Reconsidering the role of glial cells in chronic stress-induced dopaminergic neurons loss within the substantia nigra? Friend or foe?
Article
Article Title | Reconsidering the role of glial cells in chronic stress-induced dopaminergic neurons loss within the substantia nigra? Friend or foe? |
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ERA Journal ID | 15461 |
Article Category | Article |
Authors | Ong, Lin Kooi, Zhao, Zidan, Kluge, Murielle, TeBay, Clifford, Zalewska, Katarzyna, Dickson, Phillip W., Johnson, Sarah J., Nilsson, Michael and Walker, Frederick R. |
Journal Title | Brain, Behavior, and Immunity |
Journal Citation | 60, pp. 117-125 |
Number of Pages | 9 |
Year | Feb 2017 |
Place of Publication | United States |
ISSN | 0889-1591 |
1090-2139 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.bbi.2016.10.001 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0889159116304548 |
Abstract | Exposure to psychological stress is known to seriously disrupt the operation of the substantia nigra (SN) and may in fact initiate the loss of dopaminergic neurons within the SN. In this study, we aimed to investigate how chronic stress modified the SN in adult male mice. Using a paradigm of repeated restraint stress (an average of 20 h per week for 6 weeks), we examined changes within the SN using western blotting and immunohistochemistry. We demonstrated that chronic stress was associated with a clear loss of dopaminergic neurons within the SN. The loss of dopaminergic neurons was accompanied by higher levels of oxidative stress damage, indexed by levels of protein carbonylation and strong suppression of both microglial and astrocytic responses. In addition, we demonstrated for the first time, that chronic stress alone enhanced the aggregation of α-synuclein into the insoluble protein fraction. These results indicate that chronic stress triggered loss of dopaminergic neurons by increasing oxidative stress, suppressing glial neuroprotective functions and enhancing the aggregation of the neurotoxic protein, α-synuclein. Collectively, these results reinforce the negative effects of chronic stress on the viability of dopaminergic cells within the SN. |
Keywords | alpha-Synuclein; Astrocytes; Chronic stress; Microglia; Neurodegeneration; Oxidative stress; Substantia nigra |
Public Notes | There are no files associated with this item. |
Byline Affiliations | University of Newcastle |
Hunter Medical Research Institute, Australia | |
NHMRC Centre of Research Excellence Stroke Rehabilitation and Brain Recovery, Australia |
https://research.usq.edu.au/item/y82z7/reconsidering-the-role-of-glial-cells-in-chronic-stress-induced-dopaminergic-neurons-loss-within-the-substantia-nigra-friend-or-foe
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