Growth and characterisation of Chlorella vulgaris under increased lipid culture regimes

Presentation

Harris, Peter, McCabe, Bernadette K. and Pittaway, Pam. 2010. "Growth and characterisation of Chlorella vulgaris under increased lipid culture regimes." Goh, Steven C. and Wang, Hao (ed.) 2010 Southern Region Engineering Conference (SREC 2010). Toowoomba, Australia 11 - 12 Nov 2010 Toowoomba, Australia.
Paper/Presentation Title

Growth and characterisation of Chlorella vulgaris under increased lipid culture regimes

Presentation TypePresentation
AuthorsHarris, Peter (Author), McCabe, Bernadette K. (Author) and Pittaway, Pam (Author)
EditorsGoh, Steven C. and Wang, Hao
Journal or Proceedings TitleProceedings of the 2010 Southern Region Engineering Conference (SREC 2010)
Year2010
Place of PublicationToowoomba, Australia
Web Address (URL) of Paperhttp://www.usq.edu.au/engsummit
Web Address (URL) of Conference Proceedingshttp://www.usq.edu.au/engsummit/proceedings
Conference/Event2010 Southern Region Engineering Conference (SREC 2010)
Event Details
2010 Southern Region Engineering Conference (SREC 2010)
Event Date
11 to end of 12 Nov 2010
Event Location
Toowoomba, Australia
Abstract

Microalgae lipids and fatty acids have a range of applications and have attracted much global attention in recent years for their potential use as a source of biodiesel. The lipid and fatty acid content of microalgae vary according to species used and the nutrient, environmental and developmental conditions in which cells are cultured. Specifically, certain stresses such as nutrient limitation have been reported to increase lipid content. There is little information regarding the effect of growth conditions, particularly the effect of iron, on lipid productivity including its content and composition. Studies investigating the effects of growth conditions on lipid content and composition are essential given that the distinction in the main lipid classes and fatty acid composition are important for the subsequent conversion of microalgal oils to biofuels.

This presentation will document a 1 year Science Honours project which is currently investigating the lipid classes and fatty acid composition of the freshwater microalgae Chlorella vulgaris during growth on media designed to increase lipid content. Proposed studies will involve a two phase batch culturing of the microalgae which utilises media for optimal growth in the first phase with subsequent transfer of the algae to media designed to increase lipid content.The specific media conditions to be investigated will include factors such as nitrogen limitation and addition of iron at different concentrations. Growth and lipid content will be measured under the various culture conditions and the cultures will be characterised in terms of lipid classes and fatty acid composition during periods of optimal lipid accumulation. To quantitatively analyse these factors, techniques including gravimetric analysis, in situ Nile Red determination of neutral lipids, and GC-MS analysis of extracted and transesterified fatty acids will be utilised. The results of nutrient limitation and addition will be reported for each culture condition and the specific effects on growth phase, lipid accumulation and composition will be presented under each different culture regime.

Keywordsbiodiesel, microalgae, Chlorella vulgaris, lipid optimisation, lipid and fatty acid analysis, biodiesel fuel efficiency
ANZSRC Field of Research 2020401102. Environmentally sustainable engineering
Byline AffiliationsNational Centre for Engineering in Agriculture
Department of Biological and Physical Sciences
Institution of OriginUniversity of Southern Queensland
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Pittaway, P., Hancock, N., Brink, G., Symes, T. and Schmidt, E.. 2009. "Revisiting artificial monolayers as a strategy to reduce evaporative loss from large open water storages ." Kenway, S. J. and Begbie, D. K. (ed.) 1st Urban Water Security Research Alliance Science Forum (UWSRA 2009). Brisbane, Australia 17 - 18 Aug 2009 Brisbane, Australia.
Sweetening a bitter pill: educational strategies supporting the foundation sciences in first year nursing
McCabe, Bernadette K.. 2009. "Sweetening a bitter pill: educational strategies supporting the foundation sciences in first year nursing." Mortimer, Kelly (ed.) 12th Pacific Rim First Year in Higher Education Conference (FYHE 2009). Townsville, Australia 29 Jun - 01 Jul 2009 Brisbane, Australia.
Immobilization of monocentric and polycentric types of anaerobic chytrid fungi in Ca-alginate
McCabe, Bernadette K., Kuek, Clem, Gordon, Geoffrey L. R. and Phillips, Michael W.. 2001. "Immobilization of monocentric and polycentric types of anaerobic chytrid fungi in Ca-alginate ." Enzyme and Microbial Technology. 29 (2-3), pp. 144-149. https://doi.org/10.1016/S0141-0229(01)00367-2
Introducing wine science in the high school curriculum: a teacher's workshop
McCabe, Bernadette K.. 2007. "Introducing wine science in the high school curriculum: a teacher's workshop." Burton, Tracey (ed.) 9th Biennial Chemical Education Conference (CHEMED 2007): Where Chemical Agents Bond. Auckland, New Zealand 01 - 04 Jul 2007 Auckland, New Zealand.
Effects of a suspended shade cloth cover on water quality of an agricultural reservoir for irrigation
Maestre-Valero, J. F., Martinez-Alvarez, V., Gallego-Elvira, B. and Pittaway, P.. 2011. "Effects of a suspended shade cloth cover on water quality of an agricultural reservoir for irrigation." Agricultural Water Management. 100 (1), pp. 70-75. https://doi.org/10.1016/j.agwat.2011.08.020
Microbial and environmental implications for use of monolayers to reduce evaporative loss from water storages
Pittaway, Pam and van den Ancker, Tania. 2010. Microbial and environmental implications for use of monolayers to reduce evaporative loss from water storages. Toowoomba, Australia. University of Southern Queensland.
Identifying threshold concepts specific to science learning in nursing and the development of teaching approaches to facilitate their crossing
McCabe, Bernadette K.. 2010. "Identifying threshold concepts specific to science learning in nursing and the development of teaching approaches to facilitate their crossing." 3rd Biennial Threshold Concepts Symposium: Exploring Transformative Dimensions of Threshold Concepts. Sydney, Australia 01 - 02 Jul 2010 Sydney, Australia.
Photodegradation of Australian freshwater microlayers
Stuckey, Nick, Pittaway, Pam and Larsen, Kim. 2010. "Photodegradation of Australian freshwater microlayers." Ghiggino, Kenneth P. and Smith, Gerald (ed.) 6th Asian Photochemistry Conference 2010. Wellington, New Zealand 14 - 18 Nov 2010
School-scientist partnerships: what makes them tick? A case study from the CSIRO scientists in schools program
McCabe, Bernadette K., Weber, Hellen and Pezaro, Charlotte. 2011. "School-scientist partnerships: what makes them tick? A case study from the CSIRO scientists in schools program." 1st international Conference of STEM in Education. Brisbane, Australia 26 - 27 Nov 2010 Brisbane, Australia.
Tablet PCs as a catalyst to pedagogical innovation: a case study in microbiology
McCabe, Bernadette K.. 2010. "Tablet PCs as a catalyst to pedagogical innovation: a case study in microbiology." 5th International Inquiring Pedagogies Conference: Conceptualising Impact: Exploring the Effectiveness of Practice Through Pedagogic Research (iPED 2010). Coventry, United Kingdom 15 - 16 Sep 2010 Coventry, United Kingdom.
Photodegradation of Australian freshwater microlayers and the implications for potable water management
Stuckey, Nicholas, Pittaway, Pam and Larsen, Kim. 2010. "Photodegradation of Australian freshwater microlayers and the implications for potable water management." Begbie, D. K. and Wakem, S. L. (ed.) 2nd Urban Water Security Research Alliance Science Forum and Stakeholder Engagement. Brisbane, Australia 28 - 29 Sep 2010 Brisbane, Australia.
Microalgae as alternative fuel for compression ignition (CI) Engines
Allwayzy, S. H., Yusaf, T., McCabe, B., Pittaway, P. and Aravinthan, V.. 2010. "Microalgae as alternative fuel for compression ignition (CI) Engines." Goh, Steven C. and Wang, Hao (ed.) 2010 Southern Region Engineering Conference (SREC 2010). Toowoomba, Australia 11 - 12 Nov 2010 Toowoomba, Australia.
Water quality performance criteria for monolayer product specifications: selecting the right product for the job
Pittaway, Pam. 2010. "Water quality performance criteria for monolayer product specifications: selecting the right product for the job ." Australian Irrigation Conference and Exibition 2010: One Water Many Futures. Sydney, Australia 08 - 10 Jun 2010 Sydney, Australia.
The impact of water quality on monolayer performance in reducing evaporative loss from water storages
Pittaway, Pam and van den Ancker, Tania. 2010. "The impact of water quality on monolayer performance in reducing evaporative loss from water storages." Australian Irrigation Conference and Exibition 2010: One Water Many Futures. Sydney, Australia 08 - 10 Jun 2010 Sydney, Australia.
Determining operational requirements to optimise monolayer performance on a farm dam via a 'Universal Design Framework'
Brink, Gavin, Wandel, Andrew P., Pittaway, Pam, Hancock, Nigel and Pather, Selvan. 2010. "Determining operational requirements to optimise monolayer performance on a farm dam via a 'Universal Design Framework'." Montagu, Kelvin (ed.) Australian Irrigation Conference and Exibition 2010: One Water Many Futures. Sydney, Australia 08 - 10 Jun 2010 Sydney, Australia.
Will artificial monolayers adversely affect water quality? Insights from twelve months of monitoring with no monolayer
Pittaway, Pam. 2010. "Will artificial monolayers adversely affect water quality? Insights from twelve months of monitoring with no monolayer ." Begbie, D. K. and Wakem, S. L. (ed.) 2nd Urban Water Security Research Alliance Science Forum and Stakeholder Engagement. Brisbane, Australia 28 - 29 Sep 2010 Brisbane, Australia.
Properties of natural microlayers on Australian freshwater storages and their potential to interact with artificial monolayers
Pittaway, Pam and van den Ancker, Tania. 2010. "Properties of natural microlayers on Australian freshwater storages and their potential to interact with artificial monolayers." Marine and Freshwater Research. 61 (10), pp. 1083-1091. https://doi.org/10.1071/MF09159
A ‘universal design framework’ for installation planning and operational management of evaporation-suppressing films on agricultural reservoirs
Brink, Gavin, Wandel, Andrew P., Pittaway, Pam, Hancock, Nigel and Pather, Selvan. 2010. "A ‘universal design framework’ for installation planning and operational management of evaporation-suppressing films on agricultural reservoirs." Genet, Roger (ed.) International Conference on Agricultural Engineering: Towards Environmental Technologies (2010). Clermont-Ferrand, France 06 - 08 Sep 2010
The combined use of shade-cloth covers and monolayers to prevent evaporation in irrigation reservoirs
Gallego-Elvira, B., Martinez Alvarez, V., Pittaway, Pam, Symes, Troy and Hancock, Nigel. 2010. "The combined use of shade-cloth covers and monolayers to prevent evaporation in irrigation reservoirs." International Conference on Agricultural Engineering: Towards Environmental Technologies (2010). Clermont-Ferrand, France 06 - 08 Sep 2010
Using a tablet PC in bioscience instruction amongst diverse student cohorts
McCabe, Bernadette K.. 2009. "Using a tablet PC in bioscience instruction amongst diverse student cohorts." Franke, Katharina (ed.) 2009 Australasian Tablets in Education Conference. Clayton, Australia 03 - 04 Dec 2009 Melbourne, Australia.
Ready, set, go...- flexible package supporting the biophysical sciences in first year nursing
McCabe, Bernadette K.. 2009. "Ready, set, go...- flexible package supporting the biophysical sciences in first year nursing." Kift, Sally (ed.) ALTC First Year Experience Curriculum Design Symposium 2009. Brisbane, Australia 05 - 06 Feb 2009 Brisbane, Australia.
Towards a biophysical understanding of observed performance of evaporation suppressant films applied to agricultural water storages - first analyses
Hancock, Nigel, Pittaway, Pam and Symes, Troy. 2009. "Towards a biophysical understanding of observed performance of evaporation suppressant films applied to agricultural water storages - first analyses ." Banhazi, T. M. and Saunders, C. (ed.) SEAg 2009: Agricultural Technologies In a Changing Climate. Brisbane, Australia 13 - 16 Sep 2009 Brisbane, Australia.
Smart monolayer application and management to reduce evaporation on farm dams - formulation of a universal design framework
Brink, Gavin, Symes, Troy, Pittaway, Pam, Hancock, Nigel, Pather, Selvan and Schmidt, Erik. 2009. "Smart monolayer application and management to reduce evaporation on farm dams - formulation of a universal design framework." Venkatraman, K. (ed.) 2009 Environmental Research Event. Noosa, Australia 10 - 13 May 2009
Improving the performance of chemical monolayers in reducing farm dam evaporation
Craig, Ian, Schmidt, Erik, Hancock, Nigel, Barnes, Geoff, Turnbull, David J., Pittaway, Pam and Morrison, Pippa. 2006. "Improving the performance of chemical monolayers in reducing farm dam evaporation." 3rd CRC-IF Annual Research Forum. Narrabri, Australia 05 - 06 Dec 2006 Toowoomba, Queensland.
Under the microscope: from bugs to the class room
McCabe, Bernadette K.. 2007. "Under the microscope: from bugs to the class room." Fogarty, Roderick (ed.) 3rd International Conference on Pedagogies and Learning. Brisbane, Australia 26 - 28 Sep 2007 Toowoomba, Australia.
Evaporation, seepage and water quality management in storage dams: a review of research methods
Craig, Ian, Aravinthan, Vasantha, Baillie, Craig Peter, Beswick, Alan, Barnes, Geoff, Bradbury, Ron, Connell, Luke, Cooper, Paul, Fellows, Christopher, Fitzmaurice, Li, Foley, Joseph P., Hancock, Nigel, Lamb, David, Morrison, Pippa, Mossad, Ruth, Misra, R. K., Pittaway, Pam, Prime, Emma, Rees, Steve, ..., Turnbull, David. 2007. "Evaporation, seepage and water quality management in storage dams: a review of research methods." Environmental Health. 7 (3), pp. 84-97.
Parallels of lived experiences in learning: a scientist learning qualitative research and nurses learning science
McCabe, Bernadette K.. 2007. "Parallels of lived experiences in learning: a scientist learning qualitative research and nurses learning science." International Journal of Pedagogies and Learning. 3 (1), pp. 13-20.
Production of B-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture
McCabe, Bernadette K., Kuek, Clem, Gordon, Geoffrey L. R. and Phillips, Michael W.. 2003. "Production of B-glucosidase using immobilised Piromyces sp. KSX1 and Orpinomyces sp. 478P1 in repeat-batch culture." Journal of Industrial Microbiology and Biotechnology. 30 (4), pp. 205-209. https://doi.org/10.1007/s10295-003-0033-5