Nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase expression and activity in response to different nitrogen sources in nitrogen-starved wheat seedlings

Article

Balotf, Sadegh, Kavoosi, Gholamreza and Kholdebarin, Bahman. 2016. "Nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase expression and activity in response to different nitrogen sources in nitrogen-starved wheat seedlings." Biotechnology and Applied Biochemistry. 63 (2), pp. 220-229. https://doi.org/10.1002/bab.1362
Article Title

Nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase expression and activity in response to different nitrogen sources in nitrogen-starved wheat seedlings

ERA Journal ID3344
Article CategoryArticle
AuthorsBalotf, Sadegh, Kavoosi, Gholamreza and Kholdebarin, Bahman
Journal TitleBiotechnology and Applied Biochemistry
Journal Citation63 (2), pp. 220-229
Number of Pages10
Year2016
PublisherJohn Wiley & Sons
Place of PublicationUnited States
ISSN0885-4513
1470-8744
Digital Object Identifier (DOI)https://doi.org/10.1002/bab.1362
Web Address (URL)https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.1362
Abstract

The objective of this study was to examine the expression and activity of nitrate reductase (NR, EC 1.7.1.1), nitrite reductase (NiR, EC 1.7.2.2), glutamine synthetase (GS, EC 6.3.1.2), and glutamate synthase (GOGAT, EC 1.4.7.1) in response to potassium nitrate, ammonium chloride, and ammonium nitrate in nitrogen-starved wheat seedlings. Plants were grown in standard nutrient solution for 17 days and then subjected to nitrogen starvation for 7 days. The starved plants were supplied with potassium nitrate ammonium nitrate and ammonium chloride (50 mM) for 4 days and the leaves were harvested. The relative expression of NR, NiR, GS, and GOGAT as well as the enzyme activities were investigated. Nitrogen starvation caused a significant decrease both in transcript levels and in NR, NiR, GS, and GOGAT activities. Potassium nitrate and ammonium nitrate treatments restored NR, NiR, GS, and GOGAT expressions and activities. Ammonium chloride increased only the expressions and activities of GS and GOGAT in a dose-dependent manner. The results of our study highlight the differential effects between the type and the amount of nitrogen salts on NR, NiR, GS, and GOGAT activities in wheat seedlings while potassium nitrate being more effective.

Keywordswheat seedlings; glutamate synthase; glutamine synthetase; nitrite reductase; nitrate reductase
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 20203001. Agricultural biotechnology
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Byline AffiliationsShiraz University, Iran
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https://research.usq.edu.au/item/z7468/nitrate-reductase-nitrite-reductase-glutamine-synthetase-and-glutamate-synthase-expression-and-activity-in-response-to-different-nitrogen-sources-in-nitrogen-starved-wheat-seedlings

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