Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector

Article

Mathew, Asha, Cao, Hongliang, Collin, Estelle, Wang, Wenxin and Pandit, Abhay. 2012. "Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector." International Journal of Pharmaceutics. 434 (1-2), pp. 99-105. https://doi.org/10.1016/j.ijpharm.2012.05.010
Article Title

Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector

ERA Journal ID14778
Article CategoryArticle
AuthorsMathew, Asha, Cao, Hongliang, Collin, Estelle, Wang, Wenxin and Pandit, Abhay
Journal TitleInternational Journal of Pharmaceutics
Journal Citation434 (1-2), pp. 99-105
Number of Pages7
Year2012
PublisherElsevier
Place of PublicationNetherlands
ISSN0378-5173
1873-3476
Digital Object Identifier (DOI)https://doi.org/10.1016/j.ijpharm.2012.05.010
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S037851731200467X
AbstractA unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine)dendrimer (dPAMAM; commercial name: SuperFect®) in fibroblasts and adipose tissue derived stem cells (ADSCs).
Keywords2-(Dimethylamino) ethyl methacrylate; Deactivation enhanced atom transfer radical polymerisation; Gene therapy
ANZSRC Field of Research 2020400302. Biomaterials
Public NotesFiles associated with this item cannot be displayed due to copyright restrictions.
Byline AffiliationsNational University of Ireland, Ireland
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