Characterization of polygenic resistance to powdery mildew in tomato at cytological, biochemical and gene expression level
Article
Article Title | Characterization of polygenic resistance to powdery mildew in tomato at cytological, biochemical and gene expression level |
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ERA Journal ID | 2622 |
Article Category | Article |
Authors | Li, Chengwei (Author), Faino, Luigi (Author), Dong, Lin (Author), Fan, Junmei (Author), Kiss, Levente (Author), de Giovanni, Claudio (Author), Lebeda, Ales (Author), Scott, John (Author), Matsuda, Yoshinori (Author), Toyoda, Hideyoshi (Author), Lindhout, Pim (Author), Visser, Richard G. F. (Author), Bonnema, Guusje (Author) and Bai, Yuling (Author) |
Journal Title | Molecular Plant Pathology |
Journal Citation | 13 (2), pp. 148-159 |
Number of Pages | 11 |
Year | 2012 |
Place of Publication | United Kingdom |
ISSN | 1364-3703 |
1464-6722 | |
Digital Object Identifier (DOI) | https://doi.org/10.1111/j.1364-3703.2011.00737.x |
Web Address (URL) | http://onlinelibrary.wiley.com/doi/10.1111/j.1364-3703.2011.00737.x/abstract |
Abstract | Extensive research in the area of plant innate immunity has increased considerably our understanding of the molecular mechanisms associated with resistance controlled by a dominant resistance gene. In contrast, little is known about the molecular basis underlying the resistance conferred by quantitative trait loci (QTLs). In this study, using the interaction of tomato (Solanum lycopersicum) with Oidium neolycopersici, we compared the cytological, biochemical and molecular mechanisms involved in both monogenic and polygenic resistances conferred by a dominant gene (Ol-1) and three QTLs (Ol-qtls), respectively. Our results showed that the three Ol-qtls jointly confer a very high level of broad-spectrum resistance and that the resistance is associated with both the hypersensitive response and papillae formation, with the hypersensitive response being prevalent. Both H2O2 and callose accumulation, which are coupled with Ol-1-mediated resistance, are also associated with the resistance conferred by Ol-qtls. Further, we analysed the pathogen-induced transcript profiles of near-isogenic lines carrying the three Ol-qtls and the Ol-1 gene. Transcript profiles obtained by cDNA-amplified fragment length polymorphism analysis showed that, on fungal challenge, about 70% of the transcript-derived fragments are up-regulated in both susceptible and resistant genotypes. Most of the sequenced transcript-derived fragments showed homology to genes with functions in defence responses, suggesting that defence-responsive genes responsible for basal defence are involved in both monogenic and polygenic resistances conferred by Ol-1 and Ol-qtls, respectively. Although about 18% of the identified transcript-derived fragments are specific for either monogenic or polygenic resistance, their expression patterns need to be further verified by quantitative reverse transcriptase-polymerase chain reaction. |
Keywords | Ascomycota; disease resistance; gene expression profiling; gene expression regulation; plant genes, Glucans; Hydrogen Peroxide; inbreeding; Lycopersicon esculentum; multifactorial inheritance; plant diseases; plant leaves; reproducibility of results; Reverse Transcriptase Polymerase Chain Reaction; Messenger RNA; species specificity; tomato; Solanum lycopersicum; Oidium neolycopersici; ( Ol-1; Ol-qtls; |
ANZSRC Field of Research 2020 | 310805. Plant pathology |
310705. Mycology | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Wageningen University, Netherlands |
Hungarian Academy of Sciences, Hungary | |
University of Bari Aldo Moro, Italy | |
Palacky University Olomouc, Czech Republic | |
University of Florida, United States | |
Kindai University, Japan | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q4230/characterization-of-polygenic-resistance-to-powdery-mildew-in-tomato-at-cytological-biochemical-and-gene-expression-level
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