An ex-vivo multiple sclerosis model of inflammatory demyelination using hyperbranched polymer

Article

Mathew, Asha, Pakan, Janelle M.P., Collin, Estelle C., Wang, Wenxin, McDermott, Kieran W., Fitzgerald, Una, Reynolds, Richard and Pandit, Abhay S.. 2013. "An ex-vivo multiple sclerosis model of inflammatory demyelination using hyperbranched polymer." Biomaterials. 34 (23), pp. 5872-5882. https://doi.org/10.1016/j.biomaterials.2013.04.010
Article Title

An ex-vivo multiple sclerosis model of inflammatory demyelination using hyperbranched polymer

ERA Journal ID5035
Article CategoryArticle
AuthorsMathew, Asha, Pakan, Janelle M.P., Collin, Estelle C., Wang, Wenxin, McDermott, Kieran W., Fitzgerald, Una, Reynolds, Richard and Pandit, Abhay S.
Journal TitleBiomaterials
Journal Citation34 (23), pp. 5872-5882
Number of Pages11
Year2013
PublisherElsevier
Place of PublicationNetherlands
ISSN0142-9612
1878-5905
Digital Object Identifier (DOI)https://doi.org/10.1016/j.biomaterials.2013.04.010
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0142961213004547
AbstractMultiple sclerosis (MS) is characterized by the presence of inflammatory demyelinating foci throughout the brain and spinal cord, accompanied by axonal and neuronal damage. Although inflammatory processes are thought to underlie the pathological changes, the individual mediators of this damage are unclear. In order to study the role of pro-inflammatory cytokines in demyelination in the central nervous system, we have utilized a hyperbranched poly(2-dimethyl-aminoethylmethacrylate) based non-viral gene transfection system to establish an inflammatory demyelinating model of MS in an ex-vivo environment. The synthesized non-viral gene transfection system was optimized for efficient transfection with minimal cytotoxicity. Organotypic brain slices were then successfully transfected with the TNF or IFNγ genes. TNF and IFNγ expression and release in cerebellar slices via non-viral gene delivery approach resulted in inflammation mediated myelin loss, thus making it a promising ex-vivo approach for studying the underlying mechanisms of demyelination in myelin-related diseases such as MS.
KeywordsDemyelination; Inflammation; Multiple sclerosis; Non-viral gene delivery; Organotypic brain slice
ANZSRC Field of Research 2020400302. Biomaterials
Public NotesFiles associated with this item cannot be displayed due to copyright restrictions.
Byline AffiliationsNational University of Ireland, Ireland
University College Cork, Ireland
Imperial College London, United Kingdom
Permalink -

https://research.usq.edu.au/item/z30zz/an-ex-vivo-multiple-sclerosis-model-of-inflammatory-demyelination-using-hyperbranched-polymer

Log in to edit
  • 40
    total views
  • 0
    total downloads
  • 3
    views this month
  • 0
    downloads this month

Export as

Related outputs

Nano-roughness Modification of 3D printed Poly (lactic Acid) Polymer via Alkaline Wet Etching Towards Biomedical Applications
Kumara, S. P. S. N Buddhika Sampath, Senevirathne, S. W. M. A. Ishantha, Mathew, Asha, Ebenezer, Preetha, Yarlagadda, Tejasri, Bray, Laura, Mirkhalaf, Mohammad and Yarlagadda, Prasad K. D. V.. 2024. "Nano-roughness Modification of 3D printed Poly (lactic Acid) Polymer via Alkaline Wet Etching Towards Biomedical Applications." Journal of Applied Science and Engineering. 28 (6), pp. 1331-1340. https://doi.org/org/10.6180/jase.202506_28(6).0015
Improving Infection Resistance in Tissue Engineered Scaffolds for Tensile Applications Using Vancomycin-Embedded Melt Electrowritten Scaffolds
Mathew, Asha, Devlin, Brenna L., Singh, Dilpreet, Paxton, Naomi C. and Woodruff, Maria A.. 2023. "Improving Infection Resistance in Tissue Engineered Scaffolds for Tensile Applications Using Vancomycin-Embedded Melt Electrowritten Scaffolds." Macromolecular Materials and Engineering. 308 (10). https://doi.org/10.1002/mame.202300168
Progress in Nanostructured Mechano-Bactericidal Polymeric Surfaces for Biomedical Applications
Kumara, S. P. S. N. Buddhika Sampath, Senevirathne, S. W. M. Amal Ishantha, Mathew, Asha, Bray, Laura, Mirkhalaf, Mohammad and Yarlagadda, Prasad K.D.V.. 2023. "Progress in Nanostructured Mechano-Bactericidal Polymeric Surfaces for Biomedical Applications." Nanomaterials. 13 (20). https://doi.org/10.3390/nano13202799
Preferential adhesion of bacterial cells onto top- and bottom-mounted nanostructured surfaces under flow conditions
Senevirathne, S. W. M. A. Ishantha, Mathew, Asha, Toh, Yi Chin and Yarlagadda, Prasad K. D. V.. 2023. "Preferential adhesion of bacterial cells onto top- and bottom-mounted nanostructured surfaces under flow conditions." Nanoscale Advances. 5 (23). https://doi.org/10.1039/d3na00581j
Nanospikes on Customized 3D-Printed Titanium Implant Surface Inhibits Bacterial Colonization
Mathew, Asha, Hasan, Jafar, Singamneni, Sarat and Yarlagadda, Prasad K. D. V.. 2023. "Nanospikes on Customized 3D-Printed Titanium Implant Surface Inhibits Bacterial Colonization." Advanced Engineering Materials. 25 (8). https://doi.org/10.1002/adem.202201306
The Significance of Coordinated Research Against SARS-CoV-2
Hasan, Jafar, Velic, Amar, Jaggessar, Alka, Mathew, Asha, Yarlagadda, Tejasri, Spann, Kirsten, Ramarishna, Seeram and Yarlagadda, Prasad K. D. V.. 2022. "The Significance of Coordinated Research Against SARS-CoV-2." Batako, Andre, Burduk, Anna, Karyono, Kanisius, Chen, Xun and Wyczólkowski, Ryszard (ed.) 15 th Global Congress on Manufacturing and Management (GCMM 2021). Liverpool, United Kingdom 20 - 23 Apr 2021 Switzerland. https://doi.org/10.1007/978-3-030-90532-3_53
Bactericidal Efficacy of Nanostructured Surfaces Increases under Flow Conditions
Senevirathne, S. W. M. A. Ishantha, Mathew, Asha, Toh, Yi-Chin and Yarlagadda, Prasad K. D. V.. 2022. "Bactericidal Efficacy of Nanostructured Surfaces Increases under Flow Conditions ." ACS Omega. 7 (45), p. 41711–41722. https://doi.org/10.1021/acsomega.2c05828
Bactericidal efficiency of micro- and nanostructured surfaces: a critical perspective
Senevirathne, S. W. M. A. I., Hasan, J., Mathew, A., Woodruff, M. and Yarlagadda, P. K. D. V.. 2021. "Bactericidal efficiency of micro- and nanostructured surfaces: a critical perspective ." RSC Advances: an international journal to further the chemical sciences. 11 (3), pp. 1883-1900. https://doi.org/10.1039/d0ra08878a
Trends in Bactericidal Nanostructured Surfaces: An Analytical Perspective
Senevirathne, S. W. M. A. Ishantha, Hasan, Jafar, Mathew, Asha, Jaggessar, Alka and Yarlagadda, Prasad K.D.V.. 2021. "Trends in Bactericidal Nanostructured Surfaces: An Analytical Perspective ." ACS Applied Bio Materials. 4 (10), pp. 7626-7642. https://doi.org/10.1021/acsabm.1c00839
Adaptations and lessons from COVID-19: A perspective on how some industries will be impacted
Velic, Amar, Jaggessar, Alka, Senevirathne, Amal, Mathew, Asha, Paritala, Paritala, Phani, Islam, Majedul, Panicker, Arpana Gopi, Prasad, Karthika, Yarlagadda, Teja, Spann, Kirsten, Hasan, Jafar and Yarlagadda, Prasad K. D. V.. 2020. "Adaptations and lessons from COVID-19: A perspective on how some industries will be impacted." Advanced Materials Letter. 11 (7). https://doi.org/10.5185/amlett.2020.071533
Multi-biofunctional properties of three species of cicada wings and biomimetic fabrication of nanopatterned titanium pillars
Shahali, Hesam, Hasan, Jafar, Mathew, Asha, Wang, Hongxia, Yan, Cheng, Tesfamichael, Tuquabo and Yarlagadda, Prasad K. D. V.. 2019. "Multi-biofunctional properties of three species of cicada wings and biomimetic fabrication of nanopatterned titanium pillars." Journal of Materials Chemistry B. 7 (8), pp. 1300-1310. https://doi.org/10.1039/c8tb03295e
Bacteria Death and Osteoblast Metabolic Activity Correlated to Hydrothermally Synthesised TiO2 Surface Properties
Jaggessar, Alka, Mathew, Asha, Tesfamichael, Tuquabo, Wang, Hongxia, Yan, Cheng and Yarlagadda, Prasad KDV. 2019. "Bacteria Death and Osteoblast Metabolic Activity Correlated to Hydrothermally Synthesised TiO2 Surface Properties ." Molecules. 24 (7). https://doi.org/10.3390/molecules24071201
Control of bacterial attachment by fracture topography
Velic, Amar, Mathew, Asha, Hines, Peter and Yarlagadda, Prasad K.D.V.. 2019. "Control of bacterial attachment by fracture topography ." Journal of The Mechanical Behavior of Biomedical Materials. 91, pp. 416-424. https://doi.org/10.1016/j.jmbbm.2018.10.020
Mechanical, bactericidal and osteogenic behaviours of hydrothermally synthesised TiO2 nanowire arrays
Jaggessar, Alka, Mathew, Asha, Wang, Hongxia, Tesfamichael, Tuquabo, Yan, Cheng and Yarlagadda, Prasad KDV. 2018. "Mechanical, bactericidal and osteogenic behaviours of hydrothermally synthesised TiO2 nanowire arrays ." Journal of The Mechanical Behavior of Biomedical Materials. 80, pp. 311-319. https://doi.org/10.1016/j.jmbbm.2018.02.011
Bio-mimicking nano and micro-structured surface fabrication for antibacterial properties in medical implants
Jaggessar, Alka, Shahali, Hesam, Mathew, Asha and Yarlagadda, Prasad K. D. V.. 2017. "Bio-mimicking nano and micro-structured surface fabrication for antibacterial properties in medical implants ." Journal of Nanobiotechnology. 15, pp. 1-20. https://doi.org/10.1186/s12951-017-0306-1
Antimicrobial and immunomodulatory surface-functionalized electrospun membranes for bone regeneration
Mathew, Asha, Vaquette, Cedryck, Hashimi, Saeed, Rathnayake, Irani, Huygens, Flavia, Hutmacher, Dietmar and Ivanovski, Saso. 2017. "Antimicrobial and immunomodulatory surface-functionalized electrospun membranes for bone regeneration." Advanced Healthcare Materials. 6 (10). https://doi.org/10.1002/adhm.201601345
Cationic polymers in the central nervous system: Past, present and future
Mathew, Asha, Morey, Mangesh and Pandit, Abhay. 2015. "Cationic polymers in the central nervous system: Past, present and future." Samal, Sangram K. and Dubruel, Peter (ed.) Cationic polymers in Regenerative medicine. United Kingdom. Royal Society of Chemistry (RSC). pp. 463-478
Periodontal tissue graft
Farag, Amro, Mathew, Asha, Vaquette, Cedryck, Hutmacher, Dietmar and Ivanovski, Saso. 2014. Periodontal tissue graft. AU2014903870
Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector
Mathew, Asha, Cao, Hongliang, Collin, Estelle, Wang, Wenxin and Pandit, Abhay. 2012. "Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector." International Journal of Pharmaceutics. 434 (1-2), pp. 99-105. https://doi.org/10.1016/j.ijpharm.2012.05.010
Transfection of macrophages by collagen hollow spheres loaded with polyplexes: A step towards modulating inflammation
Helary, Christophe, Browne, Shane, Mathew, Asha, Wang, Wenxin and Pandit, Pandit. 2012. "Transfection of macrophages by collagen hollow spheres loaded with polyplexes: A step towards modulating inflammation." Acta Biomaterialia. 8 (12), pp. 4208-4214. https://doi.org/10.1016/j.actbio.2012.06.017
Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot
Dash, Biraja C., Mahor, Sunil, Carroll, Oliver, Mathew, Asha, Wang, Wenxin, Woodhouse, Kimberly A. and Pandit, Abhay. 2011. "Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot." Journal of Controlled Release. 152 (3), pp. 382-392. https://doi.org/10.1016/j.jconrel.2011.03.006
Dual stimuli responsive PEG based hyperbranched polymers
Dong, Yixiao, Gunning, Paul, Cao, Hongliang, Mathew, Asha, Newland, Ben, Saeed, Aram Omer, Magnusson, Johannes Pall, Alexander, Cameron, Tai, Hongyun, Pandit, Abhay and Wang, Wenxin. 2010. "Dual stimuli responsive PEG based hyperbranched polymers." Polymer Chemistry. 1 (6), pp. 827-830. https://doi.org/10.1039/c0py00101e
Preparation of chitosan/polyglutamic acid spheres based on the use of polystyrene template as a nonviral gene carrier
Réthoré, Gildas, Mathew, Asha, Naik, Hemantkumar and Pandit, Abhay. 2009. "Preparation of chitosan/polyglutamic acid spheres based on the use of polystyrene template as a nonviral gene carrier." Tissue Engineering Part C: Methods. 15 (4), pp. 605-613. https://doi.org/10.1089/ten.tec.2008.0581