Production of B-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture

Article

McCabe, Bernadette K., Kuek, Clem, Gordon, Geoffrey L. R. and Phillips, Michael W.. 2003. "Production of B-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture." Journal of Industrial Microbiology and Biotechnology. 30 (4), pp. 205-209. https://doi.org/10.1007/s10295-003-0033-5
Article Title

Production of B-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture

ERA Journal ID3367
Article CategoryArticle
AuthorsMcCabe, Bernadette K. (Author), Kuek, Clem (Author), Gordon, Geoffrey L. R. (Author) and Phillips, Michael W. (Author)
Journal TitleJournal of Industrial Microbiology and Biotechnology
Journal Citation30 (4), pp. 205-209
Number of Pages5
Year2003
Place of PublicationHeidelberg, Germany
ISSN1367-5435
1476-5535
Digital Object Identifier (DOI)https://doi.org/10.1007/s10295-003-0033-5
Abstract

Two anaerobic fungi, one a monocentric strain (Piromyces sp. KSX1) and the other a polycentric strain (Orpinomyces sp. 478P1), were immoblised in calcium alginate beads and cultured in sequential batches where spent medium (containing o.25% cellobiose) was repeatedly drained and replaced. B-Glucisudase production with KSX1 was maintained for 45 days over six repeated batch cultures yielding a maximum level of 107 mIU/ml. For 478P1, B-glucosidase production was maintained for 30 days over four repeated batches yielding a maximum level of 34 mIU/ml. Although repeat-batch cultures of KSX1 produced more B-glucosidase than strain 478P1, the maximum specific B-glucosidase produced from these immobilised cultures was similar. the immobilised polycentric strain proved to be operationally superior to strain KSX1, as strain 478P1 did not produce any growth in the culture liquor.

Keywordsanaerobic fungi; immobilisation; b-Glucosidase; repeat-batch culture
ANZSRC Field of Research 2020310803. Plant cell and molecular biology
310705. Mycology
310106. Enzymes
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Byline AffiliationsUniversity of Western Sydney
Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia
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