Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator
Article
Article Title | Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator |
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ERA Journal ID | 2587 |
Article Category | Article |
Authors | Vagi, Pal (Author), Caffi, Tito (Author), Vaczy, Kalman Zoltan (Author), Nemeth, Mark Z. (Author) and Kiss, Levente (Author) |
Journal Title | European Journal of Plant Pathology |
Journal Citation | 144 (4), pp. 799-802 |
Number of Pages | 4 |
Year | 2016 |
Publisher | Springer |
Place of Publication | Netherlands |
ISSN | 0929-1873 |
1573-8469 | |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s10658-015-0797-2 |
Web Address (URL) | http://link.springer.com/article/10.1007/s10658-015-0797-2 |
Abstract | The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia. |
Keywords | grapevine powdery mildew, fluorescein diacetate, ethidium bromide, propidium iodide, epifluorescence microscopy |
ANZSRC Field of Research 2020 | 300409. Crop and pasture protection (incl. pests, diseases and weeds) |
Public Notes | File reproduced in accordance with the copyright policy of the publisher/author. |
Byline Affiliations | Eotvos Lorand University (ELTE), Hungary |
Catholic University of Sacred Heart, Italy | |
Budapest Metropolitan University of Applied Sciences, Hungary | |
Hungarian Academy of Sciences, Hungary | |
Institution of Origin | University of Southern Queensland |
Funding source | Grant ID EU TAMOP-4.2.2D-15/1/Konv-2015-003 |
Funding source | Grant ID OTKA NN 100415 |
https://research.usq.edu.au/item/q3xyv/refining-a-method-for-ascospore-viability-testing-in-overwintering-chasmothecia-of-erysiphe-necator
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