Improving Infection Resistance in Tissue Engineered Scaffolds for Tensile Applications Using Vancomycin-Embedded Melt Electrowritten Scaffolds

Article


Mathew, Asha, Devlin, Brenna L., Singh, Dilpreet, Paxton, Naomi C. and Woodruff, Maria A.. 2023. "Improving Infection Resistance in Tissue Engineered Scaffolds for Tensile Applications Using Vancomycin-Embedded Melt Electrowritten Scaffolds." Macromolecular Materials and Engineering. 308 (10). https://doi.org/10.1002/mame.202300168
Article Title

Improving Infection Resistance in Tissue Engineered Scaffolds for Tensile Applications Using Vancomycin-Embedded Melt Electrowritten Scaffolds

ERA Journal ID1682
Article CategoryArticle
AuthorsMathew, Asha, Devlin, Brenna L., Singh, Dilpreet, Paxton, Naomi C. and Woodruff, Maria A.
Journal TitleMacromolecular Materials and Engineering
Journal Citation308 (10)
Article Number2300168
Number of Pages9
Year2023
PublisherJohn Wiley & Sons
Place of PublicationGermany
ISSN1438-7492
1439-2054
Digital Object Identifier (DOI)https://doi.org/10.1002/mame.202300168
Web Address (URL)https://onlinelibrary.wiley.com/doi/10.1002/mame.202300168
AbstractIt is important to consider mechanical, biological, and antibacterial properties of scaffolds when used for tissue engineering applications. This study presents a method to create complex “wavy” architecture polycaprolactone (PCL) scaffolds toward the development of tissue engineered ligament and tendon tissue substitutes, fabricated using melt electrowriting (MEW) and loaded with vancomycin (5, 10, and 25% w/w). Scaffolds are characterized for both mechanical and biological properties. Loading PCL scaffolds with vancomycin with modified solvent evaporation technique achieves a high loading efficiency of maximum 18% w/w and high encapsulation efficiency with over 89%. Vancomycin loaded PCL scaffolds with all three doses (5, 10, and 25% w/w) display antibacterial activity against Gram-positive Staphylococcus aureus (S. aureus) up to 14 days of release. Initial burst followed by a sustained release is observed on all three vancomycin loaded scaffolds for up to 28 days. Importantly, in addition to antibacterial properties, vancomycin-loaded PCL scaffolds also display improved mechanical properties compared to traditional crosshatch design MEW scaffolds and are noncytotoxic at all concentrations as demonstrated by live-dead staining, cell attachment and proliferation assays indicating its potential as an effective treatment option for tissue regeneration in rotator cuff injuries or other tissues undergoing tensile biomechanical loading.
Keywordsdrug encapsulation and release; melt electrowriting; PCL; vancomycin
ANZSRC Field of Research 2020400301. Biofabrication
Byline AffiliationsQueensland University of Technology
University of Oregon, United States
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