Analysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply

Article


Kavoosi, Gholamreza, Balotf, Sadegh, Eshghi, Homeira and Hasani, Hasan. 2014. "Analysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply." Molecular Biology Research Communications. 3 (2), pp. 75-84. https://doi.org/10.22099/MBRC.2014.1960
Article Title

Analysis of nitrate reductase mRNA expression and nitrate reductase activity in response to nitrogen supply

Article CategoryArticle
AuthorsKavoosi, Gholamreza, Balotf, Sadegh, Eshghi, Homeira and Hasani, Hasan
Journal TitleMolecular Biology Research Communications
Journal Citation3 (2), pp. 75-84
Number of Pages10
Year2014
PublisherShiraz University
Place of PublicationIran
ISSN2345-2005
2322-181X
Digital Object Identifier (DOI)https://doi.org/10.22099/MBRC.2014.1960
Web Address (URL)https://mbrc.shirazu.ac.ir/article_1960.html
Abstract

Nitrate is one of the major sources of nitrogen for the growth of plants. It is taken up by plant roots and transported to the leaves where it is reduced to nitrite in the. The main objective of this research was to investigate stimulatory effects of sodium nitrate, potassium nitrate, ammonia and urea on the production/generation of the nitrate
reductase mRNA in Triticum aestivum plants. The plants were grown in standard nutrient solution for 21 days and then starved in a media without nitrate for seven days. Starved plants were stimulated with various concentrations of sodium nitrate, potassium nitrate, ammonia and urea, and the expression of nitrate reductase mRNA was analyzed by real-time PCR. Our results indicated that starvation caused significant decrease in the production of nitrate reductase mRNA in the plant leaf. Sodium and potassium nitrate
were capable of restoring the production of nitrate mRNA in a dose-dependent manner, since 50 mM of each produced the highest level of the mRNA. The stimulatory effect of
potassium nitrate was higher than sodium nitrate, while ammonia and urea did not show such activity. At low concentrations, sodium nitrate and potassium nitrate caused
significant increase in the nitrate/nitrite mRNA production, whereas high concentrations of these salts suppressed the expression of this gene considerably.

KeywordsTriticum aestivum; Nitrate reductase; Potassium nitrate; nitrate
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 20203001. Agricultural biotechnology
Public Notes

The accessible file is the accepted version of the paper. Please refer to the URL for the published version.

Byline AffiliationsShiraz University, Iran
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