Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot

Article

Dash, Biraja C., Mahor, Sunil, Carroll, Oliver, Mathew, Asha, Wang, Wenxin, Woodhouse, Kimberly A. and Pandit, Abhay. 2011. "Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot." Journal of Controlled Release. 152 (3), pp. 382-392. https://doi.org/10.1016/j.jconrel.2011.03.006
Article Title

Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot

ERA Journal ID14794
Article CategoryArticle
AuthorsDash, Biraja C., Mahor, Sunil, Carroll, Oliver, Mathew, Asha, Wang, Wenxin, Woodhouse, Kimberly A. and Pandit, Abhay
Journal TitleJournal of Controlled Release
Journal Citation152 (3), pp. 382-392
Number of Pages11
Year2011
PublisherElsevier
Place of PublicationNetherlands
ISSN0168-3659
1873-4995
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jconrel.2011.03.006
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0168365911001428
AbstractSelf-assembly driven processes can be utilized to produce a variety of nanostructures useful for various in vitro and in vivo applications. Characteristics such as size, stability, biocompatibility, high therapeutic loading and controlled delivery of these nanostructures are particularly crucial in relation to in vivo applications. In this study, we report the fabrication of tunable monodispersed elastin-like polypeptide (ELP) hollow spheres of 100, 300, 500 and 1000 nm by exploiting the self-assembly property and net positive charge of ELP. The microbial transglutaminase (mTGase) cross-linking provided robustness and stability to the hollow spheres while maintaining surface functional groups for further modifications. The resulting hollow spheres showed a higher loading efficiency of plasmid DNA (pDNA) by using polyplex (~ 70 μg pDNA/mg of hollow sphere) than that of self-assembled ELP particles and demonstrated controlled release triggered by protease and elastase. Moreover, polyplex-loaded hollow spheres showed better cell viability than polyplex alone and yielded higher luciferase expression by providing protection against endosomal degradation. Overall, the monodispersed, tunable hollow spheres with a capability of post-functionalization can provide an exciting new opportunity for use in a range of therapeutic and diagnostic applications.
KeywordsElastin-like polypeptide; Hollow sphere and transfection; Monodispersed; Self-assembly; Tunable
ANZSRC Field of Research 2020400302. Biomaterials
400311. Tissue engineering
Public NotesFiles associated with this item cannot be displayed due to copyright restrictions.
Byline AffiliationsNational University of Ireland, Ireland
Queen's University, Canada
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