Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector

Article

Mathew, Asha, Cao, Hongliang, Collin, Estelle, Wang, Wenxin and Pandit, Abhay. 2012. "Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector." International Journal of Pharmaceutics. 434 (1-2), pp. 99-105. https://doi.org/10.1016/j.ijpharm.2012.05.010

Article Title	Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector
ERA Journal ID	14778
Article Category	Article
Authors	Mathew, Asha, Cao, Hongliang, Collin, Estelle, Wang, Wenxin and Pandit, Abhay
Journal Title	International Journal of Pharmaceutics
Journal Citation	434 (1-2), pp. 99-105
Number of Pages	7
Year	2012
Publisher	Elsevier
Place of Publication	Netherlands
ISSN	0378-5173
	1873-3476
Digital Object Identifier (DOI)	https://doi.org/10.1016/j.ijpharm.2012.05.010
Web Address (URL)	https://www.sciencedirect.com/science/article/pii/S037851731200467X
Abstract	A unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine)dendrimer (dPAMAM; commercial name: SuperFect®) in fibroblasts and adipose tissue derived stem cells (ADSCs).
Keywords	2-(Dimethylamino) ethyl methacrylate; Deactivation enhanced atom transfer radical polymerisation; Gene therapy
ANZSRC Field of Research 2020	400302. Biomaterials
Public Notes	Files associated with this item cannot be displayed due to copyright restrictions.
Byline Affiliations	National University of Ireland, Ireland

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