Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot
Article
Dash, Biraja C., Mahor, Sunil, Carroll, Oliver, Mathew, Asha, Wang, Wenxin, Woodhouse, Kimberly A. and Pandit, Abhay. 2011. "Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot." Journal of Controlled Release. 152 (3), pp. 382-392. https://doi.org/10.1016/j.jconrel.2011.03.006
Article Title | Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot |
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ERA Journal ID | 14794 |
Article Category | Article |
Authors | Dash, Biraja C., Mahor, Sunil, Carroll, Oliver, Mathew, Asha, Wang, Wenxin, Woodhouse, Kimberly A. and Pandit, Abhay |
Journal Title | Journal of Controlled Release |
Journal Citation | 152 (3), pp. 382-392 |
Number of Pages | 11 |
Year | 2011 |
Publisher | Elsevier |
Place of Publication | Netherlands |
ISSN | 0168-3659 |
1873-4995 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.jconrel.2011.03.006 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0168365911001428 |
Abstract | Self-assembly driven processes can be utilized to produce a variety of nanostructures useful for various in vitro and in vivo applications. Characteristics such as size, stability, biocompatibility, high therapeutic loading and controlled delivery of these nanostructures are particularly crucial in relation to in vivo applications. In this study, we report the fabrication of tunable monodispersed elastin-like polypeptide (ELP) hollow spheres of 100, 300, 500 and 1000 nm by exploiting the self-assembly property and net positive charge of ELP. The microbial transglutaminase (mTGase) cross-linking provided robustness and stability to the hollow spheres while maintaining surface functional groups for further modifications. The resulting hollow spheres showed a higher loading efficiency of plasmid DNA (pDNA) by using polyplex (~ 70 μg pDNA/mg of hollow sphere) than that of self-assembled ELP particles and demonstrated controlled release triggered by protease and elastase. Moreover, polyplex-loaded hollow spheres showed better cell viability than polyplex alone and yielded higher luciferase expression by providing protection against endosomal degradation. Overall, the monodispersed, tunable hollow spheres with a capability of post-functionalization can provide an exciting new opportunity for use in a range of therapeutic and diagnostic applications. |
Keywords | Elastin-like polypeptide; Hollow sphere and transfection; Monodispersed; Self-assembly; Tunable |
ANZSRC Field of Research 2020 | 400302. Biomaterials |
400311. Tissue engineering | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | National University of Ireland, Ireland |
Queen's University, Canada |
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