Nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase expression and activity in response to different nitrogen sources in nitrogen-starved wheat seedlings
Article
Article Title | Nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthase expression and activity in response to different nitrogen sources in nitrogen-starved wheat seedlings |
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ERA Journal ID | 3344 |
Article Category | Article |
Authors | Balotf, Sadegh, Kavoosi, Gholamreza and Kholdebarin, Bahman |
Journal Title | Biotechnology and Applied Biochemistry |
Journal Citation | 63 (2), pp. 220-229 |
Number of Pages | 10 |
Year | 2016 |
Publisher | John Wiley & Sons |
Place of Publication | United States |
ISSN | 0885-4513 |
1470-8744 | |
Digital Object Identifier (DOI) | https://doi.org/10.1002/bab.1362 |
Web Address (URL) | https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bab.1362 |
Abstract | The objective of this study was to examine the expression and activity of nitrate reductase (NR, EC 1.7.1.1), nitrite reductase (NiR, EC 1.7.2.2), glutamine synthetase (GS, EC 6.3.1.2), and glutamate synthase (GOGAT, EC 1.4.7.1) in response to potassium nitrate, ammonium chloride, and ammonium nitrate in nitrogen-starved wheat seedlings. Plants were grown in standard nutrient solution for 17 days and then subjected to nitrogen starvation for 7 days. The starved plants were supplied with potassium nitrate ammonium nitrate and ammonium chloride (50 mM) for 4 days and the leaves were harvested. The relative expression of NR, NiR, GS, and GOGAT as well as the enzyme activities were investigated. Nitrogen starvation caused a significant decrease both in transcript levels and in NR, NiR, GS, and GOGAT activities. Potassium nitrate and ammonium nitrate treatments restored NR, NiR, GS, and GOGAT expressions and activities. Ammonium chloride increased only the expressions and activities of GS and GOGAT in a dose-dependent manner. The results of our study highlight the differential effects between the type and the amount of nitrogen salts on NR, NiR, GS, and GOGAT activities in wheat seedlings while potassium nitrate being more effective. |
Keywords | wheat seedlings; glutamate synthase; glutamine synthetase; nitrite reductase; nitrate reductase |
Contains Sensitive Content | Does not contain sensitive content |
ANZSRC Field of Research 2020 | 3001. Agricultural biotechnology |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Shiraz University, Iran |
https://research.usq.edu.au/item/z7468/nitrate-reductase-nitrite-reductase-glutamine-synthetase-and-glutamate-synthase-expression-and-activity-in-response-to-different-nitrogen-sources-in-nitrogen-starved-wheat-seedlings
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