An ex-vivo multiple sclerosis model of inflammatory demyelination using hyperbranched polymer
Article
Mathew, Asha, Pakan, Janelle M.P., Collin, Estelle C., Wang, Wenxin, McDermott, Kieran W., Fitzgerald, Una, Reynolds, Richard and Pandit, Abhay S.. 2013. "An ex-vivo multiple sclerosis model of inflammatory demyelination using hyperbranched polymer." Biomaterials. 34 (23), pp. 5872-5882. https://doi.org/10.1016/j.biomaterials.2013.04.010
Article Title | An ex-vivo multiple sclerosis model of inflammatory demyelination using hyperbranched polymer |
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ERA Journal ID | 5035 |
Article Category | Article |
Authors | Mathew, Asha, Pakan, Janelle M.P., Collin, Estelle C., Wang, Wenxin, McDermott, Kieran W., Fitzgerald, Una, Reynolds, Richard and Pandit, Abhay S. |
Journal Title | Biomaterials |
Journal Citation | 34 (23), pp. 5872-5882 |
Number of Pages | 11 |
Year | 2013 |
Publisher | Elsevier |
Place of Publication | Netherlands |
ISSN | 0142-9612 |
1878-5905 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.biomaterials.2013.04.010 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0142961213004547 |
Abstract | Multiple sclerosis (MS) is characterized by the presence of inflammatory demyelinating foci throughout the brain and spinal cord, accompanied by axonal and neuronal damage. Although inflammatory processes are thought to underlie the pathological changes, the individual mediators of this damage are unclear. In order to study the role of pro-inflammatory cytokines in demyelination in the central nervous system, we have utilized a hyperbranched poly(2-dimethyl-aminoethylmethacrylate) based non-viral gene transfection system to establish an inflammatory demyelinating model of MS in an ex-vivo environment. The synthesized non-viral gene transfection system was optimized for efficient transfection with minimal cytotoxicity. Organotypic brain slices were then successfully transfected with the TNF or IFNγ genes. TNF and IFNγ expression and release in cerebellar slices via non-viral gene delivery approach resulted in inflammation mediated myelin loss, thus making it a promising ex-vivo approach for studying the underlying mechanisms of demyelination in myelin-related diseases such as MS. |
Keywords | Demyelination; Inflammation; Multiple sclerosis; Non-viral gene delivery; Organotypic brain slice |
ANZSRC Field of Research 2020 | 400302. Biomaterials |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | National University of Ireland, Ireland |
University College Cork, Ireland | |
Imperial College London, United Kingdom |
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