Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot

Article


Dash, Biraja C., Mahor, Sunil, Carroll, Oliver, Mathew, Asha, Wang, Wenxin, Woodhouse, Kimberly A. and Pandit, Abhay. 2011. "Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot." Journal of Controlled Release. 152 (3), pp. 382-392. https://doi.org/10.1016/j.jconrel.2011.03.006
Article Title

Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot

ERA Journal ID14794
Article CategoryArticle
AuthorsDash, Biraja C., Mahor, Sunil, Carroll, Oliver, Mathew, Asha, Wang, Wenxin, Woodhouse, Kimberly A. and Pandit, Abhay
Journal TitleJournal of Controlled Release
Journal Citation152 (3), pp. 382-392
Number of Pages11
Year2011
PublisherElsevier
Place of PublicationNetherlands
ISSN0168-3659
1873-4995
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jconrel.2011.03.006
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0168365911001428
AbstractSelf-assembly driven processes can be utilized to produce a variety of nanostructures useful for various in vitro and in vivo applications. Characteristics such as size, stability, biocompatibility, high therapeutic loading and controlled delivery of these nanostructures are particularly crucial in relation to in vivo applications. In this study, we report the fabrication of tunable monodispersed elastin-like polypeptide (ELP) hollow spheres of 100, 300, 500 and 1000 nm by exploiting the self-assembly property and net positive charge of ELP. The microbial transglutaminase (mTGase) cross-linking provided robustness and stability to the hollow spheres while maintaining surface functional groups for further modifications. The resulting hollow spheres showed a higher loading efficiency of plasmid DNA (pDNA) by using polyplex (~ 70 μg pDNA/mg of hollow sphere) than that of self-assembled ELP particles and demonstrated controlled release triggered by protease and elastase. Moreover, polyplex-loaded hollow spheres showed better cell viability than polyplex alone and yielded higher luciferase expression by providing protection against endosomal degradation. Overall, the monodispersed, tunable hollow spheres with a capability of post-functionalization can provide an exciting new opportunity for use in a range of therapeutic and diagnostic applications.
KeywordsElastin-like polypeptide; Hollow sphere and transfection; Monodispersed; Self-assembly; Tunable
ANZSRC Field of Research 2020400302. Biomaterials
400311. Tissue engineering
Public NotesFiles associated with this item cannot be displayed due to copyright restrictions.
Byline AffiliationsNational University of Ireland, Ireland
Queen's University, Canada
Permalink -

https://research.usq.edu.au/item/z3102/tunable-elastin-like-polypeptide-hollow-sphere-as-a-high-payload-and-controlled-delivery-gene-depot

  • 35
    total views
  • 1
    total downloads
  • 1
    views this month
  • 0
    downloads this month

Export as

Related outputs

Nano-roughness Modification of 3D printed Poly (lactic Acid) Polymer via Alkaline Wet Etching Towards Biomedical Applications
Kumara, S. P. S. N Buddhika Sampath, Senevirathne, S. W. M. A. Ishantha, Mathew, Asha, Ebenezer, Preetha, Yarlagadda, Tejasri, Bray, Laura, Mirkhalaf, Mohammad and Yarlagadda, Prasad K. D. V.. 2024. "Nano-roughness Modification of 3D printed Poly (lactic Acid) Polymer via Alkaline Wet Etching Towards Biomedical Applications." Journal of Applied Science and Engineering. 28 (6), pp. 1331-1340. https://doi.org/org/10.6180/jase.202506_28(6).0015
Improving Infection Resistance in Tissue Engineered Scaffolds for Tensile Applications Using Vancomycin-Embedded Melt Electrowritten Scaffolds
Mathew, Asha, Devlin, Brenna L., Singh, Dilpreet, Paxton, Naomi C. and Woodruff, Maria A.. 2023. "Improving Infection Resistance in Tissue Engineered Scaffolds for Tensile Applications Using Vancomycin-Embedded Melt Electrowritten Scaffolds." Macromolecular Materials and Engineering. 308 (10). https://doi.org/10.1002/mame.202300168
Progress in Nanostructured Mechano-Bactericidal Polymeric Surfaces for Biomedical Applications
Kumara, S. P. S. N. Buddhika Sampath, Senevirathne, S. W. M. Amal Ishantha, Mathew, Asha, Bray, Laura, Mirkhalaf, Mohammad and Yarlagadda, Prasad K.D.V.. 2023. "Progress in Nanostructured Mechano-Bactericidal Polymeric Surfaces for Biomedical Applications." Nanomaterials. 13 (20). https://doi.org/10.3390/nano13202799
Preferential adhesion of bacterial cells onto top- and bottom-mounted nanostructured surfaces under flow conditions
Senevirathne, S. W. M. A. Ishantha, Mathew, Asha, Toh, Yi Chin and Yarlagadda, Prasad K. D. V.. 2023. "Preferential adhesion of bacterial cells onto top- and bottom-mounted nanostructured surfaces under flow conditions." Nanoscale Advances. 5 (23). https://doi.org/10.1039/d3na00581j
Nanospikes on Customized 3D-Printed Titanium Implant Surface Inhibits Bacterial Colonization
Mathew, Asha, Hasan, Jafar, Singamneni, Sarat and Yarlagadda, Prasad K. D. V.. 2023. "Nanospikes on Customized 3D-Printed Titanium Implant Surface Inhibits Bacterial Colonization." Advanced Engineering Materials. 25 (8). https://doi.org/10.1002/adem.202201306
The Significance of Coordinated Research Against SARS-CoV-2
Hasan, Jafar, Velic, Amar, Jaggessar, Alka, Mathew, Asha, Yarlagadda, Tejasri, Spann, Kirsten, Ramarishna, Seeram and Yarlagadda, Prasad K. D. V.. 2022. "The Significance of Coordinated Research Against SARS-CoV-2." Batako, Andre, Burduk, Anna, Karyono, Kanisius, Chen, Xun and Wyczólkowski, Ryszard (ed.) 15 th Global Congress on Manufacturing and Management (GCMM 2021). Liverpool, United Kingdom 20 - 23 Apr 2021 Switzerland. https://doi.org/10.1007/978-3-030-90532-3_53
Bactericidal Efficacy of Nanostructured Surfaces Increases under Flow Conditions
Senevirathne, S. W. M. A. Ishantha, Mathew, Asha, Toh, Yi-Chin and Yarlagadda, Prasad K. D. V.. 2022. "Bactericidal Efficacy of Nanostructured Surfaces Increases under Flow Conditions ." ACS Omega. 7 (45), p. 41711–41722. https://doi.org/10.1021/acsomega.2c05828
Trends in Bactericidal Nanostructured Surfaces: An Analytical Perspective
Senevirathne, S. W. M. A. Ishantha, Hasan, Jafar, Mathew, Asha, Jaggessar, Alka and Yarlagadda, Prasad K.D.V.. 2021. "Trends in Bactericidal Nanostructured Surfaces: An Analytical Perspective ." ACS Applied Bio Materials. 4 (10), pp. 7626-7642. https://doi.org/10.1021/acsabm.1c00839
Bactericidal efficiency of micro- and nanostructured surfaces: a critical perspective
Senevirathne, S. W. M. A. I., Hasan, J., Mathew, A., Woodruff, M. and Yarlagadda, P. K. D. V.. 2021. "Bactericidal efficiency of micro- and nanostructured surfaces: a critical perspective ." RSC Advances: an international journal to further the chemical sciences. 11 (3), pp. 1883-1900. https://doi.org/10.1039/d0ra08878a
Adaptations and lessons from COVID-19: A perspective on how some industries will be impacted
Velic, Amar, Jaggessar, Alka, Senevirathne, Amal, Mathew, Asha, Paritala, Paritala, Phani, Islam, Majedul, Panicker, Arpana Gopi, Prasad, Karthika, Yarlagadda, Teja, Spann, Kirsten, Hasan, Jafar and Yarlagadda, Prasad K. D. V.. 2020. "Adaptations and lessons from COVID-19: A perspective on how some industries will be impacted." Advanced Materials Letter. 11 (7). https://doi.org/10.5185/amlett.2020.071533
Multi-biofunctional properties of three species of cicada wings and biomimetic fabrication of nanopatterned titanium pillars
Shahali, Hesam, Hasan, Jafar, Mathew, Asha, Wang, Hongxia, Yan, Cheng, Tesfamichael, Tuquabo, Yarlagadda, Prasad K. D. V. and Murray, P.. 2019. "Multi-biofunctional properties of three species of cicada wings and biomimetic fabrication of nanopatterned titanium pillars." Journal of Materials Chemistry B. 7 (8), pp. 1300-1310. https://doi.org/10.1039/c8tb03295e
Bacteria Death and Osteoblast Metabolic Activity Correlated to Hydrothermally Synthesised TiO2 Surface Properties
Jaggessar, Alka, Mathew, Asha, Tesfamichael, Tuquabo, Wang, Hongxia, Yan, Cheng and Yarlagadda, Prasad KDV. 2019. "Bacteria Death and Osteoblast Metabolic Activity Correlated to Hydrothermally Synthesised TiO2 Surface Properties ." Molecules. 24 (7). https://doi.org/10.3390/molecules24071201
Control of bacterial attachment by fracture topography
Velic, Amar, Mathew, Asha, Hines, Peter and Yarlagadda, Prasad K.D.V.. 2019. "Control of bacterial attachment by fracture topography ." Journal of The Mechanical Behavior of Biomedical Materials. 91, pp. 416-424. https://doi.org/10.1016/j.jmbbm.2018.10.020
Mechanical, bactericidal and osteogenic behaviours of hydrothermally synthesised TiO2 nanowire arrays
Jaggessar, Alka, Mathew, Asha, Wang, Hongxia, Tesfamichael, Tuquabo, Yan, Cheng and Yarlagadda, Prasad KDV. 2018. "Mechanical, bactericidal and osteogenic behaviours of hydrothermally synthesised TiO2 nanowire arrays ." Journal of The Mechanical Behavior of Biomedical Materials. 80, pp. 311-319. https://doi.org/10.1016/j.jmbbm.2018.02.011
Bio-mimicking nano and micro-structured surface fabrication for antibacterial properties in medical implants
Jaggessar, Alka, Shahali, Hesam, Mathew, Asha and Yarlagadda, Prasad K. D. V.. 2017. "Bio-mimicking nano and micro-structured surface fabrication for antibacterial properties in medical implants ." Journal of Nanobiotechnology. 15, pp. 1-20. https://doi.org/10.1186/s12951-017-0306-1
Antimicrobial and immunomodulatory surface-functionalized electrospun membranes for bone regeneration
Mathew, Asha, Vaquette, Cedryck, Hashimi, Saeed, Rathnayake, Irani, Huygens, Flavia, Hutmacher, Dietmar and Ivanovski, Saso. 2017. "Antimicrobial and immunomodulatory surface-functionalized electrospun membranes for bone regeneration." Advanced Healthcare Materials. 6 (10). https://doi.org/10.1002/adhm.201601345
Cationic polymers in the central nervous system: Past, present and future
Mathew, Asha, Morey, Mangesh and Pandit, Abhay. 2015. "Cationic polymers in the central nervous system: Past, present and future." Samal, Sangram K. and Dubruel, Peter (ed.) Cationic polymers in Regenerative medicine. United Kingdom. Royal Society of Chemistry (RSC). pp. 463-478
Periodontal tissue graft
Farag, Amro, Mathew, Asha, Vaquette, Cedryck, Hutmacher, Dietmar and Ivanovski, Saso. 2014. Periodontal tissue graft. AU2014903870
An ex-vivo multiple sclerosis model of inflammatory demyelination using hyperbranched polymer
Mathew, Asha, Pakan, Janelle M.P., Collin, Estelle C., Wang, Wenxin, McDermott, Kieran W., Fitzgerald, Una, Reynolds, Richard and Pandit, Abhay S.. 2013. "An ex-vivo multiple sclerosis model of inflammatory demyelination using hyperbranched polymer." Biomaterials. 34 (23), pp. 5872-5882. https://doi.org/10.1016/j.biomaterials.2013.04.010
Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector
Mathew, Asha, Cao, Hongliang, Collin, Estelle, Wang, Wenxin and Pandit, Abhay. 2012. "Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector." International Journal of Pharmaceutics. 434 (1-2), pp. 99-105. https://doi.org/10.1016/j.ijpharm.2012.05.010
Transfection of macrophages by collagen hollow spheres loaded with polyplexes: A step towards modulating inflammation
Helary, Christophe, Browne, Shane, Mathew, Asha, Wang, Wenxin and Pandit, Pandit. 2012. "Transfection of macrophages by collagen hollow spheres loaded with polyplexes: A step towards modulating inflammation." Acta Biomaterialia. 8 (12), pp. 4208-4214. https://doi.org/10.1016/j.actbio.2012.06.017
Dual stimuli responsive PEG based hyperbranched polymers
Dong, Yixiao, Gunning, Paul, Cao, Hongliang, Mathew, Asha, Newland, Ben, Saeed, Aram Omer, Magnusson, Johannes Pall, Alexander, Cameron, Tai, Hongyun, Pandit, Abhay and Wang, Wenxin. 2010. "Dual stimuli responsive PEG based hyperbranched polymers." Polymer Chemistry. 1 (6), pp. 827-830. https://doi.org/10.1039/c0py00101e
Preparation of chitosan/polyglutamic acid spheres based on the use of polystyrene template as a nonviral gene carrier
Réthoré, Gildas, Mathew, Asha, Naik, Hemantkumar and Pandit, Abhay. 2009. "Preparation of chitosan/polyglutamic acid spheres based on the use of polystyrene template as a nonviral gene carrier." Tissue Engineering Part C: Methods. 15 (4), pp. 605-613. https://doi.org/10.1089/ten.tec.2008.0581