and Proteome Profiles of Spongospora subterranea in Resistant and Susceptible Host Environments Illuminates Regulatory Principles Underlying Host-Pathogen Interaction." Biology. 10 (9). https://doi.org/10.3390/biology10090840
Article Title

In Planta Transcriptome and Proteome Profiles of Spongospora subterranea in Resistant and Susceptible Host Environments Illuminates Regulatory Principles Underlying Host-Pathogen Interaction

ERA Journal ID210193
Article CategoryArticle
AuthorsBalotf, Sadegh, Wilson, Richard, Tegg, Robert S., Nichols, David S. and Wilson, Calum R.
Journal TitleBiology
Journal Citation10 (9)
Article Number840
Number of Pages14
Year2021
PublisherMDPI AG
Place of PublicationSwitzerland
ISSN2079-7737
Digital Object Identifier (DOI)https://doi.org/10.3390/biology10090840
Web Address (URL)https://www.mdpi.com/2079-7737/10/9/840
Abstract

Spongospora subterranea is an obligate biotrophic pathogen, causing substantial economic loss to potato industries globally. Currently, there are no fully effective management strategies for the control of potato diseases caused by S. subterranea. To further our understanding of S. subterranea biology during infection, we characterized the transcriptome and proteome of the pathogen during the invasion of roots of a susceptible and a resistant potato cultivar. A total of 7650 transcripts from S. subterranea were identified in the transcriptome analysis in which 1377 transcripts were differentially expressed between two cultivars. In proteome analysis, we identified 117 proteins with 42 proteins significantly changed in comparisons between resistant and susceptible cultivars. The functional annotation of transcriptome data indicated that the gene ontology terms related to the transportation and actin processes were induced in the resistant cultivar. The downregulation of enzyme activity and nucleic acid metabolism in the resistant cultivar suggests a probable influence of these processes in the virulence of S. subterranea. The protein analysis results indicated that the majority of differentially expressed proteins were related to the metabolic processes and transporter activity. The present study provides a comprehensive molecular insight into the multiple layers of gene regulation that contribute to S. subterranea infection and development in planta and illuminates the role of host immunity in affecting pathogen responses.

KeywordsSpongospora subterranea; potato; in planta analysis; transcriptomics; proteomics
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 20203001. Agricultural biotechnology
Byline AffiliationsUniversity of Tasmania
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