Growth Hormone Increases BDNF and mTOR Expression in Specific Brain Regions after Photothrombotic Stroke in Mice
Article
Article Title | Growth Hormone Increases BDNF and mTOR Expression in Specific Brain Regions after Photothrombotic Stroke in Mice |
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ERA Journal ID | 211137 |
Article Category | Article |
Authors | Sanchez-Bezanilla, Sonia (Author), Beard, Daniel J. (Author), Hood, Rebecca J. (Author), Aberg, N. David (Author), Crock, Patricia (Author), Walker, Frederick R. (Author), Nilsson, Michael (Author), Isgaard, Jorgen (Author) and Ong, Lin Kooi (Author) |
Journal Title | Neural Plasticity |
Journal Citation | 2022, pp. 1-13 |
Article Number | 9983042 |
Number of Pages | 13 |
Year | 2022 |
Place of Publication | United Kingdom |
ISSN | 1687-5443 |
2090-5904 | |
Digital Object Identifier (DOI) | https://doi.org/10.1155/2022/9983042 |
Web Address (URL) | https://www.hindawi.com/journals/np/2022/9983042/ |
Abstract | Aims. We have shown that growth hormone (GH) treatment poststroke increases neuroplasticity in peri-infarct areas and the hippocampus, improving motor and cognitive outcomes. We aimed to explore the mechanisms of GH treatment by investigating how GH modulates pathways known to induce neuroplasticity, focusing on association between brain-derived neurotrophic factor (BDNF) and mammalian target of rapamycin (mTOR) in the peri-infarct area, hippocampus, and thalamus. Methods. Recombinant human growth hormone (r-hGH) or saline was delivered (0.25 μl/hr, 0.04 mg/day) to mice for 28 days, commencing 48 hours after photothrombotic stroke. Protein levels of pro-BDNF, total-mTOR, phosphorylated-mTOR, total-p70S6K, and phosporylated-p70S6K within the peri-infarct area, hippocampus, and thalamus were evaluated by western blotting at 30 days poststroke. Results. r-hGH treatment significantly increased pro-BDNF in peri-infarct area, hippocampus, and thalamus (p<0.01). r-hGH treatment significantly increased expression levels of total-mTOR in the peri-infarct area and thalamus (p<0.05). r-hGH treatment significantly increased expression of total-p70S6K in the hippocampus (p<0.05). Conclusion. r-hGH increases pro-BDNF within the peri-infarct area and regions that are known to experience secondary neurodegeneration after stroke. Upregulation of total-mTOR protein expression in the peri-infarct and thalamus suggests that this might be a pathway that is involved in the neurorestorative effects previously reported in these animals and warrants further investigation. These findings suggest region-specific mechanisms of action of GH treatment and provide further understanding for how GH treatment promotes neurorestorative effects after stroke. |
Keywords | Animals; Brain; Brain-Derived Neurotrophic Factor; Growth Hormone; Human Growth Hormone; Infarction; Mammals; Mice; Ribosomal Protein S6 Kinases, 70-kDa; Stroke; TOR Serine-Threonine Kinases |
ANZSRC Field of Research 2020 | 321402. Clinical pharmacology and therapeutics |
320902. Cellular nervous system | |
320999. Neurosciences not elsewhere classified | |
Byline Affiliations | University of Newcastle |
University of Gothenburg, Sweden | |
Hunter Medical Research Institute, Australia | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q7489/growth-hormone-increases-bdnf-and-mtor-expression-in-specific-brain-regions-after-photothrombotic-stroke-in-mice
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